Karina Mulaki

• Perform high-complexity molecular testing in support of diagnostic and prognostic patient care.
• Execute NGS workflows including sample preparation, library preparation, hybridization & capture, and post-run QC review.
• Conduct PCR-based assays (qPCR, ddPCR) following validated protocols and SOPs.
• Perform nucleic acid extraction (DNA/RNA) from a variety of specimen types using manual and automated methods, ensuring sample integrity and quality.
• Conduct fragment analysis assays for clinical testing, including sample preparation, instrument setup, data review, and quality assessment.
• Perform Sanger sequencing workflows, including PCR amplification, sequencing reactions, capillary electrophoresis, and result interpretation.
• Review and verify assay quality metrics to ensure accuracy, reproducibility, and compliance with validated protocols.
• Troubleshoot extraction, amplification, and sequencing issues to maintain reliable test performance and turnaround time.
• Maintain detailed documentation in the LIS and ensure timely, accurate result reporting.
• Perform routine instrument maintenance, calibration, and troubleshooting.
• Prepare and validate buffers and in-house reagents, calibrators, and controls in advance of laboratory testing and analysis orders.
• Adhere to CAP, CLIA, and institutional regulatory requirements.
• Participate in internal audits, proficiency testing, and quality improvement initiatives.
• Assist in and conduct independent research projects, contributing to advances in pathology techniques and knowledge.
• Participate in continuing education opportunities, enhancing professional growth and remaining current with industry trends.

Additional responsibilities amongst those listed below:

• Conduct comprehensive equipment QC and calibration checks, ensuring consistent performance levels across all testing instruments used within the facility.
• Collaborate with fellow technicians to troubleshoot complex cases, resulting in successful patient outcomes.
• Reduce turnaround time for test results by optimizing workflows within the lab setting.
• Train new staff members in laboratory procedures, contributing to overall team success and cohesion.
• Uphold laboratory protocols dictated by facility, governing agencies, or special project requirements.
• Attend SCACM (South Central Association for Clinical Microbiology) conferences to stay up to date with newly upcoming procedures and devices in the field, and help implement these new protocols in our lab.

• Perform routine and complex microbiological testing in support of patient diagnosis and treatment, following established clinical protocols.
• Process and culture clinical specimens (blood, urine, respiratory, wound, and body fluids) using appropriate media and incubation conditions.
• Conduct organism identification using conventional, automated, and MALDI-TOF methods.
• Perform molecular microbiology assays (PCR-based testing) for pathogen detection.
• Perform and interpret antimicrobial susceptibility testing (AST) using manual and automated systems in accordance with CLSI guidelines.
• Conduct Gram stains, acid-fast stains, and other microscopic examinations; accurately report findings.
• Monitor culture growth, recognize significant pathogens, and promptly notify appropriate clinical staff of critical results.
• Maintain strict adherence to CAP, CLIA, and other regulatory standards and laboratory safety practices.
• Perform daily quality control, instrument maintenance, and troubleshooting to ensure reliable test performance.
• Document results, QC data, and corrective actions accurately in the Laboratory Information System (LIS). Follow required procedure to report STATS and/or Critical results to clients.
• Participate in proficiency testing.
• Collaborate with microbiologists, pathologists, and healthcare teams to support timely and accurate patient care.
• Assist with training and competency assessment of new laboratory personnel.
• Support infection control and antimicrobial stewardship initiatives through accurate and timely reporting. Official microbiology liaison for the department of health, performing proper packaging and shipping of infectious materials.
• Maintain complete records of all testing performed on-site.
• Set up, maintain and verify sterility of lab equipment and tools.

• Maintained accurate inventory records to provide data for use in audits and completion of order requests
• Wrapped pallets in shrink wrap prior to loading.
• Labeled and accurately moved customer orders to meet shipment timetables and minimize errors.
• Transferred inventory to and from target destinations using forklifts and other transportation vehicles.
• Alternated goods in inventory by observing first-in/first-out approach to keep shelves organized and properly stocked.
• Prepared orders for shipment by systematically picking, packing and labeling merchandise.
• Shipped material and performed boxing, packing, labeling and preparation of any related documents.

• Preparation of patient meal trays and delivery of trays to patient rooms throughout the hospital. Kept all nursing staff informed of patient meals to ensure satisfaction of dietary requirements enlisted by their doctor.
• Operated cash register, accepting consumer payments in cash or credit card form. Counted cash drawers at the end of every shift, documenting and maintaining accuracy of money log books. Adhered to all customer service and cash handling policies and procedures.
• Ensured proper food handling procedures were followed including wrapping, labeling, dating, stocking, storing, rotating and checking temperature of food products.
• Assisted in daily stocking and inventory checks to ensure sufficient amount of fresh food was available to meet consumer needs.
• Assisted in training of all new employees, making certain they were able to proficiently carry out their job tasks, following all company guidelines.