Summary
Overview
Work History
Education
Skills
Certification
Scientific Publications
Guest Lecturer Presentaciones
Timeline
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RODOLFO EDUARDO GAMEZ SAZO (EDWARD)

RODOLFO EDUARDO GAMEZ SAZO (EDWARD)

Ph.D. MD
Rochester,NY

Summary

Skill in integrative biomedical science, with expertise in tissue-engineering regenerative medicine, local drug release (chemotherapy, neurotrophic factors, extracellular matrixes, growth factors), and fabrication of biodegradable scaffolds applying photopolymerization technology and cell cultures for nerve tissue regeneration in the central and peripheral nervous system research studies basic science. Skills in microsurgery techniques scaffold transplantation devices (nerve guidance tube, microchannel cylindrical guidance) with or without progenitors glia cell enhancing myelination and axonal regeneration in peripheral and spinal cord nerve injuries.

Knowledgeable researcher using universal automated testing hydraulic systems to perform static testing, including tensile, compression, bend, peel, tear, shear, friction, puncture, and other mechanical tests, skills in lyophilization technology protocols synthesizing styrene-derivatized gelatin from collagen to create lyophilized proteins and biologics products (freeze-drying) by applying freeze-dryer benchtop configurator equipment (Labconco industrial equipment). Laboratory experience in organic chemistry synthesizing and extracting solvents, water evaporation, dialysis, and melting point from chemical compounds and drug formulation (e.g., water-soluble carboxylated champorquinone photocrosslinking agent) using reflux rotary evaporation system (BUCHI's Rotavapor® R-100).

A well-organized scientist researcher and healthcare professional outcomes and report data collection for manuscripts publication and grant submission to research grant organizations and project submission approvals. Talented professional scientist dedicated to biomedical engineering, tissue engineering, local drug delivery technology, and neuroscience research, offering hands-on experience supporting research efforts and completing independent or as part of team leader projects with first-author high-quality scientific papers for publication in peer-reviewed journals (Cell Transplantation, Biomaterials Journals, and Journal Neuroscience Research). Trilingual language speaking and writing communication skills proficiency (English, Spanish and Japanese), understanding equity, inclusion, and diversity in university settings environment, supporting student ambition and goals in science development careers, understanding their goals and dreams from different nationalities, cultures, and citizen origins preparing them for future endeavors contributing to developing high skills and knowledge in scientific research development technology. Excellent organization and planning abilities with a firm commitment to accuracy and integrity of work.

Well-versed in using Microsoft Word, PowerPoint, Excell, Keynote, and Photoshop to present outcomes data for a poster or oral presentations at national or international meetings at different scientific research societies (Japan, USA, Latin America). A scientist researcher with a firm commitment to accuracy and integrity of work, looking for a dynamic position in tissue engineering and regenerative medicine at a University teaching academic, Pharmaceutical, or laboratory manufacturing technology organization.

Overview

1
1
Certification

Work History

Postdoctoral Research Associate

Neurosurgery Department University of Rochester
Rochester, NY
03.2011 - 08.2011
  • 2005-2010-Postdoctoral Associate Research. The Miami Project to Cure Paralysis” and The Buoniconti Fund, Lois Pope Life Center, University of Miami Leonard M. Miller School of Medicine, Neurosurgery Department, Miami FL.
  • 2003-2004-Postdoctoral Research Fellowship. Japanese Society for the Promotion of Sciences (JSPS) Biomedical Engineering and Neurosurgery Departments, Graduate School of Medical Sciences Kyushu University. Fukuoka Japan.
  • 1998- Guatemalan Institute of Social Security Certifies: DR. RODOLFO E GAMEZ worked as Specialized Physician I in Neurosurgery and Spinal Column at the Escuintla Hospital, Guatemala City. C.A.
  • 1994-School of Psychological Sciences, University of San Carlos of Guatemala, hereby Certifies Rodolfo Eduardo Gamez Sazo., MD. Worked for this Academic Unit, as Deputy Professor, acting as Temporary Professor Guatemala C.A.
  • 1987- The Executive Board School of Faculty of Psychological Sciences University of San Carlos of Guatemala Nominate the Student Rodolfo Eduardo Gamez Sazo from the Faculty of Medicine to occupy the position of Assistant Professor Ad-Honorem Services in the Basic Training Level Subject of Biology I for the Academic year 1987. Guatemala C.A.
  • 1996- The Military Medical Center, Guatemala ARMY Certify: Dr. Rodolfo E Gamez Sazo worked in the Surgery Department as General Surgeon assisting in General Surgery and Neurosurgery surgical procedures efficiently and responsibly. Military Medical Center, General Hospital Direction Department. Guatemala, C. A.
  • 1989-The Board of Directors of the “Brother Pedro Sanatorium” and the Sub-Director (Dr. Enio Betancourt) Certify: Eduardo Gamez, MD, occupied the position of Medical Doctor. Sor Thelma Moran R. Executive Director. Guatemala, C.A.
  • 1989-The Bella Aurora Hospital and the Scientific Commission certify: that Dr. Eduardo Gamez Sazo was elected to cover internally (Interim Physician) the vacation of Dr. Rafael Nunez Garcia. Sign Chief Resident Dr. Jose C. Avila Palma. Guatemala, C.A.

Education

Ph.D. - Integrative Biomedical Sciences (Medical Science)

Kyushu University School of Medicine
3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582 Japan
03.2004

M.D. - General Surgery Residency

University of San Carlos of Guatemala, C.A.
Guatemala City
01.1994

M.D. - Medical Science

University of San Carlos, Faculty of Medicine
Guatemala C.A.
10.1988

Skills

  • Language spoken: English, Spanish and Japanese
  • Surgical: Microsurgery, Transplantation devices for research projects in Peripheral and Spinal Cord Nerve injuries
  • Tissue-Engineering Fabrication Biodegradable Devices
  • Specialized Techniques: Confocal microscopy Fluorescence microscopy Transmission electron microscopy Time-lapse recordings
  • Human and animal tissue culture: Harvest animal embryonic and adult ganglions organ, Schwann cells, and ganglion peripheral nerve
  • Tissue-Engineering Techniques
BiomaterialsphotocrosslinkingchamporquinoneLabconcoconfiguratorbiologic'sderivatizedlyophilization
  • Photopolymerization of biodegradable scaffolds polymers fabrication for tissue engineering regeneration in vitro/in vivo settings Synthesis of gelatin from collage type I In Situ photo-polymerization drug release technology Micro-Transplantation of engineered cells into scaffolds Tissue engineering delivery of neurotrophic facrosslinkingtracellular matrix proLyophilizationcellular matrix proteins immobilization by photo-croderivatizeof collagen type I Lyophilization technique of type I collagen Dialysis of styrene derivatize gelatin type I collagen Measurement of Mechanical properties of the polymer (elasticity, stress-strain, compression, and breaking point) Synthesis of photo-crosslink Camphorquinone using Büchi® Rotavapor® R-210 evaporator with jack and water bath, 29/32 joint, 240V
  • Experimental Protocols
  • Axonal outgrowth and myelination cell cultures studies in vitro/in vivo Human and animal sural nerve harvesting for Schwann cell isolation culture in vitro/in vivo applications Cryopreservation protocol, freezing cells Quantitation (hemocytometer, electronic cell counting)
  • DNA estimation by Hoechst immunostaining
  • Immunostaining of cells estimation by using primary and secondary antibodies Alexa Flour Dyes protocols
  • Toluidine blue, Giemsa stain, phase contrast microscopy

Certification

  • Ph.D. Philosophical Degree (Medical Science) - 2004
  • M.D. Medical Doctor (Physician and Surgeon-College of Physicians and Surgeons of Guatemala City, License #7084) - 1988
  • Expert Accounting (Ministry of Education, Guatemala City, License#24379 - 1977

Scientific Publications

  • Cell Transplantation, Vol. 12, pp. 481–490, 2003. Photoconstructs of Nerve Guidance Prosthesis Using Photoreactive Gelatin as a Scaffold. E. Gamez, MD., K. Ikezaki, MD., PH.D., M. Fukui, MD., PH.D., and T. Matsuda, PH.D. Department of Neurosurgery and Department of Biomedical Engineering, Graduate School of Medicine, Kyushu University, 3-1-1, Maidashi, Higashi-Ku, Fukuoka 812-8582, Japan.
  • Cell Transplantation, Vol. 13, pp. 549–564, 2004. Photofabricated Gelatin-Based Nerve Conduits: Nerve Tissue Regeneration Potentials Eduardo Gamez, MD., PH.D., Yoshinobu Goto, MD., PH.D., Kengo Nagata., D.D.S., PH.D., Toru Iwaki., MD., PH.D., Tomio Sasaki., MD., PH.D., and Takehisa Matsuda., PH.D. Graduate School of Medicine, Kyushu University, 3-1-1, Maidashi, Higashi-Ku, Fukuoka 812-8582, Japan.
  • J Neurosci Res. 2010 March ; 88(4): 712–721. Rapid Assessment of Internodal Myelin Integrity in CNS Tissue. Daniel A. Kirschner, Ph.D. (1), Robin L. Avila, Ph.D. (1), Rodolfo E. Gamez Sazo, M.D., Ph.D. (2), Adrienne Luoma, B.A. (1), Gaby U. Enzmann, Ph.D. (3), Deepika Agrawal (1), Hideyo Inouye, Ph.D. (1), Mary Bartlett Bunge, Ph.D. (2), Jeffery Kocsis, Ph.D. (4), Alan Peters, Ph.D. (5), and Scott R. Whittemore, Ph.D. (3). 1 Biology Department, Boston College, Chestnut Hill, MA. 2 The Miami Project to Cure Paralysis, NW 14th Terrace, Miami, FL. 3 Department of Neurological Surgery, University of Louisville School of Medicine, Louisville, KY. 4 Department of Neurology and Center for Neuroscience and Regeneration Research, Yale University School of Medicine, New Haven, CT. 5 Department of Anatomy and Neurobiology, Boston University School of Medicine, Boston, MA.
  • Biomaterials. 2012 November; 33(33): 8529–8539. Fabrication of Growth Factor-and Extracellular Matrix-Loaded, Gelatin-Based Scaffolds, and their Biocompatibility with Schwann Cells and Dorsal Root Ganglia . Rodolfo E. Gámez Sazo (a), Katsumi Maenaka (b), Weiyong Gu (c), Patrick M. Wood (a), (d), and Mary Bartlett Bunge (a,d,e,)., a The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL 33136-0621, USA., b Laboratory of Biomolecular Science, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, 060-0812, Japan., c Tissue Biomechanics Laboratory, Department of Biomedical Engineering, College of Engineering, University of Miami, Coral Gables, FL 33124-0621, USA., d Department of Neurological Surgery, University of Miami Miller School of Medicine, Miami, FL 33136-0621, USA., e Department of Cell Biology, University of Miami Miller School of Medicine, Miami, FL 33136-0621, USA.

Guest Lecturer Presentaciones

  • 2011- Oral Presentation “Photocurable Scaffolds Derivatized Tissue-Engineering and Drug Delivery for Peripheral and Spinal Cord Injury in vitro/in vivo Outcomes” Department of Neurosurgery & Center for Neural Development and Disease University of Rochester, Medical Center, NY.
  • 2010- Oral Presentation Department of Biomedical Engineering “Styrene Tissue-Engineering Derivatized Scaffold Fabrication Drug released of Neurotrophic Factors for Peripheral and Spinal Cord Tissue Regeneration in vitro/in vivo Outcomes” Hajim School of Engineering & Applied Sciences. University of Rochester, NY.
  • 2010- Oral Presentation Biodegradable Tissue-Engineering Conduits Guidance Fabrication for Peripheral and Central Nervous System Tissue Regeneration. Center for Neural Repair and Rehabilitation, Shriner's Hospitals Pediatric Research Center, Temple University School of Medicine, Philadelphia, PA.
  • October 3th, 2009-Laboratory of Biomolecular Science, Faculty of Pharmaceutical Sciences, Hokkaido University, Japan, Certify: Rodolfo E Gamez Sazo MD, Ph.D. Presented at the Seminar Lectures: New Nerve Guidance Conduits Tissue-Engineering Drug Released Technology of Neurotrophic Factors and ECM for Spinal Cord Injuries Regeneration. Laboratory of Biomolecular Science, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-Ku, Sapporo 060-0812, Japan.
  • September 6th, 2006-The Miami Project to Cure Paralysis Lois Pope LIFE Center, Buoniconti Fund, and The Neuroscience Center Seminars Certify Rodolfo E Gamez Sazo MD, Ph.D. Presented at the Morning Seminar Lectures: New Nerve Guidance Conduits for Peripheral Nerve Regeneration: Photofabrication and In vivo Performance, University of Miami Leonard Miller School of Medicine, Department of Neurosurgery, Miami, Florida.

Timeline

Postdoctoral Research Associate

Neurosurgery Department University of Rochester
03.2011 - 08.2011

Ph.D. - Integrative Biomedical Sciences (Medical Science)

Kyushu University School of Medicine

M.D. - General Surgery Residency

University of San Carlos of Guatemala, C.A.

M.D. - Medical Science

University of San Carlos, Faculty of Medicine
RODOLFO EDUARDO GAMEZ SAZO (EDWARD)Ph.D. MD