Research Interests; HIV cure research, Immunology of infectious diseases and cancer therapeutics. Understanding drivers of deferential immune responses to similar immunogens and hence correlates of protection. I am a research scientist with over 15 years of progressive experience conducting research on the bench and leading teams. Successful at implementing research strategies and improving team performance . Strong background in qualitative and quantitative research. Ready to apply talents to rewarding new opportunity in similar scientific roles.
Overview
20
20
years of professional experience
Work History
Research Fellow
Emory University School of Medicine
Atlanta, USA
01.2022 - Current
Perform assay techniques as required under minimal supervision and operate specific equipment like the BD Symphony Multi color flow cytometer
Cytokine measurement with multiplex ELISA using the Mesoscale Discovery Platform (MSD)
Peripheral Blood Mononuclear Cells (PBMC) Isolation from whole blood
Culturing and cryopreservation of ex vivo immune cells and/or primary cell lines
Designing and evaluating of experiments for the RID HIV project
Develop new ideas that promote current research
Assist in Preparing of scientific manuscripts under the direction of the Principal Investigator
Train new recruits on current techniques
Basic Bioinformatics data analysis and generation of data graphs with Prism
Shipment coordinator
(charging dry shippers and shipment monitoring)
Freezer /sample manager (Manage freezer space and the in and out flow of study samples)
Prepare samples for next generation sequencing (RNA/DNA extraction, cDNA synthesis and Library preparation)
Lead person in monitoring the refilling of Carts with consumables in the Tissue culture rooms
Making general stock orders through Emory Express
Training new members in the proper use/operation of laboratory techniques and equipment
Reviewing literature for related research developments and techniques and advise current approach by the laboratory
Assist in generating of data figures and writing of publications with the principal investigator and team
Ensure safe handling and disposal of biohazard material and chemicals.
Laboratory Manager
Uganda Virus Research Institute – International AIDS Vaccine Initiative (UVRI-IAVI) HIV Vaccine program
Entebbe, Uganda
01.2019 - 12.2021
Oversee day to day operations of the lab and ensure that problems encountered are resolved timely
Reviewing research protocols to identify and plan for implications on lab operations, writing of protocol specific lab analytical plans
Ensure laboratory compliance with Good clinical Lab practice and that accreditation is maintained
Ensure laboratory compliance with Uganda virus institutional policies and safety regulations
Supervises staff (generally ten or more employees) in a large and/or complex research laboratory
Responsible for administrative, financial, staffing and planning aspects of the lab department
Supervising execution and completion of activities in the flow cytometry section as per schedule of work drawn by the Laboratory Manager
Ensuring that all test results (raw and result reports) are brought to the Laboratory Manager or Senior Scientist for Review and Approval
Monitor Testing turnaround times in the Flow Cytometry section to ensure test result reporting is not delayed
Manage stocks for reagents and consumables (both quantities and expiration dates) used in the Flow Cytometry Section
Ensure that reagents are batch tested and recorded appropriately
Spearhead installation/validation of new equipment/assays in the Flow Cytometry Section of the laboratory
Oversee the technical training of interns, new and old laboratory staff or visitors prior to their starting specimen testing/ assay running in the Flow Cytometry section
Give technical support to scientist/personnel requiring such assistance while using the LSR II
Help in drawing costs associated with use of the Flow Cytometry section by other organizations/scientists
Supervise equipment maintenance; ensure maintenance logs are completed in the Flow Cytometry room
Ensure that routine External Quality Assessment specimens in the Flow Cytometry Section are run and reported in time
Contribute to maintaining of the general laboratory supplies and reagent stocks.
Program Safety Manager/ Laboratory Quality manager
UVRI IAVI HIV Vaccine Program
Entebbe, Uganda
01.2012 - 08.2015
Oversee the safety of the UVRI-IAVI laboratory personnel and ensuring Lab quality systems are improved and accreditation is maintained.
Supervising technical staff during bench testing of clinical and research specimens
Conduct technical trainings of Laboratory personnel and interns
Ensuring reagents and kits and sample inventory records are correctly prepared/updated, kept at correct temperature
Ensure that that laboratory equipment is routinely maintained, cleaned, and operating as per manufacturers recommendations.
Laboratory Technologist
UVRI IAVI HIV Vaccine program
Entebbe, Uganda
12.2005 - 12.2007
Perform sample test assays following SOP
Laboratory Technologist
LMK Medical Lab and Consultancies
Kampala, Uganda
08.2004 - 11.2005
Perform sample test assays following SOP
Education
Master of Science - M.Sc. Molecular Biology and Biotechnology
Makerere University
Kampala, Uganda
02-2020
Bachelor of Science - Medical Laboratory Sciences
Mbarara University of Science And Technology
Mbarara, Uganda
04-2004
Skills
Multi color Flow Cytometry
Cytokine multiplex ELISA
Ex vivo immune cells and/or primary cell line cultures
Antibody neutralization (Plague forming units)
RNA/DNA extraction
CDNA synthesis and library preparation
Laboratory Safety
QA and QC
Good Clinical Lab Practice
BSL2 and BSL3 working
Immunology
Molecular Biology Lab management/Training and Supervision
Manuscript writing
Clinical trials
Computer skills
Data analysis: Flowjo, R, Prism Excel
Cryogenic Shipment
Analytical plans and SOPs writting
Affiliations
I like Playing the guitar and outdoor games
Publications
Immune activation alters cellular and humoral responses to yellow fever 17D vaccine, Enoch Muyanja, Aloysius Ssemaganda, Pearline Ngauv, Rafael Cubas, Helene Perrin, Divya Srinivasan, Glenda Canderan, Benton Lawson, Jakub Kopycinski, Amanda S. Graham, Dawne K. Rowe, Michaela J. Smith, Sharon Isern, Scott Michael, Guido Silvestri, Thomas H. Vanderford, Erika Castro, Giuseppe Pantaleo, Joel Singer, Jill Gillmour, Noah Kiwanuka, Annet Nanvubya, Claudia Schmidt, Josephine Birungi, Josephine Cox, Elias K. Haddad, Pontiano Kaleebu, Patricia Fast, Rafick-Pierre Sekaly, Lydie Trautmann, Journal of Clin Invest. 2014; 124(7)
An assessment of fishing communities around Lake Victoria, Uganda, as potential populations for future HIV vaccine efficacy studies: an observational cohort study, Noah Kiwanuka, Juliet Mpendo, Annet Nalutaaya, Matthias Wambuzi, Annet Nanvubya, Paul K Kitandwe, Enoch Muyanja, Julius Ssempiira, Apolo Balyegisawa, Ali Ssetaala, BMC Public Health. (Impact Factor: 2.08). 09/2014
Schistosoma mansoni infection in Ugandan men is associated with increased abundance and function of HIV target cells in blood, but not the foreskin: a cross-sectional study, Jessica L. Prodger, Aloysious Ssemaganda, Ali Ssetaala, Paul K. Kitandwe, Enoch Muyanja, Juliet Mpendo, Annet Nanvubya, Mathias Wambuzi, Leslie Nielsen, Noah Kiwanuka, Rupert Kaul, PLOS Neglected Tropical Diseases (Aug 2015)
Performance of an IAVI-African Network of Clinical Research Laboratories in Standardized ELISpot and Peripheral Blood Mononuclear Cell Processing in Support of HIV Vaccine Clinical Trials, Robert Langat, Bashir Farah, Jackton Indangasi, Simon Ogola, Gloria Omosa-Manyonyi, Omu Anzala, Jean Bizimana, Emmanuel Tekirya, Caroline Ngetsa, Moses Silwamba, Enoch Muyanja, Paramesh Chetty, Maureen Jangano, Nancy Hills, Jill Gilmour, Len Dally, Josephine Cox, Peter Hayes, (13 APR 2018) Bio Rxivpre-print: doi: https://doi.org/10.1101/
Characterization of CD4+ T cell subsets and HIV susceptibility in the inner and outer foreskin of Ugandan men, Galiwango RM, Yegorov S, Joag V, Prodger J, Shahabi K, Huibner S, Muyanja E, Kabuubi BR, Namuniina A, Nalutaaya A, Ssemaganda A, Lutwama F, Kitandwe PK, Nanvubya A, Mpendo J, Bagaya B, Kiwanuka N, Kaul R, Am J Reprod Immunol. EpubMay 24. (2019)
Hepatitis B prevalence and incidence in the fishing communities of Lake Victoria, Uganda: a retrospective cohort study, Paul Kato Kitandwe, Enoch Muyanja, Teddy Nakaweesa, Annet Nanvubya, Ali Ssetaala, Juliet Mpendo, Brenda Okech, Bernard S. Bagaya, Noah Kiwanuka, Matt A. Price, BMC Public Health. BMC Public Health https://doi.org/10.1186/s12889-021-10428-1 21:394 02(2021)
Schistosoma mansoni infection alters the host pre-vaccination environment resulting in blunted Hepatitis B vaccination immune responses, Roshell Muir, Talibah Metcalf, Slim Fourati, Yannic Bartsch, Jacqueline KyosiimireLugemwa, Glenda Canderan, Galit Alter, Enoch Muyanja, Brenda Okech, Teddy Namatovu, Irene Namara, Annemarie Namuniina, Ali Ssetaala, Juliet Mpendo, Annet Nanvubya, Paul Kato Kitandwe, Bernard S. Bagaya, Noah Kiwanuka, Jacent Nassuna, Victoria Menya Biribawa, Alison M. Elliott, Claudia J. de Dood, William Senyonga, Priscilla Balungi, Pontiano Kaleebu, Yunia Mayanja, Matthew Odongo, Jennifer Connor, Pat Fast, Matt A. Price, Paul L. A. M. Corstjens, Govert J. van Dam, Anatoli Kamali, Rafick Pierre Sekaly, Elias K. Hadda, PLoS Negl Trop Dis.17(7): e0011089. https://doi.org/ 10.1371/journal.pntd.0011089 (July 5, 2023)
Proportion of Ugandans with pre-pandemic SARS-CoV-2 cross-reactive CD4+ and CD8+ Tcell responses: A pilot study, Annemarie Namuniina, Enoch S. Muyanja, Victoria M. Biribawa, Brenda A. Okech, Aloysious Ssemaganda, Matt A. Price, Nancy Hills, Ann Nanteza, Bernard Ssentalo Bagaya, Daniela Weiskopf, Catherine Riou, Steven J. Reynolds, Ronald M. Galiwango, Andrew D. Redd, PLOS Glob Public Health3(8): e0001566. https://doi.org/10.1371/journal. pgph.0001566 (August 16, 2023)
Alcohol Consumption Modifies Susceptibility to HIV-1 Entry in Cervical Mucosa-Derived CD4+ T cells of Women Resident in a Fishing Community of Lake Victoria, Uganda, Bernard Ssentalo Bagaya, Emmanuella Driciru, Muwanda Fahad, Mary Nantongo, Marion Namuleme, Paul Kato Kitandwe, Enoch Muyanja Ssekayita, Ronald Galiwango, Violet Mirembe, Barbarah Kawoozo Muwenda, Moses Muwanga, Alex Kayongo, Fredrick Lutwama, PREPRINT
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