Summary
Overview
Work History
Education
Skills
Timeline
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JONATHAN VARGAS

Damascus,MD

Summary

Associate Scientist with a B.S. in Biological Sciences, with a focus on Physiology and Neurobiology. I've specialized in downstream protein purification at a large scale. Experienced in both medical and laboratory settings. Skilled in documentation, aseptic technique, cell cloning, and E. coli cell culturing, and DNA extraction via mini-prep. Dedicated to advancing biotechnological research.

Overview

7
7
years of professional experience

Work History

Associate Scientist

AstraZeneca
Gaithersburg, MD
03.2023 - Current
  • At AstraZeneca, Development Scale-Up Center (DSC), I performed a 200L-500L scale purification process for mAbs. Starting at all steps, from buffer preparation to drug substance formulation.
  • Proficient in using PK50 purification skids, as well as Akta Pilot and Unicorn Software.
  • Analyzed purification intermediates, final products, and cell culture quality using a range of analytical techniques.
  • Interpreted experimental data, and generated detailed reports and presentations.
  • Identified opportunities for process improvement through troubleshooting, literature review, and personal initiative.
  • Designed and executed experiments, with meticulous data recording, and strong analytical skills, to drive insightful conclusions and continuous process improvements.
  • Fostered a collaborative and scientifically rigorous work environment, upholding all compliance regulations, corporate guidelines, and quality to maintain a GLS standard.

Medical Assistant

Simply Slim Medical
Bethesda, MD
03.2018 - 03.2023
  • Assessed patients by measuring vital signs and administering intramuscular injections.
  • Educated patients on medications, procedures, and physician instructions.
  • Scanned, categorized, and filed patient charts for efficient record-keeping.
  • Scheduled patient appointments to optimize clinic flow.
  • Prepared treatment rooms by cleaning surfaces and restocking necessary supplies.

Research Intern

Institute of Bioscience and Biotechnology Research
Rockville, MD
08.2019 - 05.2020
  • Created variants of the ClpS protein by truncating the ClpS N-terminus, increasing effectiveness for potential protein sequencing.
  • Reviewed the literature to remain current with new procedures, and applied learnings to related research.
  • Performed polymerase chain reaction (PCR) and learned to troubleshoot PCR reactions.
  • E. coli cell cloning via heat shock and electroporation protocols.
  • Performed E. coli cell culture using aseptic technique.
  • DNA extraction with mini-prep, and purified the DNA samples via gel purification.
  • Shared research findings through presentations.
  • Conducted experiments, and maintained detailed records of methods and outcomes.

Student Ambassador

Universities at Shady Grove
Rockville, MD
09.2018 - 05.2019
  • Led daily tours of campus for prospective students and families, pointing out buildings and detailing student life and activities.
  • Helped with registration processes and applications.
  • Maintained professionalism and efficiency while working closely with leadership.
  • Cultivated a network of media and communications with professionals to maintain strong partnerships, secured positive coverage, and facilitate quick information dissemination.

Volunteer

Upcounty Consolidation Hub
Germantown, MD
03.2020 - 03.2023
  • Educate residents, and families about available services and resources.
  • Serve as a driver and help with food and meal distributions.
  • Package shelf sustainable food.
  • Help with other activities including vaccination drive, facilitating sign ins and serving as a guide to vaccination site.

Education

Bachelor of Science - Biological Sciences: Physiology and Neurobiology

University of Maryland - College Park
College Park, MD
05.2020

Associate of Science - Biological Sciences

Montgomery College
Germantown, MD
05.2018

Skills

  • Aseptic technique
  • Running gel electrophoresis
  • Performing PCR
  • Cell staining
  • DNA extraction
  • Cell cloning/transformation
  • Bilingual (fluent in Spanish and English)
  • Responsible, fast learner, dependable, trustworthy, dedicated
  • Microsoft Office (Word, Excel, PowerPoint)

Timeline

Associate Scientist

AstraZeneca
03.2023 - Current

Volunteer

Upcounty Consolidation Hub
03.2020 - 03.2023

Research Intern

Institute of Bioscience and Biotechnology Research
08.2019 - 05.2020

Student Ambassador

Universities at Shady Grove
09.2018 - 05.2019

Medical Assistant

Simply Slim Medical
03.2018 - 03.2023

Bachelor of Science - Biological Sciences: Physiology and Neurobiology

University of Maryland - College Park

Associate of Science - Biological Sciences

Montgomery College

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