Summary
Overview
Work History
Education
Skills
Software
Timeline
Publications
Fine woodworking
Associations
Publications
Fine woodworking
Generic

EUGENE MOORE, PhD

Assay Development

Summary

Accomplished thought leader with over 30 years of experience in assay development, innovation, and strategy across the fields of neuroscience, protein and peptide chemistry, HPLC, and molecular biology. Skilled in dissecting complex assay challenges, implementing quality control methods, and driving innovations to enhance workflow efficiency, reduce costs, and improving performance, robustness and reliability. Committed to leveraging cutting-edge technology to maintain a competitive edge in assay development. Motto: 'Make the impossible possible—if there's a way, it will be found.'

Overview

14
14
years of professional experience
9
9
years of post-secondary education

Work History

Director of Assay Development

AEGEA BIOTECHNOLOGIES, INC.
2 2023 - 8 2024
  • Recruited, developed and managed high-performing R&D team
  • Led initiative to design, test, and implement novel oligonucleotides for cancer diagnostics
  • P&L budget responsibility for R&D
  • Using a "design-to-chemistry" approach, successfully designed Switch-Blocker oligos that are fully multiplexable under universal cycling conditions
  • In collaboration with Argonaut Manufacturing Services, demonstrated multiplex reaction retained activity post-lyophilization with 2 ul lyodots and 20 ul lyobeads, simplifying reaction setup to just adding template and run. Room temperature stability significantly reduced storage and transport costs
  • In a separate proof-of-concept improved a mass spectrometry-based detection method from 10% MAF down to 0.0025% MAF, reduced cycling time by 50%, allowing client to run complete workflow in one day in 5-plex multiplex of KRAS G12D, KRAS G13D, EGFR T790M plus both wild-type alleles
  • Developed first-in-class oncology pre-commercial multiplexed panel for KRAS G12C, G12D, G13C, G13D, Q61H and A146T; EGFR Del19, L858R, T790M
  • First to demonstrate integrating a blocking oligo into NGS-library preparation, making Switch-Blocker compatible with targeted resequencing kits on market
  • Made Switch-Blocker oligos competitive against the de facto leaders in oligo design and synthesis through conception and development of custom bioinformatics pipeline. Resulting in increased first-synthesis success rate and competitive turnaround time
  • First phase automated generation of fully modified oligo construct candidates from simple input of COSMIC id
  • Phase 1 further generated optimal multiplex primer constructs so that all oligonucleotides are compatible in multiplex at assay inception
  • Reduced months of design work to approximately 15 minutes
  • Innovation was elevated to new heights in Phase 2 which successfully deployed trained and tested machine learning prediction models in a paradigm-changing first demonstration of identifying previously elusive and unknown structure-function correlations through pattern recognition within oligonucleotide base sequence and its modifications.

Sr. Scientist

BIOCEPT, INC.
08.2021 - 01.2023
  • Responsible for research, development and full analytical validation of next-generation sequencing-based diagnostics using ThermoFisher Oncomine Breast and Lung with an emphasis on characterization of leptomeningeal disease and other brain metastases
  • Problem solved and optimized the workflow for ctDNA isolation from cerebrospinal fluid (CSF) via deep-dive evaluation of commercially available collection tubes and automated nucleic acid extraction kits and measuring their impact on library formation for use on the Ion Torrent
  • Utilized SAMTools, IGV and other post-sequencing informatics and metrics tools to evaluate library and sequencing quality for low input libraries
  • By troubleshooting and optimizing each step of the process in a quality-by-design approach, found ideal solutions for previous deficits in assays originally designed for blood/plasma.

Scientific Associate

La Jolla Institute for Allergy and Immunology
11.2016 - 10.2019
  • Applyied expertise in protein chemistry to methods and process improvements of established MHC binding assays for Class I and Class II HLA epitopes
  • Developed a streamlined method for 125I radioisotopic labeling of peptides with enhanced HPLC QC that consistently provides higher, more pure yields of target radiolabel
  • Improved labeling and QC have resulted in considerable decrease in assay repeats and reduced overall consumption of costly reagents and consumables
  • Developed methods to expand the menu of Class I and II alleles in repertoire of binding assays to include co-expressed DRB3/4/5 and Class I HLA-C alleles through the creation of stably transfected cell lines
  • Responsibilities overlap into lab management roles such as negotiating for and procuring high ticket item lab supplies and equipment, training lab personnel in molecular biology, protein chemistry, and HPLC analytical methods
  • Collaborated with US and International research groups on immunology research projects

Co-Founder / Principal Scientist

Amplicon Diagnostics
01.2009 - 01.2016
  • Responsible for every aspect of the startup including methods development and assay validation, market analysis, marketing, raising capital, business development and cultivating vendor and business relationships
  • Actively sought funding through SBIR and I-Corps grants
  • Forged a working relationship with Veterinary Specialty Hospital (Sorrento Valley, CA) in the development of and validation of molecular diagnostic for detection of canine lymphoma and leukemia
  • Outside of this collaboration, additional efforts strived for the sensitive detection and stratification of other veterinary oncology diseases including hemangiosarcoma, and histiocytic sarcoma
  • Collected and tested blood and lymph node aspirates from canine patients across the United States and reported results in clinical setting
  • In 2016, pivoted to development of proprietary microfluidics-based real-time quantitative PCR-based point-of-care diagnostics platform with novel approach for enrichment of target blood cells prior to detection and quantification of pathological agents and various veterinary malignancies.

Education

Ph.D. - BIOCHEMISTRY AND MOLECULAR BIOLOGY

UNIVERSITY OF CALIFORNIA, RIVERSIDE
Riverside, CA
09.1997 - 04.2004

Bachelor of Science - MICROBIOLOGY

UNIVERSITY OF ARKANSAS, FAYETTEVILLE
Fayetteville, AR
09.1990 - 07.1993

Skills

Molecular Assay Development and Innovation

Software

Python

Timeline

Sr. Scientist

BIOCEPT, INC.
08.2021 - 01.2023

Scientific Associate

La Jolla Institute for Allergy and Immunology
11.2016 - 10.2019

Co-Founder / Principal Scientist

Amplicon Diagnostics
01.2009 - 01.2016

Ph.D. - BIOCHEMISTRY AND MOLECULAR BIOLOGY

UNIVERSITY OF CALIFORNIA, RIVERSIDE
09.1997 - 04.2004

Bachelor of Science - MICROBIOLOGY

UNIVERSITY OF ARKANSAS, FAYETTEVILLE
09.1990 - 07.1993

Director of Assay Development

AEGEA BIOTECHNOLOGIES, INC.
2 2023 - 8 2024

Publications

  • Developmentally distinct CD4+ Treg lineages shape the CD8+ T-cell response to acute Listeria infection, Joseph S. Dolina, Joey Lee, Eugene L. Moore, Jennifer L. Hope, Donald T. Gracias, Ashu Chawla, Jason A. Greenbaum, Stephen P. Schoenberger, PNAS, 119, 10, https://doi.org/10.1073/pnas.2113329119
  • Impact of Cysteine Residues on MHC Binding Predictions and Recognition by Tumor-Reactive T Cells, Abraham Sachs, Eugene Moore, Zeynep Kosaloglu-Yalcin, Bjoern Peters, John Sidney, Steven A. Rosenberg, Paul F. Robbins, Alessandro Sette, J Immunol, 205, 2, https://doi.org/10.4049/jimmunol.1901173
  • Sequence-based HLA-A, B, C, DP, DQ, and DR typing of 496 adults from San Diego, California, USA, Eugene Moore, Alba Grifoni, Daniela Weiskopf, Veronique Schulten, Cecilia S. Lindestam Arlehamn, Michael Angelo, John Pham, Shay Leary, John Sidney, David Broide, April Frazier, Elizabeth Phillips, Simon Mallal, Steven J. Mack, Alessandro Sette, Human Immunology, 79, 12, https://doi.org/10.1016/j.humimm.2018.09.008
  • Ampulexins: A new family of peptides in venom of the emerald jewel wasp, Ampulex compressa, Moore, E.L, Arvidson, R., Banks, C., Urenda, J.P., Duong, E., Mohammed, H., and Adams, M.E., Biochemistry, https://pubs.acs.org/doi/10.1021/acs.biochem.7b00916
  • Peptide-binding motifs of two common equine class I MHC molecules in Thoroughbred horses, Bergmann, T., Lindvall, M., Moore, E., Immunogenetics, 69, https://doi.org/10.1007/s00251-017-0978-6
  • Citrullination only infrequently impacts peptide binding to HLA class II MHC, John Sidney, Stephane Becart, Mimi Zhou, Karen Duffy, Mikaela Lindvall, Erin C. Moore, Eugene L. Moore, Tadimeti Rao, Navin Rao, Morten Nielsen, Bjoern Peters, Alessandro Sette, PLoS, http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0177140
  • Parasitoid Wasp Sting: A Cocktail of GABA, Taurine and β-alanine opens chloride channels for central synaptic block and transient paralysis of a cockroach host, Moore, E.L., Haspel, G., Libersat, F., and Adams, M.E., J. Neurobio, 66, http://onlinelibrary.wiley.com/doi/10.1002/neu.20254/abstract
  • Green Treefrogs and Grasshoppers as Vectors for Dispersal of Colletotrichum gloesporioides f. sp. Aeschynomene, Yang, X.B., Moore, E.L., and TeBeest, D.O., Proc. Ark. Acad. Sci.

Fine woodworking

Custom design one-off furniture and fine-art pieces with an avid fondness for craftsman style pieces.

Associations

  • American Chemical Society
  • Sons of the American Revolution (VP Membership)

Publications

  • Developmentally distinct CD4+ Treg lineages shape the CD8+ T-cell response to acute Listeria infection, Joseph S. Dolina, Joey Lee, Eugene L. Moore, Jennifer L. Hope, Donald T. Gracias, Ashu Chawla, Jason A. Greenbaum, Stephen P. Schoenberger, PNAS, 119, 10, https://doi.org/10.1073/pnas.2113329119
  • Impact of Cysteine Residues on MHC Binding Predictions and Recognition by Tumor-Reactive T Cells, Abraham Sachs, Eugene Moore, Zeynep Kosaloglu-Yalcin, Bjoern Peters, John Sidney, Steven A. Rosenberg, Paul F. Robbins, Alessandro Sette, J Immunol, 205, 2, https://doi.org/10.4049/jimmunol.1901173
  • Sequence-based HLA-A, B, C, DP, DQ, and DR typing of 496 adults from San Diego, California, USA, Eugene Moore, Alba Grifoni, Daniela Weiskopf, Veronique Schulten, Cecilia S. Lindestam Arlehamn, Michael Angelo, John Pham, Shay Leary, John Sidney, David Broide, April Frazier, Elizabeth Phillips, Simon Mallal, Steven J. Mack, Alessandro Sette, Human Immunology, 79, 12, https://doi.org/10.1016/j.humimm.2018.09.008
  • Ampulexins: A new family of peptides in venom of the emerald jewel wasp, Ampulex compressa, Moore, E.L, Arvidson, R., Banks, C., Urenda, J.P., Duong, E., Mohammed, H., and Adams, M.E., Biochemistry, https://pubs.acs.org/doi/10.1021/acs.biochem.7b00916
  • Peptide-binding motifs of two common equine class I MHC molecules in Thoroughbred horses, Bergmann, T., Lindvall, M., Moore, E., Immunogenetics, 69, https://doi.org/10.1007/s00251-017-0978-6
  • Citrullination only infrequently impacts peptide binding to HLA class II MHC, John Sidney, Stephane Becart, Mimi Zhou, Karen Duffy, Mikaela Lindvall, Erin C. Moore, Eugene L. Moore, Tadimeti Rao, Navin Rao, Morten Nielsen, Bjoern Peters, Alessandro Sette, PLoS, http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0177140
  • Parasitoid Wasp Sting: A Cocktail of GABA, Taurine and β-alanine opens chloride channels for central synaptic block and transient paralysis of a cockroach host, Moore, E.L., Haspel, G., Libersat, F., and Adams, M.E., J. Neurobio, 66, http://onlinelibrary.wiley.com/doi/10.1002/neu.20254/abstract
  • Green Treefrogs and Grasshoppers as Vectors for Dispersal of Colletotrichum gloesporioides f. sp. Aeschynomene, Yang, X.B., Moore, E.L., and TeBeest, D.O., Proc. Ark. Acad. Sci.

Fine woodworking

Custom design one-off furniture and fine-art pieces with an avid fondness for craftsman

EUGENE MOORE, PhDAssay Development