Katelyn DeNegre
Vernon,CT
Summary
Driven by a passion for genetic research and a robust skill set in molecular biology techniques, I've made significant strides in understanding gene function at the University of Connecticut and The Jackson Laboratory. My expertise in reproductive and molecular biology and strong analytical abilities have propelled advancements in functional studies, showcasing my commitment to scientific excellence and collaborative success.
Overview
16
16
years of professional experience
Work History
PhD Research
University of Connecticut
Storrs, CT
08.2017 - Current
- Investigating the functional consequences of Xlr3 gene knockdown on mouse behavior, neurodevelopment, and male reproduction via transgenerational epigenetic inheritance
Research Assistant
The Jackson Laboratory
Bar Harbor, ME
10.2014 - 07.2017
- Analyzed mouse gene knockout tissues for LacZ expression as part of the LacZ screening aim of the Knockout Mouse Project (KOMP)
- Assessed mouse embryos for developmental defects as part of the embryonic lethal screening arm of KOMP
Undergraduate Student Researcher
University of Connecticut
Storrs, CT
01.2012 - 05.2014
- Assisted in making a short hairpin (shRNA) to knockdown Xlr3 mRNA and showed the knockdown to be successful in mouse fibroblast cell culture
- Explored allele-specific gene expression in Poeciliidae fish
Summer Student
The Jackson Laboratory
Bar Harbor, ME
06.2009 - 08.2009
- Independently assessed 2 Cre drivers for off target effects as part of The Jackson Laboratory Summer Student Program
Education
PhD - Molecular and Cell Biology
University of Connecticut
02.2025
BS - Physiology and Neurobiology
University of Connecticut
05.2014
Skills
- DNA/RNA extraction quantification, QC
- PCR and gel electrophoresis
- Sanger Sequencing and analysis
- cDNA synthesis
- Molecular cloning
- Transfection
- RT-PCR
- QRT-PCR
- Library preparation for NGS sequencing
- Oxford Nanopore Technologies DNA sequencing
- Illumina based RNA sequencing
- Adult and embryonic mouse necropsies
- Spermatocyte and oocyte cell surface spreads
- Cryo and paraffin sectioning
- Histology: IHC/ICC, Immunofluorescence, LacZ, H&E, PAS staining
- Microscopy: Brightfield and Fluorescence
- Mammalian cell culture
- Fluorescence Activated Cell Sorting
- Data entry to LIMS database
- Primer design
- ChIP-seq analysis
- RNA-seq analysis
- Oxford Nanopore Technology DNA methylation analysis
- Mouse Colony Management: breeding, timed matings, genotyping
- Programming languages: R, Linux, python
- Computer programs: GraphPad Prism, Microsoft Office
- Biological Databases: UCSC genome browser, BLAST, NCBI, GenBank, PANTHER, Gorilla, IGV, OMIM
Publications
Sobel Naveh, N., Foley, R.J., Denegre, K.R., Evans, T.C., Czechanski, A., Reinholdt, L.G., and O’Neill, M.J., Deficiency of SYCP3-related XLR3 disrupts the initiation of meiotic sex chromosome inactivation in mouse spermatogenesis., bioRxiv, 03/30/21, https://doi.org/10.1101/2021.03.30.437712
Presentations
- Oral Presentation, Functionally Characterizing SYCP3-Related XLR3 Involvement in Male Meiosis and Mouse Spermatogenesis, Society for the Study of Reproduction Annual Meeting, Dublin, Ireland, 07/24
- Poster Presentation, Transgenerational Epigenetic Inheritance and Autism Spectrum Disorder: Linking Meiotic Dysfunction and Cognitive Impairment, Society for the Study of Reproduction Annual Meeting, Dublin, Ireland, 07/24
- Poster Presentation, Blame Grandpa: Transgenerational Effects on Mouse Behavior Due to X Chromosome Epimutation, International Society for Autism Research Annual Meeting, Stockholm, Sweden, 05/23
Accomplishments
- Society for the Study of Reproduction Trainee Travel Award 2024
- University of Connecticut Graduate School Spring 2024 Demi Award 2024
- University of Connecticut Graduate School Conference Participation Award 2023
- Connecticut Institute for Brain and Cognitive Science (IBACS) Travel Award 2023
- Kenneth and Paula Munson Family Fund for Student Support in Health Sciences Fellowship 2023
- IBACS Graduate Student Summer Fellow 2021
- Outstanding Molecular and Cell Biology Teaching Assistant 2020-2021
- University of Connecticut Graduate Summer 2020 Demi Award
- Claire M. Berg Graduate Fellowship in Genetics 2019, 2023, 2024
- University of Connecticut Excellence in Teaching Award 2018-2020
- UCONN Summer Undergraduate Research Fellowship (SURF) 2013
Timeline
PhD Research
University of Connecticut
08.2017 - Current
Research Assistant
The Jackson Laboratory
10.2014 - 07.2017
Undergraduate Student Researcher
University of Connecticut
01.2012 - 05.2014
Summer Student
The Jackson Laboratory
06.2009 - 08.2009
PhD - Molecular and Cell Biology
University of Connecticut
BS - Physiology and Neurobiology
University of Connecticut
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