Erzsebet Szilagyi MD.PhD
Summary
Highly skilled physician scientist with 15 years of academic experience achieving patent, grant support and publications as well as long record of graduate and postgraduate student mentoring.
Overview
12
12
years of professional experience
Work History
Research Assistant Professor
University Of Illinois At Chicago
2015.01 - 2017.07
My research objective was to find an efficient stem cell therapy for Duchene Muscular Dystrophy through the creation of chimeric cells, created via ex-vivo PEG fusion of healthy and DMD affected myoblasts and mesenchymal stem cell. The two-year research project started from in vitro chimeric cell creation and analysis through preclinical administration-route testing in mdx/scid mice and resulted in three publication and ongoing grant support.
My work consisted in:
- Leading research activities and preparation of manuscripts related to Duchene Muscular Dystrophy and stem cell therapies
- Supervised medical student and orthopedic resident research activities
- Developed instructional plans and performed microsurgical training sessions for orthopedic residents in training (5 day session offered 2-3 times /academic year).
- Grant writing and submission (DMD, NIH, DOD, AHA, JDRF and other funding sources)
- IRB submission of clinical trial and ACC protocol submissions of basic research activities
Visiting Research Assistant Professor
University of Illinois at Chicago
2011.01 - 2015.04
My goal was to complete pre-clinical testing of Mesenchymal Stem Cells as immunomodulators in an allogeneic organ transplant.· The non-human primate kidney transplant study highlighted that MSC immunomodulation is strongly influenced by recipient immune activation state. My research interest were also focused on the effect of MSC in enhancing wound healing and the possible mediators and molecular pathways involved, resulting in publications and numerous poster presentations.
Moreover, my work role included:
- Study director of NIH non-human primate kidney transplant study (expert in management of all aspects, from animal maintenance to surgeries, data collection and report presentation)
- Laboratory resources management, project coordination, policy compliance
- Laboratory personnel management and medical student mentoring
- Abdominal and vascular surgery in non‐human primates, minipigs and rodents
- Study director of BARDA (DOD) (GLP) study testing effects of PTH on irradiation injury in rodents
Postdoctoral Research Fellow
University of Illinois at Chicago
2007.06 - 2011.01
My research project , under the supervision of PI: Prof . Amelia Bartholomew, focused on testing efficacy of MSCs as immunomodulators in prolonging allogeneic organ transplant survival in mice. Results of this project granted the support for an R01 multicenter non-human preclinical study.
Moreover, my work role included:
- Pathology specialist on GLP study of effect of irradiation on non-human primates
- Designed and performed MSC research projects in vitro and in vivo
- Abdominal and vascular surgery in non‐human primates, minipigs
- Microsurgery in rodents (heart transplant, ischemia-‐reperfusion, wound healing models)
- Flow cytometry, Multiplex, ELISA, cellular imaging (IMF, confocal, live, time‐lapse)
- GMP level cell cultures
Postdoctoral Research Fellow (joint)
University of Illinois at Chicago
2009.01 - 2010.12
- PI: Prof. Jose Oberholzer
- Clinical pancreatic islet isolation – GMP compliance
- GMP clinical donor pancreas preparation, density gradient centrifugation, islet cultures and GMP quality control
Postdoctoral Research Fellow
University of Pisa
2006.01 - 2007.01
Pursued independent and complementary research interests in detecting transplanted pancreatic islets and stem cells by MRI under the supervision of PI: Prof. Biancamaria Longoni, Prof. Franco Mosca, resulting in manuscript and numerous poster presentations.
- Designed and performed in vitro and in vivo research with the goal of developing pancreatic islet isolation, co-culture methods and transplant techniques.
- Drafted manuscripts and presented findings at European and World Transplantation conferences.
- Collaborated with four multidisciplinary team members to accomplish research goals.
Education
Ph.D. - Transplant Immunology, Surgery And Biotechnology
University of Pisa
Italy12.2005
M.D. -
Albert Szent-Gyorgyi Medical School
Szeged, Hungary09.2000
Skills
- Stem Cell Research
- Study Director
- Grant Writing
- Multidisciplinary Team Collaboration
- Developing GMP and GLP Protocols
- IRB and ACC compliance
- Mentoring
- Microsurgery
- Molecular Biology
- Cell cultures, Flow Cytometry and Immunohistochemisty
Educational Activities
- Instructor, Basic and Advanced Microsurgery Training Course for Orthopedic Surgery Residents, 2015-2017
- Lecturer, Stem Cell Biology, Stem Cells Seminars, University of Illinois at Chicago, 2014
- Supervisor, Summer Research Internships, UIC, 2008, 2017
- Training and Mentoring 12 UIC graduate and medical student from 2007-2016
Certification And Licensure
- NUCATS – CRC Basic Training Live, Northwestern University, Chicago, 2016
- GMP certification, University of Illinois at Chicago, 2009
- GLP certification, University of Illinois at Chicago, 2007
- Italian State medical licensure (MD), University of Pisa, 2003
- Hungarian State medical licensure (MD), University of Szeged, 2001
- Paramedic/EMO licensure, University of Szeged, 1999
Honors And Awards
- American Transplant Congress 2016, Poster of Distinction
- UIC Stem Cell and Regenerative Medicine Symposium 2012, Best Abstract Award
- Alessandro Bocelli Doctoral Fellowship Award 2002
- MD thesis - Excellence Award 2000
Grants And Support
- UIC Chancellor Intramural Research Award - Duchene Muscular Dystrophy, senior scientist, 2016
- Novel Tolerogenic CD34 MSC DiChimeric Cell Therapy in Vascularized Composite Allograft and Heart Transplant, AHA, Siemionow, 2016, 2017, key personnel
- Human Hematopoietic Cells Therapy for Transplantation Tolerance, CTA-1, CDMRP-DOD, 2015, 2017, senior scientist
- MSC in tolerance induction in macaques, NIH, Bartholomew, 2011, 2015, study director (effort 60%)
- Total Body Irradiation LD Curve in minipigs, BARDA, Bartholomew, 2014, 2015, prosector, surgical procedures (effort 30%)
- PTH as radiomitigator, BARDA, Bartholomew, 2008, 2010, study director (effort 60%)
- Total Body Irradiation LD Curve in macaques, BARDA, Bartholomew, 2007, 2019, prosector (effort 10%)
Patents And Technology Transfer
61/794,080, Activated mesenchymal stem cells for the prevention and repair of inflammatory states, US
Editorial Reviewer Activity
Cytotherapy, 2015
Languages
English
Italian
Hungarian
Spanish
Oral Presentations
- Stem Cell and Regenerative Medicine Symposium, UIC, Chicago, 2010, Activated mesenchymal stem cells synergize with Integra scaffold to promote increased wound VEGF and blood flow in non-human primates
- Workshop of Mesenchymal Stem Cells, Florence, Italy, 2004, Mesenchymal Stem Cells in Pancreatic Islet Transplantation
- European Tissue Repair Society 16th annual meeting, Pisa, Italy, 2006, 18, Immunomodulatory effect of mesenchymal stem cells in an experimental organ transplantation
Selected Posters
- Szilagyi, Erzsebet, Joanna Cwykiel, Anna Domaszewska-Szostek, and Maria Siemionow. "2550: Chimeric cell transplant for the treatment of duchenne muscular dystrophy." Vascularized Composite Allotransplantation 3, no. 1-2 (2016): 40-40.
- American Transplant Congress, 2016, Poster of Distinction, Chimeric Cell Transplant for Treatment of Duchenne Muscular Dystrophy: a Preliminary Report
- Connect Conference, 2016, Chimeric Cell Transplant for Treatment of Duchenne Muscular Dystrophy
- Khatibi, Katherine G., Erzsebet Szilagyi, Rachana Patil, Kevin Choi, and Amelia M. Bartholomew. "The Role of MHC Class II in Interferon Gamma-Activated Mesenchymal Stem Cell-Macrophage Crosstalk." Journal of the American College of Surgeons 221, no. 4 (2015): S159.
- American Transplant Congress, 2015, CMV prevalence in Mauritian and Indonesian Cynomolgus Macaques: Significance in Transplantation and CMV Disease Models, Am J Transplant, 15, suppl 3
- WTC 2014, Radiation Research Society Meeting, 2014, Mesenchymal Stem Cells Reduce Lung Injury and prevent Infection-related Mortality Following Irradiation
- American Transplant Congress, 2010, Reprogramming mesenchymal stem cells for increased tolerogenic capacity
- European Association for Study of Diabetes, Coppenhagen, Denmark, 2006, 0444, Mesenchymal stem cells modulate allogenic response in pancreatic islet transplantation
- 9th Symposium of Basic Science for Better Transplants, Nantes, France, 2005, 37, Mesenchymal stem cells modulate allogenic immune response in pancreatic islet transplantation
- Innovative Therapeutics in Transplantation, Nantes, France, 2006, 123, Immunosuppressive effect of mesenchymal stem cells in an experimental model of organ transplantation
Publications
https://www.ncbi.nlm.nih.gov/myncbi/1dg6xddwv0HAa/bibliography/public/
1. Malik M, Siemionow M, Cwykiel J, Heydemann A, Garcia-Martinez J, Siemionow K, Szilagyi E. Intraosseous transplant of dystrophin expressing chimeric (DEC) cells improves skeletal muscle function in mdx mouse model of Duchenne muscular dystrophy.
Postepy Kardiol Interwencyjnej. 2022 Dec;18(4):399-406.
2. Siemionow M, Szilagyi E, Cwykiel J, Domaszewska-Szostek A, Heydemann A, Garcia-Martinez J, Siemionow K. Transplantation of Dystrophin Expressing Chimeric Human Cells of Myoblast/Mesenchymal Stem Cell Origin Improves Function in Duchenne Muscular Dystrophy Model. Stem Cells Dev. 2021 Feb;30(4):190-202.
3. Carbonero F, Mayta A, Bolea M, Yu JZ, Lindeblad M, Lyubimov A, Neri F, Szilagyi E, Smith B, Halliday L, Bartholomew A. Specific Members of the Gut Microbiota are Reliable Biomarkers of Irradiation Intensity and Lethality in Large Animal Models of Human Health. Radiat Res. 2019 Jan;191(1):107-121.
4. Carbonero F, Mayta-Apaza AC, Yu JZ, Lindeblad M, Lyubimov A, Neri F, Szilagyi E, Bartholomew A. A comparative analysis of gut microbiota disturbances in the Gottingen minipig and rhesus macaque models of acute radiation syndrome following bioequivalent radiation exposures. Radiat Environ Biophys. 2018 Nov;57(4):419-426.
5. Siemionow M, Cwykiel J, Heydemann A, Garcia J, Marchese E, Siemionow K, Szilagyi E. Dystrophin Expressing Chimeric (DEC) Human Cells Provide a Potential Therapy for Duchenne Muscular Dystrophy. Stem Cell Rev Rep. 2018 Jun;14(3):370-384.
6. Siemionow M, Cwykiel J, Heydemann A, Garcia-Martinez J, Siemionow K, Szilagyi E. Creation of Dystrophin Expressing Chimeric Cells of Myoblast Origin as a Novel Stem Cell Based Therapy for Duchenne Muscular Dystrophy. Stem Cell Rev Rep. 2018 Apr;14(2):189-199.
7. Yu JZ, Lindeblad M, Lyubimov A, Neri F, Smith B, Szilagyi E, Halliday L, MacVittie T, Nanda J, Bartholomew A. Response to the "Letter to the Editor" by Dant, Stricklin and Millage on our Article: "Subject-Based versus Population- Based Care after Radiation Exposure". Radiat Res. 2016 Sep;186(3):323.
10. Lee S, Szilagyi E, Chen L, Premanand K, Dipietro LA, Ennis W, Bartholomew AM. Activated mesenchymal stem cells increase wound tensile strength in aged mouse model via macrophages. J Surg Res. 2012 May 29.
11. Longoni B, Szilàgyi E, Puviani L, Mazzanti B, Tripodi S, Caselli C, Paoli G, Urbani S, Quaranta P, Antonini S, Cintorino M, Saccardi R, Nardo B, Mosca F. Mesenchymal stem cell-based immunomodulation in allogeneic heterotopic heart-lung transplantation. Journal of Transplantation Technologies & Research. 2011
12. Szilagyi E, Mahmud N, and Bartholomew A. The Importance of Non-Human Primate Models for pre-clinical studies in Hematopoiesis. Allogenic Stem Cell Transplantation 2010 Contemporary Hematology, 2010, Book chapter, 767-787
13. Longoni B, Szilagyi E, Quaranta P, Paoli GT, Tripodi S, Urbani S, Mazzanti B, Rossi B, Fanci R, Demontis GC, Marzola P, Saccardi R, Cintorino M, Mosca F. Mesenchymal stem cells prevent acute rejection and prolong graft function in pancreatic islet transplantation. Diabetes Technol Ther. 2010 Jun; 12(6):435-46.
14. Marzola P, Longoni B, Szilagyi E, Merigo F, Nicolato E, Fiorini S, Paoli GT, Benati D, Mosca F, Sbarbati A. In vivo visualization of transplanted pancreatic islets by MRI: comparison between in vivo, histological and electron microscopy findings. Contrast Media Mol Imaging. 2009 May-Jun;4(3):13542
15. Bartholomew A, Polchert D, Szilagyi E, Douglas GW, Kenyon N. Mesenchymes stem cells in the induction of transplantation tolerance. Transplantation. 2009 May 15; 87(9 Suppl): S55-7. Review.
Timeline
Research Assistant Professor
University Of Illinois At Chicago
2015.01 - 2017.07
Visiting Research Assistant Professor
University of Illinois at Chicago
2011.01 - 2015.04
Postdoctoral Research Fellow (joint)
University of Illinois at Chicago
2009.01 - 2010.12
Postdoctoral Research Fellow
University of Illinois at Chicago
2007.06 - 2011.01
Postdoctoral Research Fellow
University of Pisa
2006.01 - 2007.01
Ph.D. - Transplant Immunology, Surgery And Biotechnology
University of Pisa
M.D. -
Albert Szent-Gyorgyi Medical School
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