KRISHNA SONPAL
Union City
Summary
Aspiring Project Manager with a solid foundation in molecular biology. I am eager to transition into a project management role where I can leverage my analytical skills, organizational abilities, and passion for leadership. With a strong commitment to continuous learning and professional development, I am dedicated to mastering project management principles and methodologies. My goal is to contribute to successful project outcomes by ensuring efficient coordination, effective communication, and strategic planning.
Overview
12
12
years of professional experience
Work History
LIFE SCIENCE RESEARCH PROFESSIONAL
Stanford University
10.2022 - Current
- Led the coordination of single cell sequencing projects, overseeing the entire process from sample acquisition to data analysis, ensuring timely and accurate delivery of results
- Managed relationships with key stakeholders, providing expert customer support and training to enhance user experience and satisfaction
- Designed and implemented NGS experiments, aligning them with client goals and project timelines, resulting in optimized sequencing outcomes
- Troubleshot and resolved technical challenges in sequencing processes, streamlining operations and improving project efficiency
- Collaborated with cross-functional teams, facilitating effective communication and alignment across departments to achieve project objectives.
Assistant Specialist
University of California, San Francisco
12.2021 - 10.2022
- Led and managed the genome-wide perturb-seq screen project in primary T-cells, ensuring accurate execution and timely delivery of results
- Contributed to the development of XYZ-seq (spatial sequencing) technology, collaborating with cross-functional teams to drive innovation and project success.
Staff Research Associate II
University of California, San Francisco
07.2021 - 10.2021
- Oversaw genome editing experiments using the CRISPR system in human and other eukaryotic systems, facilitating GWAS CRISPR screen experiments
- Managed the maintenance and editing of human primary immune cells, ensuring the integrity and accuracy of experiments
- Coordinated the preparation of libraries for single-cell and bulk RNA sequencing (RNA-seq), open chromatin accessibility sequencing (ATAC-seq), and immune repertoire sequencing (TCR-seq), contributing to the success of research projects.
Senior Research Associate (Strain Engineering)
Antheia Inc, Menlo Park, CA
03.2020 - 04.2021
- Collaborated on a high-impact project to develop a yeast strain for producing pharmaceutical compounds, contributing to key milestones and final product development
- Led efforts in strain construction, improving final product titers and productivity through meticulous project planning and execution
- Developed and implemented experimental strategies, including high-throughput DNA construction using yeast homologous recombination, to support multiple ongoing projects
- Optimized growth and production conditions, ensuring the successful delivery of high-priority strains necessary to meet critical project deadlines
- Analyzed data, documented results, and communicated findings effectively through presentations and written reports, ensuring all stakeholders were informed of project progress.
Research Associate I
Translational Biosciences, Sunnyvale, CA
11.2018 - 03.2019
- Managed GLP-compliant aseptic techniques, including cell reviving, splitting, and freezing of various cancer cell lines, to support multiple research projects
- Screened cytotoxic drugs using cell-based assays, contributing to the advancement of cancer research
- Led data analysis efforts using GraphPad Prism, preparing lab reports, presentations, and writing Standard Operating Procedures to ensure research consistency and quality
- Oversaw inventory management, ensuring the availability of critical supplies and managing vendor relationships for cell line procurement.
Undergraduate Student Researcher
Mumbai University, India
08.2012 - 08.2018
- Conducted research on the UV protective activity of marigold extracts on yeast, utilizing chromatographic techniques to analyze the nutraceutical value of the extracts
- Designed and executed experiments to study the effect of UV light on plates with and without marigold extracts, contributing to the understanding of natural UV protection.
Graduate Researcher
California State University, Fresno, CA
01.2017 - 04.2018
- Investigated the stabilization of the inactive conformation of the CRP protein in E
- Coli, performing site-directed mutagenesis PCR to create and analyze successful protein mutants
- Engineered the E. Coli protein YdeH for mass production of c-di-GMP, executing a series of cloning, transformation, and protein purification tasks using advanced molecular biology techniques.
Education
Certificate in Project Management - Project Management
University of Washington
02.2025
Master of Biotechnology -
California State University, Fresno
08.2018
Bachelors in Biotechnology -
Mumbai University, India
01.2016
Skills
- Molecular Techniques: Next-Generation Sequencing (NGS) library preparation techniques, PCR, colony PCR (yeast and E coli), gel electrophoresis, DNA gel extraction, construct and primer design
- Animal tissue culture techniques: Trypsinization, thawing, passaging, freezing of cell lines, sub-culturing, cryopreservation and maintenance of cells
- Cloning (both homologous recombination-based - in vivo and in vitro, and ligase based), low and high throughput E coli and yeast transformations and minipreps, site directed mutagenesis, sequence analysis Strain integrations with the help of CRISPR-Cas9 system, SDS-PAGE, UV-VIS Spectrophotometry, Mass spectrometry, NMR, GC-MS Gas chromatography, HPLC, ELISA test, qPCR, Immunofluorescence, Immunocytochemistry, Western Blot, Protein extraction, Protein purification, protein expression (bacteria, mammalian cells), Cell based assay (MTS assay, MTT assay), Immunoassays ELISA, Immunoblot, etc, FACS (Flow Cytometry), Sanger sequencing
- Microbial Techniques: Media preparation, plating, staining, sub-culturing, streaking, extraction of metabolites, bacterial activation and inhibition assay, antibiotic susceptibility test
- Plant tissue culture techniques: Preparation of media, sterilization of explant, inoculation, sub-culturing and maintenance of explant
- Technical and Bioinformatics skills: DNA design software (Benchling), Molecule visualization software (PyMOL, RasMol), Spotfire, Modelling atomic structures (WinCoot), bioinformatics database and software, MS Office (Excel, Word, PowerPoint, etc)
Relevantexperienceandawards
- Lab technician intern at agriculture priority pollutants lab (2018)
- Teaching Laboratory Associate, California State University, Fresno (2016 - 2017)
- Faculty sponsored research award at CSU, Fresno (2017)
- FSPCA certified, preventive controls for human food training (2016)
- Marketing Intern, BLOOMIGO - Biotechnology career development centre (2015)
Timeline
LIFE SCIENCE RESEARCH PROFESSIONAL
Stanford University
10.2022 - Current
Assistant Specialist
University of California, San Francisco
12.2021 - 10.2022
Staff Research Associate II
University of California, San Francisco
07.2021 - 10.2021
Senior Research Associate (Strain Engineering)
Antheia Inc, Menlo Park, CA
03.2020 - 04.2021
Research Associate I
Translational Biosciences, Sunnyvale, CA
11.2018 - 03.2019
Graduate Researcher
California State University, Fresno, CA
01.2017 - 04.2018
Undergraduate Student Researcher
Mumbai University, India
08.2012 - 08.2018
Certificate in Project Management - Project Management
University of Washington
Master of Biotechnology -
California State University, Fresno
Bachelors in Biotechnology -
Mumbai University, India
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