Italo Rodrigo Calori

Dr. Calori is a research scientist specializing in tissue engineering, with a focus on 3D printing and bioprinting, spheroids, vascularized tissue models, organ-on-a-chip, and other microfluidic platforms for high-throughput applications.

His expertise integrates chemistry, engineering, and pharmaceutical sciences to develop innovative experimental platforms for drug testing and reproductive biology research. Dr Calori`s work focuses on advancing New Approach Methods, including physiologically relevant in vitro models, and alternative testing strategies aimed at reducing animal use in biomedical research.

• 1. CALORI, I.R.; GUIMARAES, A.P.P.; KAMMALA, A.K.; RICHARDSON, L.S.; MENON, R. Beyond monolayers: a comparative analysis of 2D cell cultures and 3D in vitro models as new approach methodologies. Frontiers in Bioengineering and Biotechnology, 2026
• 2. PIVA, H.L.; GUSM, L.A.; CALORI, I.R.; MELO, M.T.; FERREIRA, G.A.; NEDER, L.; TEDESCO, A.C. Albumin-based cationic nanoemulsion for Protoporphyrin IX delivery in normoxic and hypoxic conditions. Next Nanotechnology. 2026
• 3. SILVESTRIN, A.G.; DA SILVA, A.C.P.; DE FREITAS, C.F.; CALORI, I.R.; et al. Liposomal co-formulation of Azure A and Rose Bengal decyl ester as a multicellular target approach for photodynamic therapy of colorectal cancer. Journal of Drug Delivery Science and Technology, 2026.
• 4. MUSHIMIYIMANA, I.; RICHARDSON, L.; HORVATH, T.D.; ORLOVSKY, V.; HARRINGTON, B.; CALORI, I.R.; KAMMALA, A.K.; MENON, R. Heat-induced pathophysiological and metabolic changes at the feto-maternal interface predisposing to preterm birth. The FASEB Journal, 2025.
• 5. CALORI, I.R.; GUIMARAES, A.P.P.; TEDESCO, A.C. 3D printing in theranostic applications. Advanced Drug Delivery Reviews, 2026.
• 6. KENDAL-WRIGHT, C.E.; MOORE, J.J.; FENG, L.; et al. A call to standardize the nomenclature of human fetal membrane at the feto-maternal interface. Placenta, 2025.
• 7. CALORI, I.R.; GUIMARAES, A.P.P.; TEDESCO, A.C. Spheroid-matrix kidney-on-a-chip model for predicting drug-induced nephrotoxicity. BioChip Journal, 2025.
• 8. MARTINS, T.J.; CALORI, I.R.; GUIMARAES, A.P.P.; RIBEIRO, G.H.; VENNCIO, T.; MARTINS, F.; RIBEIRO, L.S.; CAMARGO, E.; TEDESCO, A.C. Norepinephrine-loaded thermoresponsive hydrogel-like system for enhanced angiogenesis in tissue engineering. Journal of Drug Delivery Science and Technology, 2025.
• 9. GONCALVES, R.R.; DOS SANTOS, L.F.; GOMES, L.F.T.; LIMA, K.D.O.; DALOSSIO, V.P.; CALORI, I.R.; BORGES, H.S.; GUSM, L.A.; PIVA, H.L.; TEDESCO, A.C.; GOLDNER, P. Upconversion luminescent hollow Er3+, Yb3+ co-doped Y2SiO5 spheres for biophotonics: in vitro cytotoxicity and cellular sub-locality evaluation in glioblastoma cells. Materials Today Communications, 2024.
• 10. CALORI, I.R.; TEDESCO, A.C. How can nanoemulsions be used for photosensitizer drug delivery? Expert Opinion on Drug Delivery, 2024.
• 11. CALORI, I.R.; LAMPROU, D.A. 3D printing in pharmaceuticals and medical applications. Frontiers in Drug Delivery, 2024, 4: 1527225.
• 12. GUIMARAES, A.P.P.; CALORI, I.R.; BI, H.; TEDESCO, A.C. Spheromold: modernizing the hanging drop method for spheroid culture. Frontiers in Drug Delivery, 2024, 4: 1397153.
• 13. GUIMARAES, A.P.P.; CALORI, I.R.; STILHANO, R.S.; TEDESCO, A.C. Renal proximal tubule-on-a-chip in PDMS: fabrication, functionalization, and RPTEC:HUVEC co-culture evaluation. Biofabrication, 2024, 16(2): 025024.
• 14. TRIGO-GUTIERREZ, J.K.; CALORI, I.R.; BARBARA, G.O.; PAVARINA, A.C.; GONCALVES, R.S.; CAETANO, W.; TEDESCO, A.C.; MIMA, E.G.O. Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity. Frontiers in Microbiology, 2023.
• 15. ALVES, S.R.; CALORI, I.R.; BI, H.; TEDESCO, A.C. Characterization of glioblastoma spheroid models for drug screening and phototherapy assays. OpenNano, 2023.
• 16. CALORI, I.R.; ALVES, S.R.; BI, H.; TEDESCO, A.C. Type-I collagen/collagenase modulates the 3D structure and behavior of glioblastoma spheroid models. ACS Applied Bio Materials, 2022.
• 17. CALORI, I.R.; PINHEIRO, L.; BRAGA, G.; DE MORAIS, F.A.P.; CAETANO, W.; TEDESCO, A.C.; HIOKA, N. Interaction of triblock copolymers (Pluronics) with DMPC vesicles: a photophysical and computational study. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2022, 121178.
• 18. MONICO, D.A.; CALORI, I.R.; SOUZA, C.; ESPREAFICO, E.M.; BI, H.; TEDESCO, A.C. Melanoma spheroid-containing artificial dermis as an alternative approach to in vivo models. Experimental Cell Research, 2022, 113207.
• 19. CALORI, I.R.; BI, H.; TEDESCO, A.C. Expanding the limits of photodynamic therapy: the design of organelles and hypoxia-targeting nanomaterials for enhanced photokilling of cancer. ACS Applied Bio Materials, 2021, 4: 195-228.
• 20. DE OLIVEIRA, E.L.; FERREIRA, S.B.S.; DE CASTRO-HOSHINO, L.V.; CAMPANHOLI, K.S.S.; DE MORAIS, F.A.P.; KIMURA, E.; JUNIOR, R.C.S.; BRUSCHI, M.L.; SATO, F.; HIOKA, N.; CAETANO, W. Thermoresponsive hydrogel-loading aluminum chloride phthalocyanine as a drug release platform for topical administration in photodynamic therapy. Langmuir, 2021, 37: 3202-3213.
• 21. ALVES, S.R.; CALORI, I.R.; TEDESCO, A.C. Photosensitizer-based metal-organic frameworks for highly effective photodynamic therapy. Materials Science & Engineering C: Materials for Biological Applications, 2021.
• 22. CALORI, I.R.; GUSM, L.A.; TEDESCO, A.C. B6 vitamers as generators and scavengers of reactive oxygen species. Journal of Photochemistry and Photobiology, 2021, 100041.
• 23. CALORI, I.R.; CAETANO, W.; TEDESCO, A.C.; HIOKA, N. Determination of critical micelle temperature of Pluronics in Pluronic/gel phase liposome mixtures using steady-state anisotropy. Journal of Molecular Liquids, 2020, 304: 112784.
• 24. CALORI, I.R.; PIVA, H.L.; TEDESCO, A.C. Targeted cancer therapy using alpha-cyano-4-hydroxycinnamic acid as a novel vector molecule: a proof-of-concept study. Journal of Drug Delivery Science and Technology, 2020, 57: 101633.
• 25. CALORI, I.R.; BRAGA, G.; DE JESUS, P.C.C.; BI, H.; TEDESCO, A.C. Polymer scaffolds as drug delivery systems. European Polymer Journal, 2020, 129: 109621.
• 26. DE MORAIS, F.A.P.; SONCHINI, R.G.; BRAGA, G.; PEREIRA, P.C.S.; BATISTELA, V.R.; CAETANO, W.; HIOKA, N. Stable dipalmitoylphosphatidylcholine liposomes coated with an F127 copolymer for hypericin loading and delivery. ACS Applied Nano Materials, 2020, 3: 4530-4541.
• 27. CALORI, I.R.; CAETANO, W.; TEDESCO, A.C.; HIOKA, N. Self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study. Dyes and Pigments, 2020, 182: 108598.
• 28. DE OLIVEIRA, D.C.S.; DE FREITAS, C.F.; GONCALVES, R.S.; CARDINALI, C.A.E.F.; MALACARNE, L.C.; RAVANELLI, M.I.; DE OLIVEIRA, H.P.M.; TEDESCO, A.C.; CAETANO, W.; HIOKA, N.; TESSARO, A.L. Theranostic verteporfin-loaded lipid-polymer liposome for photodynamic applications. Journal of Photochemistry and Photobiology B: Biology, 2020, 212: 112039.
• 29. BRAGA, G.; CAMPANHOLI, K.S.S.; FERREIRA, S.B.S.; DE OLIVEIRA, J.H.; VANZIN, D.; BRUSCHI, M.L.; PONTES, R.M.; MARCO, P.H.; TESSARO, A.L.; HIOKA, N.; CAETANO, W. Tautomeric and aggregational dynamics of curcumin-supersaturated Pluronic nanocarriers. ACS Applied Polymer Materials, 2020, 2: 4493-4511.
• 30. DE FREITAS, C.F.; CALORI, I.R.; DA SILVA, A.C.P.; DE CASTRO, L.V.; SATO, F.; PELLOSI, D.S.; TESSARO, A.L.; CAETANO, W.; HIOKA, N. PEG-coated vesicles from Pluronic/lipid mixtures for the carrying of photoactive erythrosine derivatives. Colloids and Surfaces B: Biointerfaces, 2019, 175: 530-544.
• 31. CALORI, I.R.; JAYME, C.C.; UENO, L.T.; MACHADO, F.B.C.; TEDESCO, A.C. Theoretical and experimental studies concerning monomer/aggregates equilibrium of zinc phthalocyanine for future photodynamic action. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2019, 214: 513-521.
• 32. DE FREITAS, C.F.; CALORI, I.R.; TESSARO, A.L.; CAETANO, W.; HIOKA, N. Rapid formation of small unilamellar vesicles (SUV) through low-frequency sonication: an innovative approach. Colloids and Surfaces B: Biointerfaces, 2019, 181: 837-844.
• 33. CALORI, I.R.; TEDESCO, A.C. Aluminum chloride phthalocyanine in MCF-7: rationally accounting for state of aggregation of photosensitizers inside cells. Dyes and Pigments, 2019, 173: 107940.
• 34. CALORI, I.R.; PELLOSI, D.S.; CAETANO, W.; TEDESCO, A.C.; HIOKA, N. Laurdan as fluorescent probe to determine the critical micelle temperature of polymers from Pluronic-coated fluid phase liposomes. Journal of Molecular Liquids, 2019, 294: 111562.
• 35. JAYME, C.C.; CALORI, I.R.; CUNHA, E.M.F.; TEDESCO, A.C. Evaluation of aluminum phthalocyanine chloride and DNA interactions for the design of an advanced drug delivery system in photodynamic therapy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2018, 201: 242-248.
• 36. CAMPANHOLI, K.S.S.; GEROLA, A.P.; VILSINSKI, B.H.; DE OLIVEIRA, E.L.; DE MORAIS, F.A.P.; RABELLO, B.R.; BRAGA, G.; SILVA, E.L.; HIOKA, N.; CAETANO, W. Development of Pluronic nanocarriers comprising pheophorbide, Zn-pheophorbide, lapachol and -lapachol combined drugs: photophysical and spectroscopic studies. Dyes and Pigments, 2018, 157: 238-250.
• 37. PEREIRA, P.C.S.; PELLOSI, D.S.; COSTA, P.F.A.; ESTEV, B.M.; HIOKA, N.; CAETANO, W. Photophysical properties and interaction studies of Rose Bengal derivatives with biomimetic systems based in micellar aqueous solutions. Journal of Molecular Liquids, 2017, 230: 674-685.
• 38. JAYME, C.C.; CALORI, I.R.; TEDESCO, A.C. Spectroscopic analysis of aluminum chloride phthalocyanine in binary water/ethanol systems for the design of a new drug delivery system for photodynamic therapy cancer treatment. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2016, 153: 178-183.
• 39. JAYME, C.C.; DE PAULA, L.B.; REZENDE, N.; FRANCHI, L.P.; TEDESCO, A.C. DNA polymeric films as a support for cell growth as a new material for regenerative medicine: compatibility and applicability. Experimental Cell Research, 2017, 360: 404-412.
• 40. CALORI, I.R.; TEDESCO, A.C. Lipid vesicles loading aluminum phthalocyanine chloride: formulation properties and disaggregation upon intracellular delivery. Journal of Photochemistry and Photobiology B: Biology, 2016, 160: 240-247.
• 41. CALORI, I.R.; PELLOSI, D.S.; VANZIN, D.; CESAR, G.B.; PEREIRA, P.C.S.; POLITI, M.J.; HIOKA, N.; CAETANO, W. Distribution of xanthene dyes in DPPC vesicles: rationally accounting for drug partitioning using a membrane model. Journal of the Brazilian Chemical Society, 2016, 27: 1938-1948.
• 42. DE FREITAS, C.F.; PELLOSI, D.S.; ESTEV, B.M.; TSUBONE, T.M.; POLITI, M.J.; CAETANO, W.; HIOKA, N. Nanostructured polymeric micelles carrying xanthene dyes for photodynamic evaluation. Photochemistry and Photobiology, 2016, 92: 790-799.
• 43. PELLOSI, D.S.; CALORI, I.R.; DE PAULA, L.B.; HIOKA, N.; QUAGLIA, F.; TEDESCO, A.C. Multifunctional theranostic Pluronic mixed micelles improve targeted photoactivity of verteporfin in cancer cells. Materials Science & Engineering C, 2016, 71: 1-9.
• 1. CALORI, I.R.; GUIMARAES, A.P.P.; TEDESCO, A. C. 3D Printed Devices as Drug Delivery Systems. Advances in Pharmaceutical Technology for Drug Delivery Systems (PTDDS), Edition 1st Edition, First Published 2025, Imprint Apple Academic Press, Pages 47.

• 1. 2025 - 13th World Congress on Alternatives and Animal Use in the Life Sciences. Participant.
• 1. 2025 - Microphysiological Systems (MPS) World Summit 2025. Participant.
• 1. 2022 - 1st Symposium on Nanotechnology Applied to Medicine and Agribusiness. Participant.
• 2. 2022 - 66th Annual Meeting of the Biophysical Society, San Francisco, United State. self-aggregation of photosensitizers in breast and human brain cancer cells.
• 3. 2021 - ICONNANO - Second International Conference of Nanoscience and Nanobiotechnology, Braslia, Brazil.
• 4. 2018 - XXVII EAIC Annual Scientific Initiation Meeting, Londrina, Brazil - pluronic P123 influence on the morphology of polycaprolactone films.
• 5. 2015 - XII Latin American Meeting of Photochemistry and Photobiology, Maresias, Brazil. development and characterization of liposome containing zinc phthalocyanine and cisplatin to application as a drug delivery system in PDT.
• 6. 2015 - I International Symposium on Drug Delivery Systems, Maring, Brazil. fluorescence-lifetime imaging microscopy of aluminum chloride phthalocyanine into oral squamous cell carcinoma: a drug delivery study by lipid vesicles.