Hilario Romero Mora

• Participated in routine maintenance schedules for all laboratory instruments, reducing downtime due to malfunctions or calibration issues.
• Managed inventory of laboratory supplies, ensuring availability of necessary materials while minimizing waste and overstocking costs.
• Picking, inoculating, and harvesting of plasmid DNA templates grown through e. coli
• Purify harvested plasmid DNA through columns and use of buffers
• Analyze purity of plasmids through the use of spectrophotometer and UV
• Further analyze plasmid DNA with digest restriction, gel electrophoresis, and sequencing
• Automate processing through the use of liquid handlers

• Read and follow established SOP’s, document activities and information using GDP
• Adhere to gowning procedures required to work in cleanroom environment
• Review inventory and maintain adequate supplies in GMP stock room, clean, and stage materials for delivery into cleanrooms
• Follow BPR to prepare buffer and media as well as manage collection and disposal of solid/ liquid waste from manufacturing
• Manufacture gene and cell therapy products for use in clinical setting
• Use proper aseptic techniques to ensure sterility of gene and cell therapy products

Perform manufacturing process in a sanitized laboratory setting with routine use of:

• Biological Safety Cabinets (BSC),
• Incubators,
• Micropipettes (P200-P1000),
• NC-200,
• Climet,
• Surface Air System (SAS),
• Controlled Rate Freezer (CRF),
• Environment and Personal Monitoring to ensure sterility and no growth of microorganisms.

• Synthesize oligonucleotides by following established SOP's
• Operate complex robotic systems to manage liquid product through a highly automated workflow
• Hamilton Automated Liquid Handler, TECAN Automated Liquid Handler

Process oligonucleotides through various purification processes:

• Solid-Phase Extraction (SPE),
• Size-exclusion Chromatography (SEC),
• Electrospray Ion Source (ESI) for mass spectrometric analysis
• Oligonucleotide bulking and plating of sgRNA and multi-guide sgRNA

• Abide by established SOPs and help improve current processes
• Prepare media and reagents for use on Liquid Handler machines.