JUIFANG FAN
Elk Grove,CA
Summary
Experience and passionate Research Associate with 6 years of analyzed and hands-on experience in cell culture, cancer research, and clinical studies expertise. Skills in document handling, record keeping, and data analysis. Works with management to ensure efficient implementation of the project.
Proficient in using Excel, SigmaPlot, SPSS Statistical Software, Spectrum Studio Software, GraphPad Prism 9, CompuSyn, and Combenefit software for inductive analysis of statistics.
Overview
11
11
years of professional experience
1
1
Certification
Work History
Research Assistant I
Sutter Health
San Francisco, CA
10.2023 - Current
- Processed patient's blood samples and brain tissue for detection and analysis.
- Handled and analyzed the culture of HEK293 cell, HCC1937 cell, LOX-IMVI human melanoma cell line, NRAS mutant-A375 isogenic cell line, and suspension cell including media preparation, cell counting, and staining.
- Assisted in the publication of research papers and submitted experimental reports.
- Handled drug screen collection understanding and analysis
a. Responsible for screening MEK Inhibitor drugs for cell viability and cytotoxicity testing.
b. Performed analysis using Combenefit software and Compusyn software. - Identified and resolved data issues and experimental operation problems to drive accuracy and integrity.
- Responsible for Immunoprecipitation assays and Western Blot analysis.
- Animal experiment
Responsible for intravenous and subcutaneous injections, and measuring the tumor size of the mice.
Research Associate
California Northstate University
Elk Grove, CALIFORNIA
03.2023 - 08.2023
- Preparing weekly experimental reports.
- Responsible for cell culture, Immunoprecipitation assays and Western Blot analysis.
- Performed quantitative analysis using Image Studio 3.1 and statistical analysis of data accuracy using GraphPad Prism 9.
Research Associate I
Academia Sinica
Taipei, Taiwan
04.2016 - 03.2017
- Involved in the instrument development of Surface-Enhanced Raman Spectroscopy (SERS). Completed the sample collection and analyzed of 140 sepsis cases in a year and reached a 100% accuracy rate.
- Handled blood samples were used to determine the classification of pathogens and monitored data management.
- Operated and monitored a sample pretreatment protocol (including lysing red blood cells, sonication, and centrifugation) to incubate the purified bacterial isolates with designated antibiotics of different concentrations.
- Operated to use SERS measurements of the incubated samples with a specially designed Raman microscope, to analyze the acquired SERS spectra to determine the resistogram of the antibiotics under test and their minimum inhibitory concentrations.
- Operated Agar Plate Dilution method culture bacteria, including Gram-positive bacteria: S. aureus, S. epidermidis；Gram-negative bacteria: E.coli, K. pneumoniae.
- Performed research and/or development work under the guidance of a principal investigator, managed research projects, formulated a project plan, established a check-in process, coordinated research, and managed a schedule.
- Assisted in the publication of national research papers, and submitted experiment reports, and presentations at large seminars.
- Assisted in administrating the progress of an experiment, ensuring that it was conducted with Good Clinical Practice (GCP) and the applicable regulatory requirements and establishing regular lines of communication to manage ongoing experiment issues at the site.
- Prepared/collected and inductive integration of all documentation needed before clinical trial including Institutional Review Board (IRB) audits, and review IRB submission dossier.
Associate Research Fellow
Chang Gung Memorial Hospital Linkou Medical Center
Taoyuan, Taiwan
02.2013 - 03.2016
- Handled and arranged a questionnaire survey on basic information about maternity, apply a one-to-one face-to-face interview with the designated subject questionnaire and completed 500 questionnaires in one year.
- Oversaw and analyzed evaluation of Pulmonary Function Test results when a baby was six months old as well as used ELISA and LC-MS for multivariate unsaturated fatty acid concentrations of omega-6 and omega-3 in urine and breast milk samples.
- Responsible for the implementation of mouse animal experiments colon cancer model and processed fresh tissue, ELISA, RT-PCR, and Western Blot.
- Responsible for the culture of stem cells including media preparation and cell counting.
- Responsible for the culture of mouse splenocytes including media preparation, cell counting, and analysis of cell size distribution and number.
- Handled and analyzed culture RAW264.7 cell and CT-26 (mouse colon cancer cell) including media preparation, cell counting, staining, and cell imaging analysis.
- Operated and monitored culture RAW264.7 cells for differentiation to become osteoclast cells.
Education
Master of Science - Basic Medical Sciences
China Medical University
Taichung, Taiwan01.2013
Bachelor of Science - Food Science, Nutrition And Biotechnology
Shih Chien University
Taipei, Taiwan06.2009
Skills
- Mouse feeding and maintenance (including tube feeding, injection, and anatomical test)
- Testing of different drugs in tumor-bearing animals
- Processing fresh tissue
- Frozen sections technology
- Paraffin embedding and sectioning technology
- Immunohistochemistry (IHC) Staining
- DNA Extraction
- Reverse Transcription–Polymerase Chain Reaction (RT-PCR)
- Western blotting
- Enzyme-linked immunosorbent assay (ELISA)
- Bacterial culture: Agar Plate Dilution method
- Cell culture
- Media preparation
- Cellular differentiation
- Surface-Enhanced Raman Spectroscopy (SERS) technology
- Spectrum Studio Software
- Immunoprecipitation (IP)
- Transfection of DNA
- Drugs Screening
- Cell Viability Assays
- Cell Cytotoxicity Assays
- Animal experiment: intravenous and subcutaneous injections
Certification
- Reducing Pain and Distress in Laboratory Mice and Rats Training - 2023
- Post-Approval Monitoring (PAM) Training - 2023
- Working with Mice in Research Training - 2023
- Biomedical Research - Basic/Refresher Training - 2023
- Biomedical Sciences RCR Training - 2023
- Biosafety/Biosecurity (BSS) Training - 2023
- Social Behavioral and Education (SBE) Sciences RCR Training - 2023
Timeline
Research Assistant I
Sutter Health
10.2023 - Current
Research Associate
California Northstate University
03.2023 - 08.2023
Research Associate I
Academia Sinica
04.2016 - 03.2017
Associate Research Fellow
Chang Gung Memorial Hospital Linkou Medical Center
02.2013 - 03.2016
Master of Science - Basic Medical Sciences
China Medical University
Bachelor of Science - Food Science, Nutrition And Biotechnology
Shih Chien University