Fan Meng
Azusa,CA
Summary
Motivated and forward-thinking biochemist with over ten years of laboratory research experience in molecular biology, protein chemistry, and biomedical areas. Successfully oversaw large biomedical research projects, including designing, executing, analyzing, and validating molecular targets for therapeutic application and fundamental mechanism studies. Proficient in executing a variety of established biochemical and molecular biological techniques and strategies. Skilled in developing and optimizing experimental protocols and methods. Effective presenter and communicator with expertise in complex data analysis and interpretation. Demonstrated track record of peer-reviewed publications and presentations. Talented educator committed to teaching undergraduate chemistry and biochemistry courses. Articulate professional with substantial experience in assisting principle investigators (PIs) and supporting trainees/research staff. Skilled in curriculum development and assessment, with an understanding of different learning styles and abilities. Dedicated to creating a safe and inclusive working environment.
Overview
13
13
years of professional experience
2
2
Certification
Work History
Postdoctoral Fellow
CITY OF HOPE NATIONAL MEDICAL CENTER
10.2020 - Current
Lead and conduct all facets of assigned scientific research in the laboratory of Mustafa Raoof, including developing new experimental methods, processing in-vivo/in-vitro samples, large-scale genomic/proteomic analysis and image-based data analysis for complex biomedical research projects investigating transcription-replication conflict (TRC) role in pancreatic cancer
- lab management, graduate student training, and research operations’ coordination
- Responsible for the design, development and analysis of genomic – and proteomic – method to locate TRC’s location and regulatory proteins
- Applying high-throughput assay to characterize DNA damage repairing (DDR) pathway in TRC regulation in pancreatic cancer cell line
- Conducting in-vitro analysis to investigate the therapeutic effect and underlying molecular mechanism of small molecular compound targeting TRC in pancreatic cancer
- Investigating PCNA inhibitor, AOH, targeting PCNA, demonstrating the potential pharmaceutics benefits in kras-induced TRC pancreatic cancer
- Developing in-vitro proteomic method for locating protein members involving in regulating TRC formation/resolution, to identify potential therapeutic target for pancreatic cancer
Postdoctoral Scholar
UNIVERSITY OF CALIFORNIA, DIEGO, MEDICINE
10.2016 - 10.2020
Contributing to all facets of assigned research projects in the laboratory of Xiang-Dong Fu, including data collection, processing molecular sample and mechanistic studies with multidisciplinary experimental approaches
- Investigated Serine-Arginine protein kinas-1 (SRPK1)’s biochemical role(s) in pancreatic cancer cell line migration and chemotherapy resistance
- Instrumental in leading the mechanistic study on ALK inhibitor stimulating proliferation and metastasis of ALK (+) non-small cell lung cancer with EGF appearance
- Responsible for the designing and conducting experimental approach including in-vitro cell studies, gene level analysis RNA-sequencing, and protein binding analysis
- Collaborated with Guowei Wu (USCD) and Dr.Tinghu Zhang (Harvard University) in developing new generation small molecular inhibitors targeting on SRPK1, demonstrating the potential pharmaceutics benefits in anti-anagenesis aspect
- Coordinated with DrQian Hao (UCSD) in conducting in-vitro biochemical and cellular experiments for reprogramming isolated astrocytes into functional neurons by specific depletion of the RNA binding protein PTB in Parkinson model
- Supported Dr.Lan-Tao Gou (UCSD) in developing and executing in-vitro experiments and data analysis to investigate SRPK1’role in mediating initial genomic reprogramming and sequential activating fertilization
- Collaborated with Dr.Jin Hu (UCSD, CA) and Dr.Qian Hao (UCSD, CA) in planning and executing in-vitro biochemical experiments for complex mechanistic study on metabolic burst in cell reprogramming.
Graduate Research Assistant
MIAMI UNIVERSITY
09.2011 - 08.2016
Conducted bioinorganic and protein chemistry research by apply cutting-edge methods that contribute to the advancement biomedical and pharmaceutical science in disease research for the laboratory of Michael W Crowder.
- Oversaw and conducting biochemical experimental processes including as isolated protein model preparing, protein structure characterization, enzymatic kinetic studies, and structure-activity studies for developing small molecular compounds
- Led all experimental processes, research & data analysis, and manuscript writing for multiple projects on biochemical characterization and inhibitory studies on catalytic domain of MMP16/MT3-MMP, a tumorigenesis promoter in melanoma and NSCLC
- Spearheaded complete project cycle including experimental processes, cellular experiments, research, analysis, and reporting of biochemical characterization and inhibitory studies on catalytic domain of MMP7/ matrilysin-1, a vital driving factor in cancers and auto-inflammatory diseases, with novel ZBG inhibitors
- Utilized multiple statistical and biochemical models and tests to measure the ZBG strength of associations, that determined significant differences in drug binding mode by Structure Activity Relationships (SAR) approaching
- Effectively organized and communicated research results and planned activities by calculating and compiling raw data from large datasets, achieving publications of results peer-reviewed journals including JBIC and JIB Journals
Teaching Assistant
MIAMI UNIVERSITY
09.2011 - 05.2016
- Taught engaging courses in General Chemistry and Biochemistry for up to 50 diverse undergraduate students for large research university
- Delivered a range of teaching and assessment activities, including tutorials, seminars and lectures that contributed to the development of appropriate teaching materials and student learning objectives
- Evaluated student academic performance by developing and grading all assessments and assignments, providing effective, timely and appropriate feedback to students that supported their learning
- Contributed to the ongoing development and design of the Chemistry and Biomedical curriculum, in a manner that supports research and evidence-based approach to student learning
- Tracked and reported student performance and grades in all courses using multiple formats, including entering grade results in spreadsheets and on the online learning management system (LMS).
Education
Ph.D. - Biochemistry
08-2016
Dissertation: Biochemical characterization and zinc binding groups (ZBGs) inhibitory studies on the catalytic domain of matrix metalloproteinases (cdMMPs).
Bachelor of Science - Biochemistry
08-2011
Cum Laude
Skills
- Cancer Cell Lines Culturing, Passaging, and Engineering with CRISPER-CAS9 system
- Cell fraction techniques to divide cytoplasm and nucleus; Chromatin fraction technique to identify high-transcription region
- Multiple End-point Cell Viability/Proliferation/Apoptotic Assays (MTT, WTS, ATP Assays)
- Real-time Cell Proliferation Assay (RTCA) Real-time Cell Invasion/Migration Assay (RTCA-CIM)
- Multiple End-point Cell Migration/Invasion Assays (Trans-well, Wound-healing, Matrigel-invasion)
- Genotoxic assays( Comet assays, DCF assays, AP sites assay)
- DNA replication and cell cycle analysis( fiber analysis and synchronize-release assay)
- Immunofluorescence (IF) Con-focal microscopy on cell lines and patient samples, image processing and data analysis with ImageJ and QuPath
- Flow cytomtery, data collection/processing and analysis with FlowJo
- DNA Editing and cloningRNA Extraction and Purification
- Gel Electrophoresis of DNA and Protein Sample Immunostaining (western-blot, ELISA)
- SiRNA/ShRNA Transfection and Inducible Knock-down Cell Lines Preparing
- Polymerase Chain Reaction (PCR)/ Reverse Transcription (RT)- PCR/Quantitative PCR (qPCR)
- Protein Expression with Ecoli system, Purification with Fast protein liquid chromatography(FPLC)
- difficult-protein recovered and refolded from inclusion bodies(IBs)Homogeneous and Heterogeneous Metal-Substitution Techniques in Metalloprotein
- Protein-Protein interaction by proximity-ligation assay (PLA) and Co-immunoprecipitation(Co-IP)
- Enzyme Kinetic Study (Steady-state Kinetic and Pre-steady Kinetic Assay
- Sample preparation for library construction and deep genomic analysis with Chromatin immunoprecipitation sequencing (ChIP-seq), DNA-RNA immunoprecipitation sequencing (DRIP-seq) and RNA-seq
- Sample preparation for deep proteomic analysis from Isolation of proteion on nascent DNA(IPond) and Co-IP
- Matrix-assisted Laser Desorption/Ionization Mass Spectroscopy (MALDI-TOF)
- Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES)
- Ultraviolet–Visible Spectroscopy (UV-vis) & Circular Dichroism Spectroscopy (CD)
- Fluorescence emission spectroscopy
- 1H- Nuclear Magnetic Resonance Spectroscopy (1H-NMR)
- HPLC Chromatography & Isothermal Titration Calorimetry (ITC)
- X-ray Crystallography
- Extended X-ray Absorption Fine Structure Spectroscopy (EXAFS)
- Continuous Wave Electron Paramagnetic Resonance Spectroscopy (CW-EPR)
- Kinase assay for Screening Anti-Cancer Compounds (Z-lysate)
Certification
Translational Cancer Research for Basic Scientist, American Association for Cancer Research, 2024
Leadership and Teamwork Certification, University of California San Diego Extension, 2018
Accomplishments
Publications
1. Meng, F., Yang, H., Aitha, M., George, S., Tierney, D. L., and Crowder, M. W. (2016)” Biochemical and spectroscopic characterization of the catalytic domain of MMP16 (cdMMP16)”, JBIC Journal of Biological Inorganic Chemistry, 1-13.
2. Meng, F. Yang, H., Colin, J.,Zhang, H.Q.,Moller, A., Spivey,D., Page, R., Tierney,D.L., and Crowder,M.W. (2016) “Biochemical characterization and zinc binding groups (ZBGs) inhibitory studies on the catalytic domain of MMP7 (cdMMP7)” Journal of inorganic biochemistry 165: 7-17.
3. Hatcher, J., GuoWei, W, Chunyue, Z Jie,Z,. Meng, F,. et.al (2018) “SRPKIN-1: A Covalent SRPK1/2 Inhibitor that Potently Converts VEGF from Pro-angioenic to Anti-angiogenic Isoform” , Cell Press Chem Bio 25(4): 460-470.
4. Gou L-T., Lim.D.,…. Zhang X. , Meng. F.,Li H-R.,.et.al (2020) “Initiation of Parental Genome Reprogramming in Fertilized Oocyte by Splicing Kinase SRPK1-Catalyzed Protamine Phosphorylation”, Cell 180(6):1212-1227.
5. Qian, H., Kang, X., Hu, J., Zhang, D., Liang, Z., Meng, F., ... & Fu, X. D. (2020). Reversing a model of Parkinson’s disease with in situ converted nigral neurons. Nature, 582(7813), 550-556.
6. Hu, J , Hao. Q, Meng, F, et al (2019)“Transient Glycolytic Burst Preserves an Active Window of YAP to License Fibroblast Fate Switch to Induced Neuron” ( submitted to Nature Communication and peer reviewing ).
7. Meng, F., Liu, Y., & Raoof, M. (2022). YIA22-006: Characterization of Transcription-Replication Conflicts in KRAS-driven Pancreatic Cancer. Journal of the National Comprehensive Cancer Network, 20(3.5), YIA22-006..
8. Smith, S. J., *Meng, F., Lingeman, R., Li, C., Li, M., Boneh, G., ... & Raoof, M. (2023). Therapeutic Targeting of Oncogene-induced Transcription-Replication Conflicts in Pancreatic Ductal Adenocarcinoma. medRxiv, 2023-10. (submitted to Gastroenterology and peer reviewing, * listed as co-first author )
9. Bui, L. T., Cao, X., Wang, J. T., Meng, F., Feng, M., Arvanitis, L., ... & Raoof, M. (2023). Integrated characterization of cell types, states and molecular programs in disseminated appendiceal neoplasms. bioRxiv, 2023-09. (submitted to Nature Communication and peer reviewing,)
10. Meng, F., Li, T., Singh, A. K., Wang, Y., Attiyeh, M., Kohram, F., ... & Raoof, M. (2024). Base-excision repair pathway regulates transcription-replication conflicts in pancreatic ductal adenocarcinoma. Cell Reports, 43(10).
Conferences
1. Poster presentation. Characterization of matrix metalloproteinase, 246th ACS National Meeting & Exposition, Indianapolis, Indiana, 2013(Abstr. Pap. Am. Chem. Soc. 2013, 24).
2. Poster presentation, biochemical and spectroscopic characterization of the catalytic domain of MMP16 (cdMMP16), Graduate Research Forum, Miami University, Oxford, Ohio, 2016.
3. Poster presentation. Marc A. Attiyeh, Fan Meng, Yilun Liu, Nicholas Banovich, Mustafa Raoof. Pan-cancer analysis of promoter or promoter-proximal somatic mutations defines transcription-replication conflict mutational signature [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 6342.
4. Poster presentation, Fan Meng, Anup K. Singh, Tiane Li, Marc Attiyeh, Fatemeh Kohram, Terence Williams, Yilun Liu, Mustafa Raoof. Base excision repair pathway regulates transcription-replication conflicts in pancreatic ductal adenocarcinoma [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Pancreatic Cancer; 2023 Sep 27-30; Boston, Massachusetts. Philadelphia (PA): AACR; Cancer Res 2024;84(2 Suppl):Abstract nr C065.
5. Poster presentation.Fan Meng, Yilun Liu, Mustafa Raoof Neil2. Unexpected Transcription-Replication Conflicts(TRC) Regulator in Pancreatic Ductal Adenocarcinoma. DNA Damage, Mutation and Cancer, Gordon Research Conference , Ventura, California 2024
6. Poster presentation Fan Meng, Tian’e Li.,…. Yilun Liu, Mustafa Raoof. Base-excision repair pathway regulates transcription-replication conflicts in pancreatic ductal adenocarcinoma. 2024 SoCal Genome Stability Symposium, La Jolla, California 2024 (*Awarded with Third Prize in poster session).
Languages
Chinese (Mandarin)
Native or Bilingual
Timeline
Postdoctoral Fellow - CITY OF HOPE NATIONAL MEDICAL CENTER
10.2020 - Current
Postdoctoral Scholar - UNIVERSITY OF CALIFORNIA, DIEGO, MEDICINE
10.2016 - 10.2020
Graduate Research Assistant - MIAMI UNIVERSITY
09.2011 - 08.2016
Teaching Assistant - MIAMI UNIVERSITY
09.2011 - 05.2016
Translational Cancer Research for Basic Scientist, American Association for Cancer Research, 2024
Leadership and Teamwork Certification, University of California San Diego Extension, 2018
Miami University - Ph.D., Biochemistry
Ball State University - Bachelor of Science, Biochemistry
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