HYO MIN KIM
Boston,MA
Summary
Research professional with extensive experience at Boston Children’s Hospital, specializing in in vitro and in vivo studies. Expertise in cell culture and rodent study techniques, contributing to innovative projects with strong analytical skills. Demonstrated ability to achieve results in high-pressure environments.
Overview
4
4
years of professional experience
Work History
Research Assistant
Boston Children’s Hospital
Boston, MA
07.2023 - Current
- Conducted research on adaptor protein 4-related hereditary spastic paraplegia (AP-4 HSP).
- Performed in vitro experiments with SH-SY5Y neuroblastoma cell line using CRISPR and small molecule screening.
- Executed in vivo studies on AP4B1 deficient mice to evaluate drug candidates from in vitro findings.
- Utilized intraperitoneal injection for efficient substance administration in animal models.
Undergraduate Researcher
Boston University
Boston, MA
09.2021 - 05.2023
- Investigated production of K4 phosphate, an angiotensin-converting enzyme inhibitor.
- Truncated K4-producing plasmid into 23 segments for conjugation with K4 nonproducing plasmid.
- Administered resultant plasmid to streptomyces bacteria and cultivated on agar plates.
- Isolated and analyzed synthesized products using LC/MS for each strain.
- Collaborated with Vegas lab to explore small molecule inhibitors of Interleukin-4.
- Cultivated HEK-blue cell line for cell-based HTS assays and differential scanning fluorimetry.
- Maintained THP-1 cell line for additional investigations.
Education
Bachelor of Science - Biology
Boston University
Boston, MA05.2023
Some College (No Degree) -
Georgia State University – Perimeter College
Dunwoody, GASkills
- Chromatography-based purification
- SDS-PAGE analysis
- DNA gel electrophoresis
- Protein overexpression in E coli
- Site-directed mutagenesis
- Subcloning techniques
- Plasmid preparation
- Enzymatic assays
- NMR characterization
- Conjugation processes
- Streptomyces maintenance
- Cell culture techniques
- High-throughput screening assays
- High-throughput microscopy
- Western blotting
- Differential scanning fluorimetry
- Immunocytochemistry techniques
- Immunohistochemistry techniques
- Rodent studies
- Perfusion methods
- Tissue dissection techniques
- Python programming
- Microsoft Excel proficiency
Publications
- Hu, W.-Y., Li, K., Weitz, A., Wen, A., Kim, H., Murray, J. C., Cheng, R., Chen, B., Naowarojna, N., Grinstaff, M. W., Elliott, S. J., Chen, J.-S., & Liu, P., Light-driven oxidative demethylation reaction catalyzed by a Rieske-type non-heme iron enzyme STC2., ACS Catalysis, 12, 23, 14559–14570, 11/23/22, https://doi.org/10.1021/acscatal.2c04232
- Paris, J., Wen, A., Hu, S., Weitz, A., Kim, H., Cheng, R., Elliott, S., Liu, P., and Guo, Y., Sulfoxide Synthase OvoAMtht Uses an Unprecedented S = 1 Ferryl Intermediate to Enable a C-S Bond Formation Reaction in Ovothiol Biosynthesis., Science, submitted
- Quiroz, V., Planas-Serra, L., Sveden, A., Tam, A., Kim, H.-M., Zubair, U., Resch, D., Saffari, A., Danzi, M. C., Züchner, S., Chopra, M., Schierbaum, L., Pujol, A., Eklund, E. A., & Ebrahimi-Fakhari, D., Biallelic variants in RINT1 present as early-onset pure hereditary spastic paraplegia., The Journal of Clinical Investigation, 07/11/24, https://www.jci.org/articles/view/178919
- Rosengarten, H., D'Amore, A., Kim, H. M., & Ebrahimi-Fakhari, D., ap4b1 -/- zebrafish demonstrate morphological and motor abnormalities., Human molecular genetics, Advance online publication, 2025, https://doi.org/10.1093/hmg/ddaf056
Languages
English
Full Professional
Japanese
Professional
Korean
Native/ Bilingual
Timeline
Research Assistant
Boston Children’s Hospital
07.2023 - Current
Undergraduate Researcher
Boston University
09.2021 - 05.2023
Bachelor of Science - Biology
Boston University
Some College (No Degree) -
Georgia State University – Perimeter College
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