Summary
Overview
Work History
Education
Skills
Awarded Research Support
Teaching Mentoring Experience
Selected Publications
Certification
Timeline
Generic
Georgette Gafford

Georgette Gafford

Scottdale,GA

Summary

Dynamic researcher with extensive experience at Emory University, excelling in molecular techniques. Proven track record in grant writing and training diverse teams. Adept at lab management and safety compliance, fostering a collaborative environment to drive innovative research outcomes. Passionate about advancing scientific knowledge.

Overview

17
17
years of professional experience
7
7
Certification

Work History

Research Specialist Supervisor

Emory University
Atlanta, GA
10.2022 - Current
  • Processes blood to isolate peripheral blood mononuclear cells, bone marrow mononuclear cells and T cells for use in tissue culture or downstream experiments
  • Performs RNA extraction, qPCR, DNA extraction and western blot analysis
  • Manages mouse colony and conducts genotyping
  • Manages lab ordering, organization, IACUC protocol and lab safety requirements

Research Scientist

Emory University
Atlanta, GA
01.2021 - 09.2022
  • Conducted research related to Poldip2 protein as a mediator of cell permeability and inflammation using molecular and cell-based techniques.
  • Collaborated with a research team studying SARS Cov-2 effect on endothelial barrier and inflammation in a humanized mouse model. Evaluated potential therapeutics for lung and brain damage (BSL-3 research experience).
  • Protocol development and customization.
  • Trained cardiology and postdoctoral fellows, graduate and undergraduate students, and technicians in lab protocols and animal procedures.
  • Implemented and maintained lab safety requirements and conducts general lab management tasks.

Research Instructor

Emory University
Atlanta, GA
08.2015 - 01.2019
  • Conducted research into Fragile X Protein signaling in the mouse model
  • Collaborated with research team on behavioral and molecular characterization of the 3q29 deletion syndrome
  • Trained postdoctoral fellows in behavioral characterization and analysis
  • Developed and implemented radiation safety and lab safety requirements

Research Associate

Emory University
Atlanta, GA
04.2014 - 08.2015
  • Evaluated the role of neuropeptide signaling in learning and memory utilizing a wide variety of molecular techniques
  • Presented findings at national and international conferences
  • Wrote grants and received funding in a highly competitive funding environment
  • Advised undergraduate and graduate students in behavioral and molecular techniques
  • Developed and implemented best practices for lab organization and fulfillment of lab safety requirements

Postdoctoral Fellow

Emory University
Atlanta, GA
10.2008 - 04.2014
  • Evaluated the role of neuropeptide signaling in learning and memory utilizing a wide variety of molecular techniques
  • Presented findings at national and international conferences
  • Wrote grants and received funding in a highly competitive funding environment
  • Advised undergraduate and graduate students in behavioral and molecular techniques
  • Developed and implemented best practices for lab organization and fulfillment of lab safety requirements

Education

Ph.D. - Experimental Psychology: Neuroscience

University of Wisconsin – Milwaukee
12-2008

B.A. - Psychology

University of Nevada, Las Vegas
05.1999

Skills

  • DNA, RNA, protein extraction
  • ELISA
  • Western Blot
  • PCR
  • Quantitative real time (RT-PCR)
  • Cell culture
  • Blood processing for cell culture
  • Biosafety level 2, 3 experience
  • Mouse and rat Cannula implantation
  • Animal brain Microdissection
  • Animal behavior experiment design and analysis
  • Animal behavior software (Freezeframe, Limelight, SR Lab, Cleversys software
  • Histological processing (Microtome, Cryostat)
  • In situ hybridization, Immunohistochemistry
  • Microscopy (Brightfield, fluorescent, confocal)
  • Image processing (Image J/FIJI, Photoshop, Simple PCI, Q Capture)
  • PowerPoint, Excel, Word, SPSS, REDCap, PRISM Graphpad
  • Lab supply inventory management
  • Purchasing and Budget management (LIMS)
  • Mouse colony management (MLIMS)
  • Institutional Animal Care and Use Committee protocol preparation
  • Radiation safety compliance
  • Environmental Health and Safety Office compliance management

Awarded Research Support

  • NRSA Individual Postdoctoral Fellowship, National Institute of Health, F32 MH 090785, 2010-2013
  • Center for Behavioral Neuroscience Postdoctoral Fellowship, National Science Foundation, IBN-987675, 2008-2010

Teaching Mentoring Experience

  • Mentor, SURE (Summer Undergraduate Research Experience) and ION (Institute on Neuroscience), Emory University, 2010-2015
  • Guest lecturer, Advanced Physiological Psychology, University of Wisconsin-Milwaukee, 2008
  • Teaching assistant, Personality Psychology, Physiological Psychology, University of Wisconsin-Milwaukee, 2004-2006
  • Lab Instructor, Advanced Physiological Psychology, University of Wisconsin-Milwaukee, 2005, 2006

Selected Publications

  • Hu, R, White, T, Qu, H, Zhang, Z, Brockman, M, Valdivia, A, Joseph, G, Gafford, G, Burton, S, Taylor, W.R., Levit, R, Bassit, L, Derdeyn, C.A, Lassègue, B, Griendling, K and Hernandes, M., 2025, Poldip2 deficiency attenuates disease severity in a mouse model of COVID-19, In Preparation
  • Ou, Z, Dolmatova, E, Mandavilli, R, Qu, H, Gafford, GM, White, T, Valdivia, A, Lassègue, B, Hernandes, M, and Griendling, K., 2022, Myeloid Poldip2 Contributes to the Development of Pulmonary Inflammation by Regulating Neutrophil Adhesion in a Murine Model of Acute Respiratory Distress Syndrome., J Am Heart Assoc., 05/17, 11, 10
  • Rutkowski TP, Purcell, RG, Pollak, R, Grewenow, S, Gafford GM, Malone, T., Khan, U, Schroeder, J., Epstein, M., Bassell, G., Warren S., Weinshenker D., Caspary T., Mulle J., 2021, Behavioral changes and growth deficits in a CRISPR engineered mouse model of the schizophrenia-associated 3q29 deletion., Mol Psychiatry., 26, 3, 772-783
  • McCullough KM, Daskalakis NP, Gafford G, Morrison FG, Ressler KJ., 2018, Cell-Type Specific Interrogation of CeA Drd2 Neurons to Identify Targets for Pharmacological Modulation of Fear Extinction., Transl Psychiatry., 8, 1, 164
  • Rutkowski TP, Schroeder, J., Gafford GM, Warren S., Weinshenker D., Caspary T., Mulle J., 2017, Unraveling the genetic architecture of copy number variants associated with schizophrenia and other neuropsychiatric disorders., J. Neurosci Res., 95, 5, 1144-1160
  • Gafford, G.M. and Ressler KJ, 2016, Mouse models of fear related disorders - cell-type specific manipulations within the amygdala., Neuroscience, 321, 108-120, invited review
  • Gafford, G.M. and Ressler KJ, 2015, GABA and NMDA Receptors in CRF Neurons have opposing effects in fear acquisition and anxiety in Central Amgydala vs. Bed Nucleus of the Stria Terminalis., Hormones and Behavior, 76, 136-142, invited review
  • Gafford GM, Jasnow AJ, Ressler KJ, 2014, Grin1 Receptor Deletion within CRF Neurons Enhances Fear Memory., PLoS One., 9, 10, e111009
  • Gafford GM, Parsons RG, Helmstetter FJ., 2013, Memory accuracy predicts hippocampal mTOR pathway activation following retrieval of contextual fear memory., Hippocampus., 23, 9, 842-847
  • Gafford GM, Guo JD, Flandreau EI, Hazra R, Rainnie DG, Ressler KJ., 2012, Cell-type specific deletion of GABA(A)α1 in corticotropin-releasing factor-containing neurons enhances anxiety and disrupts fear extinction., Proc Natl Acad Sci U S A., 10/02, 109, 40, 16330-16335
  • Gafford GM, Parsons RG, Helmstetter FJ., 2011, Consolidation and reconsolidation of contextual fear memory requires mammalian target of rapamycin-dependent translation in the dorsal hippocampus., Neuroscience., 05/19, 182, 98–104
  • Gafford. G.M
  • Parsons, R.G.
  • Helmstetter, F.J., 2006, Translational control via the mammalian target of rapamycin (mTOR) pathway is critical for the formation and stability of long term fear memory in amygdala neurons., The Journal of Neuroscience, 26, 12977-12983, Both authors contributed equally to this publication.
  • Gafford, G.M., Parsons, R.G., Helmstetter, F.J., 2005, Effects of post training hippocampal injections of midazolam on fear conditioning., Learning and Memory, 12, 573-578

Certification

1. Good Clinical Laboratory Practice, GxP training

2. EHSO Shipping and Transport of Biological Materials, CITI Program

3. FDA Inspections of GMP facilities: How to Be Inspection Ready, CITI Program

4. Export Compliance, CITI Program

5. Biosafety, EHSO, Emory University

6. Research Lab safety, EHSO, Emory University

7. Bloodborne Pathogens for Research, EHSO, Emory University

Timeline

Research Specialist Supervisor

Emory University
10.2022 - Current

Research Scientist

Emory University
01.2021 - 09.2022

Research Instructor

Emory University
08.2015 - 01.2019

Research Associate

Emory University
04.2014 - 08.2015

Postdoctoral Fellow

Emory University
10.2008 - 04.2014

Ph.D. - Experimental Psychology: Neuroscience

University of Wisconsin – Milwaukee

B.A. - Psychology

University of Nevada, Las Vegas