Summary
Overview
Work History
Education
Skills
Languages
Software
Affiliations
References
leadership experience
presentations
travel awards
reviewer for - journals
fellowships and research grants
teaching
research
publications
Timeline
Generic
Justyne Ogdahl

Justyne Ogdahl

Amherst,Massachusetts

Summary

Dedicated Life Scientist with experience in laboratory research. Expertise in data analysis and background in biochemistry and molecular and cellular biology. Proficient in conducting laboratory experiments while mentoring on research methods and data collection procedures. Hardworking and driven with organizational and analytical skills combined with unparalleled dedication to research excellence. Passionate about increasing knowledge to drive scientific growth. Ready to apply knowledge and 10 years of experience in research.

Overview

25
25
years of professional experience

Work History

PhD candidate

University of Massachusetts
09.2018 - Current

Advisor: Dr. Peter Chien

  • Performed preliminary and exploratory data analysis of the bacterial Lon protease and studies into regulated proteolysis.
  • Designed experiments, ensuring robustness and reproducibility of results.
  • Mentored undergraduate students in laboratory techniques and research methods, fostering their academic growth and professional development.
  • Authored several peer-reviewed publications showcasing original research findings and contributions to the scientific community.
  • Presented research findings at national conferences, effectively communicating complex concepts to diverse audiences.
  • Streamlined laboratory workflow by implementing time-saving protocols, contributing to improved overall productivity.
  • Collaborated with other scientists to further scientific ideas and practices

Laboratory instructor

Mt Holyoke College
09.2010 - 05.2018
  • Enhanced student understanding of complex scientific concepts through hands-on experiments and demonstrations.
  • Collaborated with fellow instructors to share resources, ideas, and strategies for improved student outcomes.
  • Mentored student researchers, fostering independent thinking and problem-solving skills.
  • Assisted students in developing strong research skills by guiding them through the scientific method and proper data analysis techniques.
  • Observed students to assess progress with laboratory work and assign grades.

Research Scientist

Pioneer Valley Life Science Institute
05.2009 - 08.2010
  • Performed research into understand how mitochondrial dysfunction leads to neurodegeneration and other age-associated diseases such as diabetes and cancer, and the molecular, biochemical, and physiological causes of mitochondrial dysfunction
  • Utilized expertise in molecular and cellular biology techniques including PCR, qPCR, RNA and DNA isolation, protein analysis, FRET, and tissue culture.Enhanced research efficiency by optimizing experimental designs and protocols.
  • Enhanced research efficiency by optimizing experimental designs and protocols.

Research Fellow

University of Massachusetts, Amherst
08.2008 - 12.2009
  • Performed research into embryonic stem cells including setting up a tissue culture facility, maintaining existing cell lines, and deriving new embryonic stem cell lines
  • Enhanced research quality by implementing rigorous methodologies and data analysis.
  • Molecular biology techniques including PCR, qPCR, RNA and DNA isolation, and genotyping
  • Managed laboratory activities and budgets
  • Mentored undergraduates, fostering growth and enhancing a collaborative environment.
  • Maintained a safe and organized laboratory environment, adhering to all safety regulations and protocols.

Research Assistant

University of Massachusetts, Amherst
09.2003 - 01.2007
  • Investigated the mechanism of how primary muscle stem cells differentiate into muscle, undergo apoptosis or become quiescent using various molecular and cellular biology techniques: Western blots, PCR, qPCR, immunocytochemistry, in situ hybridization and tissue culture to create stable and transient cell lines.
  • Advised or assisted other labs in tissue culture techniques and best practices
  • Learned new laboratory techniques and applied expertise in carrying out enhanced experiments under supervision of senior lab members.
  • Participated in regular meetings with project supervisors, providing progress updates and discussing potential challenges or areas for improvement.
  • Worked as member of integrated project team in highly collaborative work environment.

Research Technician

University of Massachusetts, Amherst
01.1999 - 01.2002
  • Enhanced research accuracy by implementing strict quality control measures and maintaining meticulous lab records.
  • Performed research into the study of muscle cell differentiation and cell death
  • Managed inventory of laboratory supplies, anticipating needs and avoiding disruptions in research activities due to shortages or delays
  • Trained new lab members on proper techniques and equipment usage, promoting a culture of continuous learning and improvement.
  • Developed strong working relationships with vendors, securing favorable pricing on essential laboratory equipment and consumables.
  • Troubleshot technical issues with laboratory equipment promptly, minimizing downtime due to malfunctions or user errors.
  • Actively participated in lab meetings, sharing knowledge and expertise to foster a collaborative research atmosphere.
  • Assisted with training junior team members in laboratory techniques and operation of equipment.
  • Participated in research projects by designing and executing experiments.


Education

PhD candidate - MCB

University of Massachusetts, Amherst
07.2024

M.S. - MCB

University of Massachusetts, Amherst
01.2005

B.S. - Biology

University of Massachusetts, Amherst
01.2001

Skills

  • Research and Analysis
  • Self-Directed and Motivated
  • Scientific Paper Experience
  • Cell culture
  • Microbial models
  • Microscopy techniques
  • Structural biology
  • Biochemistry

Languages

American Sign Language
Professional Working

Software

GraphPad, Adobe Illustrator

Affiliations

  • Skiing, swimming, surfing

References

References available upon request.

leadership experience

  • Amherst Ballet, President, Treasurer, Board Member, 2015
  • Crocker Farm Elementary School Parent Guardian Association, President, 2015

presentations

  • American Society for Biochemistry and Molecular Biology Annual Meeting, Invited speaker, Seattle WA, 2023
  • Boston Bacterial Meeting, Invited speaker, Harvard Medical School, Cambridge, MA, 2023

travel awards

American Society for Biochemistry and Molecular Biology Annual Meeting, Seattle WA, 2023

reviewer for - journals

Journal of Biological Chemistry

fellowships and research grants

  • National Institutes of Health training grant, 20220-2021, Biotechnology Training Program, University of Massachusetts, Amherst.

teaching

  • Teaching Assistant, Seaphage Laboratory (Bio 152), Spring 2024
  • Teaching Assistant, Seaphage Laboratory (Bio 152), Spring 2023
  • Instructor, Molecular and Cellular Biology Lab, Mt Holyoke College
  • Instructor, Developmental Biology Lab, Mt Holyoke College

research

The ATPase Associated with diverse cellular Activities (AAA+) family of proteases, particularly Lon, plays a crucial role in cellular proteolysis and stress responses. The regulation of Lon is essential for maintaining protein quality control in the cell. In this study, we investigated the impact of DNA binding on Lon's biochemical activity using Caulobacter crescentus as a model system. Our results reveal that while Lon can bind both double-stranded (ds)DNA and single-stranded (ss)DNA, only ssDNA binding induces changes in Lon's biochemical activity. Upon ssDNA binding, Lon's ATP hydrolysis increases, leading to enhanced degradation of protein substrates, including physiologically important targets. We demonstrate that mutations in basic residues crucial for DNA binding result in loss of ssDNA binding, with charge-specific consequences. Mutating these residues to negatively charged glutamates induces activation like ssDNA binding, while neutralizing the charge compromises Lon's activity. Further investigation indicates that ssDNA binding increases Lon's affinity for ATP, affecting both ATP hydrolysis and proteolysis. The Lon4E mutant, mimicking a DNA-bound state with negative charges, exhibits increased catalytic activity. Additionally, Lon4E is less inhibited by ADP, supporting the idea that changes in surface electrostatics activate Lon. In contrast, the Lon4A mutant, lacking negative charges, is less active, emphasizing the role of charge accumulation in Lon's function. This study provides insights into the complex regulation of Lon protease by DNA binding, highlighting the importance of surface electrostatics in modulating Lon's enzymatic activity.

publications

  • A legacy role for DNA binding of Lon protects against genotoxic stress, Rilee D. Zeinert et al., bioRxiv, 2022, 10.1101/317677
  • C-di-GMP inhibits LonA-dependent proteolysis of TfoY in Vibrio cholerae, Joshi, A. et al., PLOS Genetics, 2020, 10.1371/journal.pgen.1008897
  • Photoperiod and Testosterone Regulate Androgen Receptor Immunostaining in the Siberian Hamster Brain, Bittman, E. L., Ehrlich, D. A., Ogdahl, J. L. & Jetton, A. E., Biology of Reproduction, 2003, 10.1095/biolreprod.103.015750
  • Drosophila Morgue is an F box/ubiquitin conjugase domain protein important for grim-reaper mediated apoptosis, Wing, J. P. et al., Nature Cell Biology, 2002, 10.1038/ncb808
  • Drosophila sickle Is a Novel grim-reaper Cell Death Activator, Wing, J. P. et al., Current Biology, 2002, 10.1016/S0960-9822(02)00687-9
  • Wu, H.-m., Wong, E., Ogdahl, J. and Cheung, A.Y. (2000), A pollen tube growth-promoting arabinogalactan protein from Nicotiana alata is similar to the tobacco TTS protein. The Plant Journal, 22: 165-176.

Timeline

PhD candidate

University of Massachusetts
09.2018 - Current

Laboratory instructor

Mt Holyoke College
09.2010 - 05.2018

Research Scientist

Pioneer Valley Life Science Institute
05.2009 - 08.2010

Research Fellow

University of Massachusetts, Amherst
08.2008 - 12.2009

Research Assistant

University of Massachusetts, Amherst
09.2003 - 01.2007

Research Technician

University of Massachusetts, Amherst
01.1999 - 01.2002

PhD candidate - MCB

University of Massachusetts, Amherst

M.S. - MCB

University of Massachusetts, Amherst

B.S. - Biology

University of Massachusetts, Amherst
Justyne Ogdahl