Jin Chu
Newark,DE
Summary
Senior scientist with 6+ years of biotechnology industry experience in assay development, validation, and quality control. Experienced with analytical ligand binding techniques such as ELISA, MSD, Luminex, SPR, BLI, Flowcytometry, and RT-PCR. Leadership role in data review, troubleshooting, and solving challengeable bioanalytical problems to meet requirements of sponsor's timeline, scientific quality, and compliance of regulatory agencies. Authors of technical documents, including SOPs, protocols, reports, and certificates of analysis. Familiarity with regulatory requirements related to clinical biomarker testing activities (e.g., FDA, EMA, ICH).
Overview
16
16
years of professional experience
1
1
Certification
Work History
Senior Research Biologist
ANP Technologies, Inc.
09.2023 - Current
- Implemented in-vitro cytotoxicity assays for ADC drugs screening
- Developed and validated ELISA assays for ADC drugs binding characterization and PK studies
- Executed HIC-HPLC for ADC drugs analysis
- Executed drug analysis using RP-HPLC platform
- Supported commercial Covid 19 LFA production and QC testing and documentation
- Participated in Quality Assurance related activities as needed
- Maintains laboratory equipment and supplies.
Senior Staff Scientist
ACROBiosystems
02.2018 - 06.2023
- Provided technical, scientific, and project leadership for comprehensive biologics services and assay development in protein characterization, kinetics/affinity analysis, functional analysis, and IVD development
- Developed bioanalytical methods for assessment of PK and immunogenicity of bio-pharmaceutical compounds in biological matrices using ligand binding based assay including SPR, BLI, ELISA, Luminex, MSD, and Flowcytometry in the manner consistent with current regulatory expectations for bioanalysis
- Supported multi-disciplined studies, generated results, solved problems, communicate with clients and internal stakeholders, and interpreted data of the scientific nature
- Troubleshoot complex assay, identify kit/material issues (ELISA, MSD ECL, and Luminex Multiplex), and resolved with vendors
- Supervised and monitored technical staff, including work assignments, performance, and development management
- Took accountability for setting up, maintaining, operating, and performing routine and non-routine maintenance on general equipment, including problem identification and resolution.
Senior Research Scientist
TDM Pharmaceutical Research, LLC
02.2017 - 10.2017
- Led PK/PD groups in method development, validation, and sample analysis to support nonclinical and clinical studies
- Designed, developed, qualified, and implemented bioanalytical ligand binding assays (LBAs) for PK and ADA assessment for clinical studies
- Developed and implemented qRT-PCR method to quantify siRNA drug contents in biological matrix samples of clinical trials
- Responsible for data analysis and interpretation of multiple assays and provided data summaries for method development, validation, and sample testing
- Prepared timely documentation and reports for drug and biomarker bioanalysis in various species and biological matrices.
Senior Research Scientist
Lewis Katz School of Medicine at Temple University, Center for Translational Medicine
09.2011 - 01.2017
- Led multiple projects investigating potential therapeutic targets for neurodegenerative diseases
- Investigated the effect of proinflammatory 5-Lipoxygenase, a potential therapeutic target for Alzheimer's disease (AD) treatment, on regulating synaptic plasticity and memory through increasing tau phosphorylation and neurofilament formation
- Investigated the retromer dysfunction that impairs tau degradation via the endosome-lysosome system
- Designed and performed experiments to understand the mechanisms of protein misfolding and protein aggregation in neurodegenerative diseases
- Published experimental results in top-tier journals.
Postdoctoral Researcher
Lewis Katz School of Medicine at Temple University, Department of Pharmacology
05.2008 - 08.2011
- Developed and validated assay for quantification of LTB4, 8-iso-PGF2α, and β-amyloid in biological matrix
- Constructed pAAV2/1 New MCS vector carrying human 5-lipoxygenase genes
- Developed and validated methods of gene delivery mediated by AAV
- Published experimental results in top-tier journals.
Education
Ph.D. - Neurobiology
Capital Medical University (CMU)
M.S. - Pharmacolgoy
Capital Medical University (CMU)
B.S. - Pharmaceutical Chemistry
Peking University
Certification
- Clinical Trial Management Certificate of UD
- ISO/IEC 17025 Certificate
Experimental Skills & Instrumentation
- Biacore SPR
- Octet BLI (Antibody Screening & Characterization, tittering reagent characterization, antibody matched pair screening and optimization, binding kinetics, small molecules affinities)
- ELISA (Molecular Devices, Ella)
- Mesoscale Discovery (MSD)
- Luminex
- Flowcytometry
- Realtime PCR
- SDS-PAGE
- Western Blot
- HPLC
- Data analysis software (e.g., GraphPad Prism, Biacore Insight, Octet BLI Discovery, SoftMax Pro, DISCOVERY WORKBENCH, CS Express, LI-COR)
Selected Publications
Neil M Bodie, Rina Hashimoto, David Connolly, Jennifer Chu, Kazuo Takayama,corresponding author and Bruce D Uhal. Design of a chimeric ACE-2/Fc-silent fusion protein with ultrahigh affinity and neutralizing capacity for SARS-CoV-2 variants. Antibody Therapeutics. 2023 Jan 20. doi: 10.1093/abt/tbad001
Chu J, Lauretti E, Praticò D. Caspase-3-dependent cleavage of Akt modulates tau phosphorylation via GSK3β kinase: implications for Alzheimer's disease. Mol Psychiatry. 2017 Jan 31. doi: 10.1038/mp.2016.214. [Epub ahead of print]
Chu J, Wisniewski T, Praticò D. GATA1-mediated transcriptional regulation of the γ-secretase activating protein increases Aβ formation in Down syndrome. Ann Neurol. 79(1):138-43(2016). PMID: 26448035
Chu J, Li JG, Hoffman NE, Stough AM, Madesh M, Praticò D. Regulation of gamma-secretase activating protein by the 5Lipoxygenase: in vitro and in vivo evidence. Sci Rep. 5:11086 (2015). PMID: 26076991
Chu J, Li JG, Hoffman NE, Madesh M, Praticò D. Degradation of gamma secretase activating protein by the ubiquitin–proteasome pathway. Journal of Neurochemistry. 133(3): 432-439 (2015). PMID: 25533523
Timeline
Senior Research Biologist
ANP Technologies, Inc.
09.2023 - Current
Senior Staff Scientist
ACROBiosystems
02.2018 - 06.2023
Senior Research Scientist
TDM Pharmaceutical Research, LLC
02.2017 - 10.2017
Senior Research Scientist
Lewis Katz School of Medicine at Temple University, Center for Translational Medicine
09.2011 - 01.2017
Postdoctoral Researcher
Lewis Katz School of Medicine at Temple University, Department of Pharmacology
05.2008 - 08.2011
Ph.D. - Neurobiology
Capital Medical University (CMU)
M.S. - Pharmacolgoy
Capital Medical University (CMU)
B.S. - Pharmaceutical Chemistry
Peking University
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