Jamie Liu
West Covina,CA
Summary
Research Accomplishments: Researched role of Dopamine Receptor’s pathway in relation to Parkinson’s disease. Mapped signaling network to determine how cells will respond to particular combinations of signals. Dedicated scientist with exceptional organizational, troubleshooting and problem-solving skills. Detail-oriented Laboratory Manager highly effective at keeping laboratories running smoothly and efficiently. Knowledgeable about managing supplies, equipment and facilities. Pursuing new professional challenges with the opportunity to make a positive impact on laboratory operations and research success.
Overview
19
19
years of professional experience
Work History
Lab Manager
Keck Graduate Institute
Claremont, CA
09.2021 - Current
- Provide training for students performing experiments and exercises on Cedex Analyzer, Vi-cell, Agilent HPLC and Blitz
- Engage student interest and support avariety of learning styles
- Enforce laboratory safety rules at all times
- Work with other teaching staff and faculty in developing new experiments and exercises
- Provide procurement support for laboratory supplies required for courses and projects including ordering, invoicing, and purchasing documents
- Provide technical support for new laboratory equipment and software
- Manage shipping and handling of all products coming in and out of laboratories
- Maintain vendor relationships and conduct negotiation when needed
- Ensure safe and proper operation of bioprocessing and analytical equipment including troubleshooting breakdowns and performing and/or arranging preventive maintenance when needed
- Schedule technician/vendor visits and participate in technician appointments
- Tracks cost and provide inputs to budgets throughout the year
- Maintain all contracts and warranties of all Bioprocessing equipment
- Participate in preparation and execution of annual lab safety audits
- Manage shipping and handling of all products coming in and out of the laboratories
- Create, improve, and manage new and existing SOPs related to Bioprocessing Department's operations
- Maintain documents/ information by keeping daily logs and equipment records in appropriate notebooks and/or online resources
- Communicates all laboratory progress, changes, and updates to all relevant team members
- Explore opportunities to add value to Bioprocessing Department by reading technical publications to keep up with the growing trends within the industry
Research Scientist/Lab Manager
Keck Graduate Institute
Claremont, CA
03.2011 - 09.2021
- Maintained core facility for FC500 flow cytometer, FC500 MPL flow cytometer, LCQ Deca XP mass spectrometer, CEQ 8000 and CEQ 8800
- Planned experiments for proteomic analysis using 2D gel, typhoon scanner, silver staining and mass spectrometry
- Assisted with design of new research SOPs and manuscripts
- Kept accurate detailed records of experiments and help in data analysis
- Ordered laboratory supplies and support laboratory operations
- Maintained laboratory equipment and related records
- Supervised students on day-to-day lab operations and routine procedures
Research Associate
California Institute of Technology
Pasadena, CA
06.2010 - 12.2010
- Performed microinjections and neurosurgeries to determine affects of neurotoxin on C57Bl/6 mice and provided post-op care
- Planned experiments, mouse perfusion for brain collection, in situ hybridization to mRNA in neuronal tissue sections for fluorescent visualization on slides or film, and tissue collection for Immunohistochemistry (antibody probes to free-floating sections) using cryostat and vibratome
Research Associate
Proteogenix
Costa Mesa, CA
09.2009 - 01.2010
- Assisted with Product and Process Development of assays
- Worked with, troubleshoot and assist with complex PCR product development
- Performed quality controls on tests and instrumentation
- Product testing and evaluation
- Maintained good record keeping and laboratory maintenance.
Research Associate
Osmetech Molecular Diagnostics
Pasadena, CA
05.2008 - 12.2008
- Performed experiments related to PCR amplification of target sequences; including experimental design, set-up, data collection and analysis
- Performed experiment related to eSensor chip detection and measurement on nucleic acid targets
- Ran general molecular biology tasks, including DNA quantification, analysis by gel electrophoresis, capillary electrophoresis and cloning
- Prepared documents, including data summaries, reports, SOPs and procedures
- Routine laboratory tasks, such as ordering supplies, laboratory organization and clean-up, equipment maintenance.
- Validated incoming data to check information accuracy and integrity while independently locating and correcting concerns
Research Technician
California Institute of Technology
Pasadena, CA
10.2001 - 05.2008
- Built Microsoft database for storage of experimental data
- As part of The Alliance for Cellular Signaling (AfCS), work involved the use of RNAi to knock down various genes of interest in G-protein signaling pathways as a means to monitor genes’ roles in individual signaling pathways as well as to determine their roles in multiple signaling pathways (crosstalk) in response to a variety of cellular inputs
- Research involved RNAi reagent development in the mouse macrophage RAW264.7 cell line (AfCS model cell type), RNAi optimization included: siRNA transfections, shRNA construction and testing (transient transfections), and production of shRNA expressing lentiviral and retroviral cell lines
- Tested large batches of siRNAs and shRNAs (single and in tandem) targeted to various genes involved in G-protein signaling pathways
- Performed extensive cloning, vector construction using Gateway Technology from Invitrogen, retroviral and lentiviral production and purification, maintained cell lines (RAW 264.7, Phoenix cells and HEK293T), harvested and cultured primary mouse macrophages (from bone marrow), transfected and infected various cell lines, RNA and protein isolation, qRT-PCR as well as traditional PCR, Western blots, Southern blots, immunprecipitations, phagocytosis of macropages derived from bone marrow and site-directed mutagenesis
- Monitored changes in G-protein coupled receptor desensitization due to various gene knockdowns
- Maintained transgenic rodent model, included setting up breeding pairs and general management of small animal colony (PCR-based genotyping)
- Publications:
- Vailable upon request
Education
BS - Biology
California State Polytechnic University, Pomona
Pomona, CA06.1998
Skills
- Equipment Maintenance
- Staff training and development
- Research and analysis
- Lab safety
- Project Management
- Good Laboratory Practices (GLP)
- Experiment Design
- Method validation
- Database Management
- Research Results Summarization
- Laboratory Supply Tracking
- Laboratory Expense Tracking
- Staff Management
- Training and mentoring
- Team Building
Timeline
Lab Manager
Keck Graduate Institute
09.2021 - Current
Research Scientist/Lab Manager
Keck Graduate Institute
03.2011 - 09.2021
Research Associate
California Institute of Technology
06.2010 - 12.2010
Research Associate
Proteogenix
09.2009 - 01.2010
Research Associate
Osmetech Molecular Diagnostics
05.2008 - 12.2008
Research Technician
California Institute of Technology
10.2001 - 05.2008
BS - Biology
California State Polytechnic University, Pomona