Kandarp Nimesh Jani
Lake Hiawatha,USA
Summary
Accomplished Research Associate with a proven track record at Y-mAbs Therapeutics, mastering cell culture techniques and protein production. Skilled in DNA/RNA extraction and Western Blot, demonstrating exceptional analytical capabilities and project management. Excelled in translating complex research into actionable insights, with a strong focus on innovation and meticulous data analysis.
Overview
8
8
years of professional experience
Work History
Research Associate
Y-mAbs Therapeutics
06.2021 - Current
- Apply technical skills to complete assigned tasks within project teams, contributing to successful project outcomes
- Optimize suspension mammalian cells (ExpiCHO-S cells) for transient transfection, enhancing transfection efficiency
- Monitor and quantify protein expression levels using SDS-PAGE, Western Blot, Fluorescence imaging and FACS techniques
- Generate stable CHO cell lines to produce vaccine candidates and antibodies
- Performed aseptic handling of mammalian cells and prepared cell banks for long-term storage
- Supported laboratory operations by monitoring equipment, maintaining a clean and safe environment, and ensuring adherence to safety protocols
- Analyze and interpret experimental data and present experimental data to different teams
- Identified and resolved experimental issues, offering insights and solutions during team discussions
- Documented all laboratory activities in notebooks and electronic systems in a timely and accurate manner
Senior Research Technician
New York University Langone Health
06.2019 - 07.2020
- Performed variety of cell and molecular biology techniques including, tissue culture, DNA/RNA extraction, MINI, MIDI and MAXI Prep, PCR, RT-PCR, Genotyping
- Processing of human bloods: isolation of human PBMC's and serum
- Maintained mammalian cells in culture for tail vein injection and drug screen experiments
- Imaged (MRI) and dosed mice for SRA's (Sponsored Research Agreements with companies include Pfizer, Novartis, TargImmune, and Merck)
- Bred and genotyped several different mouse lines: KP-Pure & TLCB lines
- Expertise inducing several epigenetic mouse lines; adenocarcinoma induction to murine epigenetic models: Intranasal (Adeno-Cre Virus)
- Expertise dosing mice with a variety of treatments: IP, PO (oral gavage needle), SubQ
- Expertise utilizing isoflurane for survival surgeries and procedures including MRI, Intranasal Induction and Ear & Toe Tagging
- Monitored tumors through MRI imaging
- Performed MDOTS (Murine Derived Organotypic Tumor Samples) and PDOTS (Patient Derived Tumor Samples) for understanding the effect of various pipeline drugs in a 3D culture system using patient biopsies/resected tissue samples of various human cancers, with a concentration in human non-small cell lung adenocarcinoma and small cell lung cancer
- This included Olaparib and PRMT1 inhibitor
- Management of -80 freezers and liquid nitrogen tank samples (both human and murine samples)
Research Technician
Dana Farber Cancer Institute
03.2017 - 05.2019
- Performed Drug Screening studies using human and murine cancer cells and normal (healthy) primary cells (Main focus: inhibitors and degraders of Cyclin Dependent Kinase 7, 9, 12/13)
- Cell Viability studies (IC50 determination) and Data Analysis
- Downstream Analysis of Drug Mechanism including Western Blotting, qPCR and Cell Cycle Analysis (FACS)
- Trained with ChIP-sequencing in Tumor Cells and Tissue for Histone Marks (H3K27Ac) and relevant Transcription Factors (INSM1 and OTX2) to identify genome wide occupancy
- Trained with CRISPR/Cas9-based genetic deletion of the lineage-specific Transcription Factors (INSM1 or OTX2) in cell lines
- Oversee various experiments by communicating closely with the manager and making sure all steps are followed precisely
- Organized and analyzed experimental data and interpreted results for projects using MS Excel & Graphpad Prism
Education
Master of Science - Biomedical Nanotechnology
Northeastern University
05-2016
Bachelor of Science - Pharmacy
University of Pune
04-2013
Skills
- DNA & RNA Extraction
- CDNA synthesis
- QPCR
- PCR
- Gel Electrophoresis
- Western Blot
- ELISA
- Flow Cytometry
- Next Generation Sequencing & Library Prep
- Cell Culture Techniques
- Cell Viability Assays
Timeline
Research Associate
Y-mAbs Therapeutics
06.2021 - Current
Senior Research Technician
New York University Langone Health
06.2019 - 07.2020
Research Technician
Dana Farber Cancer Institute
03.2017 - 05.2019
Master of Science - Biomedical Nanotechnology
Northeastern University
Bachelor of Science - Pharmacy
University of Pune
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