Summary
Overview
Work History
Education
Skills
Timeline
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Kiana Anspach

Germantown,MD

Summary

Dynamic Scientific Officer with expertise in cell culture techniques, analytics, and transfection methods. Proven ability to manage multiple projects while ensuring compliance with Good Lab Practices. Recognized for innovative problem-solving and meticulous documentation, leading to successful experimental outcomes and process improvements.

Overview

8
8
years of professional experience

Work History

Scientific Officer- Protein Expression

Kemp Proteins
Frederick, MD
11.2024 - Current
  • Executes the IHC titer assay of infected cells.
  • Isolates plasmid DNA of from purified bacterial cultures
  • Conducts cell culture maintenance of both mammalian and insect cell lines, including transfection, virus infection, and the selection of stable cell lines.
  • Performs various stable and transient PEI-mediated transfections at multiple scales.
  • Manages multiple projects simultaneously while meeting deadlines efficiently.
  • Programs high-throughput bioreactors for process characterization.
  • Analyzes data from biochemical experiments to identify patterns and correlations, and presents findings to the team and clients.
  • Designs and conducts complex experiments using protocols, as well as establishing protocols, while keeping meticulous notes of unexpected changes.

Associate Scientist in Process Development

IDT-Biologika
Rockville, MD
05.2021 - 11.2024
  • Performs a variety of upstream development with mammalian and microbial cell lines (BS level 1-2), including adherent/suspension cell culture, plating/streaking bacteria, seeding in fixed bed reactor systems and fermenters, infection/transfection in adherent systems (flatware Corning T25 up to CS-10), and suspension systems (fixed bed reactor, spinner flasks, and micro carriers), and MOI studies with different viruses, MOIs, and harvest conditions.
  • Successfully adapted an adherent serum-dependent cell line to a suspension serum-free cell line (MDCK) for the client for the influenza vaccine.
  • Authors review batch records and report writing for process development and manufacturing.
  • Tech transfer process development, scale work to manufacturing by creating process parameters, reviewing records, and serving as a subject matter expert.
  • Oversaw lab equipment stock and placed orders to expand inventory.
  • Performs maintenance, calibration, and corrective action on scientific equipment.
  • Implements inventory software utilization for the team via Boxstorm.
  • Interpreted complex data sets generated by various analytical techniques, including PCR, ELISA, and SDS-PAGE assays.
  • Negotiated contracts with external vendors for laboratory supplies and services

Laboratory Technician

East Coast Laboratory
Owings Mills, MD
08.2020 - 05.2021
  • Maintain and troubleshoot lab instrumentation, including: Shimadzu LC with 4000 and 4500 Agilent MS, Indiko analyzers, Qiagen Qiacube, thermal cyclers, Luminex Magpix, and Luminex Aries.
  • Prepare samples for LC/MS examination, sequences, loads, and extraction and analysis of data.
  • Prepare and extract samples for PCR and examination, including SARS-CoV-2 testing.
  • Organizes equipment specialists' visits with demos and mechanical maintenance for all equipment.
  • Collected, labeled and stored lab samples according to laboratory specifications.
  • Processed specimens following established protocols while adhering to strict safety standards.
  • Kept lab space and equipment clean to prevent contamination with hazardous substances.
  • Kept inventory of chemicals and reagents, and sent out orders for replacement.
  • Use of a Laboratory Information Management System (LIMS) for the organization and retrieval of large amounts of data.
  • Understand and follow government mandates and protocols regarding, including but not limited to, HIPAA requirements.

Research Student Assistant

Towson University
Towson, MD
08.2017 - 12.2017
  • Maintained growth of eukaryotic cells
  • Analyzed gene expression, protein interactions, and cell signaling
  • Conducted research on mutations of E.coli
  • Performed laboratory protocol
  • Organized experimental data

Education

Certified Associate in Project Management Certificate - Project Management

Montgomery College
Germantown
03.2023

Bachelor of Science - Biology- Cell & Molecular concentration

Towson University
Towson, MD
12.2018

Skills

  • Cell culture techniques
  • Assays including: SDS-Page, FACS, IHC, TCID50
  • Vendor negotiation
  • Protein expression
  • Experimental design
  • Laboratory equipment maintenance
  • Process development
  • Technical writing
  • Inventory management
  • Root cause analysis
  • Aseptic Technique
  • Good Lab Practices (GLP)
  • Good Documentation Practices (GDP)
  • Certified Train the Trainer
  • Qiagen QiaCube Operation
  • Sartorius Ambr, Dasgip, Icellis, and Bioreactor systems
  • Nova Flex, Nucelocounter, and Vicell XR
  • Luminex Aries operation
  • Luminex MagPix instrument operation
  • OSHA Standards

Timeline

Scientific Officer- Protein Expression

Kemp Proteins
11.2024 - Current

Associate Scientist in Process Development

IDT-Biologika
05.2021 - 11.2024

Laboratory Technician

East Coast Laboratory
08.2020 - 05.2021

Research Student Assistant

Towson University
08.2017 - 12.2017

Certified Associate in Project Management Certificate - Project Management

Montgomery College

Bachelor of Science - Biology- Cell & Molecular concentration

Towson University

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Kiana Anspach