Sahar Javadi
Los Angeles,CA
Summary
Ph.D. with over 12 years' BSL-2/2+ biology lab experience, specializing in cellular and molecular biology, neuroscience, and cancer biology. Skilled in data analysis, in vivo and in vitro experiments, and assay development for drug testing. Proven track record in collaborating on diverse research projects with multiple SOPs, peer-reviewed publications, grant proposals, and awards. Demonstrated organizational and analytical skills in both academic and industrial settings.
Overview
11
11
years of professional experience
Work History
Postdoc Fellow
Cedars Sinai Medical Center
05.2023 - Current
- Develop and execute research strategies to generate 3-D neurospheres using patient-derived iPSCs for investigating disease mechanisms and drug screening in Alzheimer’s disease
- Explore the effect of varying levels of antibiotics on cell proteomics
- Generate comprehensive protocols for the proficient operation of liquid handlers crucial in supporting diverse high-throughput research endeavors.
Senior Scientist
Eurofins
10.2022 - 05.2023
- Perform plate-based, cell-based, and analytical assay development and validations (bioassay development)
- Conduct potency and analytical testing of pharmaceutical products
- Mentor, train, and coordinate laboratory activities of other team members, demonstrating innovation, creativity, and resilience in problem-solving.
Research and Teaching Assistant
University of Wisconsin-Madison
06.2015 - 09.2022
- Strategize and implement research approaches to investigate the therapeutic effect of an FDA-approved anti-cancer drug for neurological diseases
- Characterize and establish the expression pattern and function of specific RNA-binding proteins in rodent brain
- Optimize methods to isolate and establish neural progenitor cells from mouse brain at different ages
- Validate genetically modified animal models to study neurological conditions
- Supervise students on laboratory techniques such as sample preparation, immunostaining, cell counting, and animal handling.
Data Analyst
Amol, Iran
02.2013 - 05.2015
- Executed data collection, analysis, and interpretation of health, breeding, and milk production
- Reported and presented results.
Education
Ph.D. in Neuroscience -
University of Wisconsin-Madison
MSc. in Animal Breeding and Genetics -
University of Tehran
BSc. in Animal Science -
University of Guilan
Skills
- Biochemical assays: ELISA, TR-FRET, T-Cell Activation Assay, luciferase, Lumit Immunoassay, BCA protein assay, Bradford assay, Western Blot (SDS-PAGE), silver staining, Oriole Staining, IHC, ICC, antigen retrieval assays
- Cell culture: Mammalian cell culture, human iPSCs and induced neural progenitor cells, 2-D and 3-D mouse primary neural stem cells, human mesenchymal stem cells, T cells, N2A cells, HEK293 cells Daily maintenance, cryopreservation, resuscitation, passage, characterization, media preparation, proliferation, differentiation, and viability assays
- Cell isolation: Human mesenchymal stem cells, mouse neural stem cells
- Animal experiments: Mouse and rat brain dissection and sectioning, perfusion, blood sampling, mouse handling, breeding injection, euthanizing, and behavior tests
- Molecular biology: RT-qPCR, RNA isolation and validation, DNA isolation, quantification, and genotyping (agarose and digestive enzyme), protein isolation, cloning, primer designing, virus making, gene silencing, and over-expression using shRNA technology, cell transfection and transduction, cell transformation, electrophoresis, biological sample prep, maxiprep, miniprep
- Microscopy: Bright field, IF-microscopy, confocal microscopy
- Other lab skills: GMP, stereology, 2-D and 3-D cell counting and anatomical analysis, mouse handling, breeding injection, euthanizing, and behavior tests
- Bioinformatics: RNA-seq and proteomics data analysis and interpretation, data mining, population genetics, GO analysis, pathway analysis
- Laboratory automation: Liquid handler Echo650, Combo, Bio Tek Multiflo FX, single-cell sample preparation CellenOne
- Software: GraphPad Prism, ImageJ, Microsoft Word, Excel, and PowerPoint, Stereo Investigator, Nikon, R programming, Python programming, SAS, Bio Render
Websites
Publications
Sustained correction of hippocampal neurogenic and cognitive deficits after a brief treatment by Nutlin-3 in a mouse model of fragile X syndrome., BMC Medicine, 20(1), 163, Javadi, S., Li, Y., Sheng, J., Zhao, L., Fu, Y., Wang, D., Zhao, X., 2022 RGS6 mediates effects of voluntary running on adult hippocampal neurogenesis., Cell Reports, 32(5), 25, Gao, Y., Shen, M., Gonzalez, J. C., Dong, Q., Kannan, S., Hoang, J. T., Eisinger, B. E. Pandy, J., Javadi, S., 2020 Conditioned media from AICAR-treated skeletal muscle cells increases neuronal differentiation of adult neural progenitor cells., Neuropharmacology, 145, 123-130, Moon, H. Y., Javadi, S., Stremlau, M., Yoon, K. J., Becker, B., Kang, S. U., Zhao, X., 2019
Timeline
Postdoc Fellow
Cedars Sinai Medical Center
05.2023 - Current
Senior Scientist
Eurofins
10.2022 - 05.2023
Research and Teaching Assistant
University of Wisconsin-Madison
06.2015 - 09.2022
Data Analyst
02.2013 - 05.2015
Ph.D. in Neuroscience -
University of Wisconsin-Madison
MSc. in Animal Breeding and Genetics -
University of Tehran
BSc. in Animal Science -
University of Guilan
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