Halley Shukla
Bensalem,USA
Summary
Accomplished research scientist with a PhD in Environmental Health Sciences and 8+ years of experience in molecular biology and immunology. Specializes in target validation for antibody-drug conjugates (ADC), assay development, and investigating molecular mechanisms of novel therapeutics. Leads cross-functional teams, develops new methodologies, and optimizes cell-based assays for enhanced data accuracy and efficiency. Skilled in non-compartmental PK analysis, functional assays with PBMCs, and cell-based internalization studies. Published in high-impact journals and recognized for advancing therapeutic research. Strong communicator experienced in drafting technical reports for regulatory submissions and presenting data at national/international conferences. Expert at managing multiple projects and delivering high-quality scientific results within tight deadlines.
Overview
11
11
years of professional experience
1
1
Certification
Work History
Research Scientist II
Immunome
11.2020 - Current
- Spearheaded the development of a comprehensive target validation package, ensuring timely project completion and delivering critical data that enabled key decision-making for therapeutic development
- Drove innovation in assay development by enhancing cell-based assays, improving data acquisition efficiency
- Developed and executed PK/PD assays, facilitating biomarker discovery and therapeutic advancement, and conducted in vivo studies to assess mechanisms of action and drug efficacy
- Led PBMC-based functional studies, directly validating novel drug targets and supporting the progression of therapeutic candidates
- Acted as the primary expert for cell-based internalization assays, significantly contributing to the elucidation of mechanisms for novel ADC targets, which guided crucial drug development strategies
- Authored and reviewed technical sections for regulatory submissions, including IND filings, ensuring accuracy, adherence to industry standards, and alignment with regulatory guidelines
- Maintained precise and detailed documentation of scientific and regulatory processes, safeguarding data integrity and compliance with stringent regulatory requirements.
Postdoctoral T32 Fellow
Children’s Hospital of Philadelphia
11.2018 - 11.2020
- Independently designed and executed key experiments under the prestigious RO1 NIH grant HL 131872 and NIH/NHLBI 5T32 HL007915, contributing crucial insights into the role of serotonin in Aortic/Mitral valve disease patients
- Successfully collaborated with a researcher at Florida Atlantic University to conduct a groundbreaking mouse study utilizing the SERT Met 172 mutant, advancing the understanding of serotonin transport in cardiovascular disease
- Authored and implemented an IACUC-approved protocol for an in vivo study investigating the effects of antidepressants and aspirin on platelet activation in the SERT Met 172 mouse model, providing new data on drug interactions in cardiovascular health
- Led a complex platelet activation project using multi-color flow cytometry, delivering novel findings on the impact of SSRIs on platelet activation in mice, canines, and humans using an ex vivo system
- Demonstrated strategic project management by independently developing research goals, prioritizing multiple high-impact projects, and identifying critical resources to ensure timely completion
- Analyzed and accurately interpreted complex experimental results, presenting significant findings at national and international conferences, further establishing expertise in cardiovascular research.
Ph.D. Student
The University of North Carolina at Greensboro
08.2013 - 05.2018
- Investigated the impact of the air pollutant Benzo-(A)-pyrene-1,6-quinone on endothelial cell injury and dysfunction, uncovering its implications for chemical atherogenesis and contributing to our understanding of pollutant-induced cardiovascular risks
- Proposed a novel redox cycling pathway for the generation of reactive oxygen species by Benzo-(A)-pyrene-1,6-quinone in endothelial cells, advancing the field's knowledge of oxidative stress mechanisms in environmental toxicology
- Received the prestigious Robert R
- Bryden Graduate Award from the North Carolina Academy of Science in April 2017 for outstanding research contributions, with an honorable mention in April 2016, highlighting exceptional academic and research achievements
- Awarded the Graduate Student Research Award in May 2016, recognizing innovative research and significant contributions to the scientific community
- Mentored and supervised both undergraduate and graduate students on their research projects, actively contributing to their professional development and enhancing the quality of scientific research within the academic community.
Education
Ph.D. - Environmental Health Sciences
University of North Carolina Greensboro
Greensboro, NC08-2018
Master of Science - Biology
University of North Carolina Greensboro
Greensboro, NC08-2013
Skills
- RNA and DNA isolation
- Transfection assays
- RT2 profiling (Protein and gene expression profiling) and qRT-PCR
- Cytotoxicity assays
- FACS/ multicolor Flow cytometry
- ELISA assays
- Ex-vivo Chandler loop/ platelet assays
- Western Blotting- Traditional/ simple Wes
- Human BOEC cell culturing from blood
- Genotyping
- PK/PD analysis using PK solver
- Cell-based immunology assay with PBMCs
- Cell-based internalization and cytotoxicity assays
- Cell-based functional assays
- Functional assays with PBMC
- Primary cell culture
- Microsoft office
- Statistical analysis software GraphPad PRISM
- Establish new methods and protocols
- Expert in the interpretation and analysis of complex data
- Dotmatics- Electronic Lab notebook
- Flow Jo
Certification
- Drug Development, Coursera, 05/2019, No expiration date
- Introductory course in Immunology, American association of immunologists, 07/2021
Citizenship
US Citizen. No visa sponsorship is required
Presentations
- Society of Toxicology (SOT) 2013, and 2017
- North Carolina Academy of Science (NCAS) 2013, 2015, 2016 and 2017
- Graduate research expo at UNCG
Publications
- Decreased serotonin transporter activity in the mitral valve contributes to progression of degenerative mitral regurgitation, Castillero E, Fitzpatrick E, Keeney SJ, D'Angelo AM, Pressly BB, Simpson MT, Kurade M, Erwin WC, Moreno V, Camillo C, Shukla HJ, Inamdar VV, Aghali A, Grau JB, Salvati E, Nissim I, Rauova L, Oyama MA, Stachelek SJ, Brown C, Krieger AM, Levy RJ, Ferrari G, Sci Transl Med, 01/04/2023, 10.1126/scitranslmed.adc9606, 36599005
- IMM-BCP-01, a patient-derived anti-SARS-CoV-2 antibody cocktail, is active across variants of concern including Omicron BA.1 and BA.2, Nikitin PA, DiMuzio JM, Dowling JP, Patel NB, Bingaman-Steele JL, Heimbach BC, Henriquez N, Nicolescu C, Polley A, Sikorski EL, Howanski RJ, Nath M, Shukla H, Scheaffer SM, Finn JP, Liang LF, Smith T, Storm N, McKay LGA, Johnson RI, Malsick LE, Honko AN, Griffiths A, Diamond MS, Sarma P, Geising DH, Morin MJ, Robinson MK, Sci Immunol, 09/09/2022, 10.1126/sciimmunol.abl9943, 35771946, PMC9273042
- Protection of HepG2 cells against acrolein toxicity by 2-cyano-3,12-dioxooleana-1,9 dien-28-imidazolide via glutathione-mediated mechanism, Shah H, Speen AM, Saunders C, Brooke EA, Nallasamy P, Zhu H, Li YR, Jia Z, Exp Biol Med (Maywood), 10/01/2015, 10.1177/1535370214563900
- Mechanisms of CDDO-imidazolide-mediated cytoprotection against acrolein-induced neurocytotoxicity in SH-SY5Y cells and primary human astrocytes, Speen A, Jones C, Patel R, Shah H, Nallasamy P, Brooke EA, Zhu H, Li YR, Jia Z, Toxicol Lett, 10/01/2015, 10.1016/j.toxlet.2015.07.005
- Luteolin protects against vascular inflammation in mice and TNF-alpha-induced monocyte adhesion to endothelial cells via suppressing IΚBα/NF-κB signaling pathway, Jia Z, Nallasamy P, Liu D, Shah H, Li JZ, Chitrakar R, Si H, McCormick J, Zhu H, Zhen W, Li Y, J Nutr Biochem, 03/01/2015, 10.1016/j.jnutbio.2014.11.008
- Sulforaphane reduces vascular inflammation in mice and prevents TNF-α-induced monocyte adhesion to primary endothelial cells through interfering with the NF-κB pathway, Nallasamy P, Si H, Babu PV, Pan D, Fu Y, Brooke EA, Shah H, Zhen W, Zhu H, Liu D, Li Y, Jia Z, J Nutr Biochem, 08/01/2014, 10.1016/j.jnutbio.2014.03.011
- Reactive oxygen species production by BP-1,6-quinone and its effects on the endothelial dysfunction: Involvement of the mitochondria, Halley Shukla, Rojin Chitrakar, Humaira A. Bibi, Gabriella Gaje, Ashkon Koucheki, Michael A Trush, Hong Zhu, Y. Robert Li, Zhenquan Jia, Toxicology Lett, 01/2020, https://doi.org/10.1016/j.toxlet.2020.01.011
- Targeting glutathione with the triterpenoid CDDO-Im protects against benzo-a-pyrene-1,6-quinone-induced cytotoxicity in endothelial cells, Halley Shukla, Ho Young Lee, Ashkon Koucheki Humaira A Bibi, Gabriella Gaje, Xiaolun Sun, Hong Zhu, Y Robert Li, Zhenquan Jia, Mol Cell Biochem, 07/27/2020, 10.1007/s11010-020-03831-6
Timeline
Research Scientist II
Immunome
11.2020 - Current
Postdoctoral T32 Fellow
Children’s Hospital of Philadelphia
11.2018 - 11.2020
Ph.D. Student
The University of North Carolina at Greensboro
08.2013 - 05.2018
Ph.D. - Environmental Health Sciences
University of North Carolina Greensboro
Master of Science - Biology
University of North Carolina Greensboro
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