Elena Scut
San Francisco,CA
Summary
A passionate and highly motivated scientist that thrives in a competitive and fast-paced environment. Accountability and attention to detail are among the core strengths.
Overview
6
6
years of professional experience
Work History
Scientist I - Cell Line Development (promotion)
National Resilience Inc.
04.2021 - 12.2023
- Generated stable over 20 CHO pools from construct preparation through fed-batch screening.
- Isolated over 50 high-expressing monoclonal cell lines by limiting dilution cloning and single cell printing followed by clone screening.
- Operated 2 small scale Eppendorf single use bioreactors in parallel, a total of 10 runs.
- Determined protein titer using Octet from harvest samples.
- Generated over 60 research cell banks.
- Performed RT-PCR sequence verification of transgenes.
- Summarized and presented data internally and to clients.
- Maintained lab equipment and managed inventory.
- Trained other scientists and supervised research associates.
Associate Scientist - Process Development (Cell Therapy)
Ology Bioservices Inc. (now National Resilience)
10.2020 - 04.2021
- Processed whole blood and leukapheresis materials.
- Isolated and expanded T and B cells.
- Assessed T and B cell purity after enrichment with flow cytometry.
- Transduced activated T cells with Lentivirus vectors.
- Harvested and banked T and B cells after transduction and expansion.
- Initiated, cultured, and banked human leukemia cell lines (K562, K562-GFP, KG-1a).
- Analyzed and presented flow cytometry data internally and to clients.
Research Technician - Process Development
Ottawa Hospital Research Institute
09.2019 - 06.2020
- Optimized the upstream lentivirus (LV) production process.
- Assembled and operated iCELLis Nano Bioreactors throughout LV production runs in adherent HEK293T cells.
- Trained and supervised employees on LV production.
- Involved in Vaccinia Virus production process on client demand.
- Experienced with the preparation of cell culture media for large scale experiments.
- Followed Good Laboratory Practices (GLP) and Good Documentation Practices (GDP).
Graduate MSc Student
Dr. Rebecca Auer's Laboratory, Center for Innovative Cancer Research
09.2017 - 09.2019
- Developed leukemia cell vaccines by altering their immunogenicity using oncolytic Rhabdoviruses.
- Characterized the innate and adaptive immune responses following vaccination using multi-color flow cytometry, cell sorting, ELISAs, and Multiplex cytokine/chemokine assays using Luminex xMAP technology.
- Processed blood samples collected from both healthy individuals and patients receiving leukemia treatment after bone marrow transplantation as part of pre-clinical study to evaluate vaccination regimens for immunocompromised cancer patients.
- Presented my research at the International Summit for Cancer Immunotherapy in 2018 (Banff, Alberta, Canada) and 2019 (Victoria, British Columbia, Canada).
Education
Master of Science - Biochemistry, Human and Molecular Genetics
University of Ottawa
Ottawa, Canada06.2020
Bachelor of Science - Biochemistry, Immunology and Microbiology
University of Ottawa
12.2017
Skills
Mammalian Tissue Culture
Processing Primary tissue
Transfection/Transduction
Small Scale Bioreactors
Antibody production and quantification (Octet)
Cell Banking
DNA/RNA extraction and purification
PCR/qPCR/RT-PCR
Sanger Sequencing (SnapGene)
ELISA
Languages
Romanian
Native or Bilingual
Russian
Full Professional
English
Native or Bilingual
Timeline
Scientist I - Cell Line Development (promotion)
National Resilience Inc.
04.2021 - 12.2023
Associate Scientist - Process Development (Cell Therapy)
Ology Bioservices Inc. (now National Resilience)
10.2020 - 04.2021
Research Technician - Process Development
Ottawa Hospital Research Institute
09.2019 - 06.2020
Graduate MSc Student
Dr. Rebecca Auer's Laboratory, Center for Innovative Cancer Research
09.2017 - 09.2019
Master of Science - Biochemistry, Human and Molecular Genetics
University of Ottawa
Bachelor of Science - Biochemistry, Immunology and Microbiology
University of Ottawa
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