Ethan E. Nguyen
Ballwin,MO
Summary
Skilled at providing exceptional customer service, supporting sales needs, and merchandising products. Excellent teamwork and leadership strengths. Offering readiness to learn and develop in fast-paced environment and a strong background in cash handling and customer service.
Overview
7
7
years of professional experience
Work History
Teacher Assistant/Instructor of Anatomy in Cadaver Lab
Truman State University
08.2024 - Current
- Student instructor of muscle, organ, bone, and other human anatomical features
- Experienced in memorization of complex anatomical features
- Cultivated effective communication skills and experience with teaching techniques
- Responsible for technical care and preservation of human cadavers, with extensive knowledge of the protocols involved
Volunteer Research Assistant
A.T. Still University
05.2023 - Current
- Working in the laboratory of Dr
- Priscilla Phillips; contributing to a variety of research projects centered around the bacterial pathogen Porphyromonas gingivalis (PG-W83)
- Gained experience in computer-based data collection and analysis of mass spectrometry data to identify significant protein changes within mutant PG-W83 strains
- Was trained in employing aseptic laboratory techniques and cultivating facultative and obligate anaerobes
- Observed or assisted in a variety of experiments using molecular biology techniques to generate and validate PG-W83 mutants
- Observed or assisted in a variety of experiments using culture-based and molecular biology-based approaches to investigate PG-W83 mutant vs
- Wildtype phenotypic characteristic in response to environmental changes
- Some of my early contributions were acknowledged in a poster presentation at the 15th annual Interdisciplinary Biomedical Research Symposium (IBRS) on Nov 11, 2023, at ATSU entitled 'Growth, Survival, and Persistence Characteristics of P
- Gingivalis W83'
- Later work will be presented at the 16th annual IBRS meeting in November 2024 in Kirksville and at the American Society of Microbiology ASM Microbe June 2025 meeting in Los Angeles
Clinician Researcher Development Program (CRDP) Internship
A.T. Still Research Institute
05.2024 - 08.2024
- Paid internship under the principal investigator Dr
- Priscilla Phillips
- Lead participant in the investigation of bacterial persistence mechanisms of the pathogen Porphyromonas gingivalis (PG W83)
- Conducted bacterial growth curve and survival curve assays in context of sublethal exposure to tetracycline and metronidazole antibiotics using both optical density (OD550) and viable colony formation units (CFU) approaches
- Prepared lyophilized concentrated culture filtrate (CCF), based on the hypothesis that bacteria secrete factors that regulate persister cell development in bacterial populations
- Developed protocols and optimized assays to quantify changes in the percentage of persister cells amongst PG W83 cultures, with or without CCF treatment, followed by an overnight exposure to sublethal dose of antibiotics of samples collected at select time-points during growth
- Gained experience in custom primer design for RT-qPCR analysis of PG RNA targets putatively involved in bacterial persistence based on published studies and using DNA, RNA, and protein databases and bioinformatic online tools
- The selected PG targets were identified by sequence homology to genes identified in other bacteria (e.g., E
- Coli, Vibrio sp., Staphylococcus sp.) reported to regulate persistence mechanisms (stringent response, SOS response) or associated mechanisms (e.g., quorum sensing, NO stress, ROS stress)
- Gained experience in data analysis, creating figures and tables, interpreting experimental outcomes, and generating reports for future dissemination in presentations and publications
University Tour Guide
Truman State University
01.2022 - 01.2024
- Company Overview: (Admissions Office)
- Provided personalized tours of Truman State campus
- Practiced communication skills orally, written, and technologically
- Assists in preparation of student recruitment events for Truman State
- (Admissions Office)
Volunteer Research Assistant
Truman State University
09.2022 - 05.2023
- Working in the laboratory of Dr
- Joyce Patrick on the study of gene function within Bacillus subtilis
- Investigated the yisR gene mutant that had been suggested to function within nutrient stressor response and sporulation mechanisms
- Created a knockout-bacterial mutant using PCR and bacteriophage transduction techniques to characterize the role of yisR in vitro
- Compared culture growth trends between mutant and wildtype strains under nutrient rich and limited conditions using optical density (OD550) and colony formation units (CFU)
- Applied and practiced concepts and techniques previously taught in biology and chemistry lab courses
- Supported previous hypothesis of yisR’s involvement in B
- Subtillis survival under nutrient-limited conditions
- My contributions were acknowledged in a recent seminar presented by Dr
- Patrick at ATSU’s Basic Science seminar series on Oct 6, 2023, entitled 'Biofilm formation by the plant-protective bacterium Bacillus subtilis in the presence of pesticides'
Independent Chemistry/Biology Tutor
Truman State University
01.2021 - 01.2022
- Experience in communicating difficult subject matters to multiple student clients
- Arranged for lesson plans that prepared students for rigorous course work
- Instructed courses included: General Chemistry, Organic Chemistry, General Biology, Statistics, and College Algebra
Co-creator
Subzero Dragons LLC.
01.2018 - 01.2022
- Company Overview: An ecommerce pet company focused on the shipping, cultivation, and sale of animals and dry goods nationally and into Canada
- Specialized in breeding and care of high-end Bearded Dragons (Pogona vitticeps) for specific genetic traits involved with hypomelanism, translucent morphs, witblit morphs, and zero morphs
- Organized individual sales on a day-to-day basis moving on average 15-20 animals a month
- Formatted and maintained multiple social media platforms and sales websites
- Gross estimated monthly income of 7 to 10,000 from live animal sales with additional income from dry goods and accessories
- Depending on the age of the animal, each retailed for around 500-800 per animal
- Successfully reared multiple clutches of Pogona vitticeps until maturity with extensive animal handling experience
- An ecommerce pet company focused on the shipping, cultivation, and sale of animals and dry goods nationally and into Canada
Customer Service Representative
Schnucks Markets Inc
01.2018 - 01.2020
- Worked for over 2 years at a local shopping center in customer service
- Utilized interpersonal skills regularly when assisting customers
Education
BS - Biology
Truman State University
Kirksville, MO05.2025
High School Graduate -
Parkway South Highschool
Ballwin, MO05.2021
Skills
- Strong work ethic
- Creative problem solver
- Extensive customer service skills and experience, both for a company and independently
- Adaptability
- Self-motivation
- Agarose gel electrophoresis
- SDS-PAGE gel electrophoresis
- Western blotting
- Southern blotting
- Light-field Microscopy
- Gram stain
- Endospore stain
- Acid-Fast Stain
- Negative stain
- Isolating microbial cultures
- Plating microbial cultures
- Freeze storing microbial cultures
- Bacterial planktonic culture
- Bacterial biofilm cell culture
- Bacteriophage transduction
- Dilution calculations
- Optical density calculations
- Constructing growth curves
- Quantitative Polymerase Chain Reaction (qPCR)
- Reverse Transcriptase Quantitative Polymerase Chain Reaction (RT-qPCR)
- Kerby-Bauer antibiotic susceptibility test
- Minimal Inhibitory Concentration (MIC) assay
- Crystal violet biofilm assay
- DNA sequence analysis
- DNA alignments
- In silico DNA analysis
- In silico amino acid sequence analysis
- NCBI bioinformatic tools
- BLAST
- Chimera 116 program
- Protein modeling
Extra-Curricular Activities
Delta Chi Fraternity, Kirksville, MO, 09/01/21, 11/01/23, Active Member, spent a cumulative 50 hours in volunteering for road-side cleanup and community outreach events.
Relevant Course Work
- Bacteriology and Immunology (BIOL 404)
- Biochemistry (CHEM 335)
- Pathophysiology (BIOL 353)
- Human Anatomy w/Lab (BIOL 365)
- General Microbiology w/ Lab (BIOL 304)
- Cellular Biology w/ Lab (BIOL 330)
- Genetics w/ lab (BIOL 300)
- Writing About Biology (BIOL 210)
- Organic Chemistry I & II w/ Lab (CHEM 329; CHEM 332)
- Statistics (STAT 190)
- Analytical Geometry and Calculus I (MATH 198)
Research Skills
- Agarose gel electrophoresis and gel extraction
- SDS-PAGE gel electrophoresis
- Western blotting
- Southern blotting
- Light-field Microscopy
- Gram stain
- Endospore stain
- Acid-Fast Stain
- Negative stain
- Isolating, plating, and freeze storing microbial cultures
- Bacterial planktonic and biofilm cell culture of an anaerobe and aerobic microbes
- Bacteriophage transduction in the creation of a mutant
- Dilution and optical density calculations
- Constructing a growth curve of a microbe under anaerobic conditions
- Quantitative Polymerase Chain Reaction (qPCR)
- Reverse Transcriptase Quantitative Polymerase Chain Reaction (RT-qPCR)
- Kerby-Bauer antibiotic susceptibility test
- Minimal Inhibitory Concentration (MIC) assay
- Crystal violet biofilm assay
- DNA sequence analysis and alignments
- In silico DNA and amino acid sequence analysis using NCBI bioinformatic tools such as BLAST
- Chimera 1.16 program for protein modeling
Awards
- National Key award, For activities as chair of Alumni Relations.
- Avid Program (Advancement Via Individual Determination), An in-school academic oriented program focused on sponsoring leadership and preparing students for future success.
Research
- RT-qPCR Analysis of CCF Treated P. gingivalis W83 Persistence-Related Genes, 11/16/24, Kirksville, MO, A.T. Still University
- Assay Development for the Study of Persistence in Porphyromonas gingivalis W83, 08/09/24, Kirksville, MO, A.T. Still University
Timeline
Teacher Assistant/Instructor of Anatomy in Cadaver Lab
Truman State University
08.2024 - Current
Clinician Researcher Development Program (CRDP) Internship
A.T. Still Research Institute
05.2024 - 08.2024
Volunteer Research Assistant
A.T. Still University
05.2023 - Current
Volunteer Research Assistant
Truman State University
09.2022 - 05.2023
University Tour Guide
Truman State University
01.2022 - 01.2024
Independent Chemistry/Biology Tutor
Truman State University
01.2021 - 01.2022
Co-creator
Subzero Dragons LLC.
01.2018 - 01.2022
Customer Service Representative
Schnucks Markets Inc
01.2018 - 01.2020
BS - Biology
Truman State University
High School Graduate -
Parkway South Highschool
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