Lauren Lian

Projects:

Evaluation of CAR-Macrophage killing tumor cell in vitro _ In vitro team

Duties:

• Lentivirus titration, Murin BMP, Murine Myeloid cell isolation/expansion, Human Monocyte isolation
• Macrophage induction, Verification, Lentivirus transduction, CAR-Macrophage generation, and evaluation of CAR-Macrophage killing tumor cell in vitro, Murine T cells isolation, and verification

Projects:

Evaluation of efficacy of CAR-Macrophage to various Human or Murine tumor cells implanted mice models in vivo and ex vivo _ In vivo team

Duties:

• Human/Murine GPC3 cell line generation in vitro, or ex vivo, purification, verification, expansion, cryopreservation, validation in vitro and in vivo
• Human/Murine c-MET tumor cell line generation in vitro, or ex vivo, purification, verification, expansion, cryopreservation, validation in vitro and in vivo
• Human/Murine GPC3 or c-MET stable cell line generation and validation in vitro and in vivo
• Evaluation of multiple Human/Murine GPC3 or c-MET tumor cells growth kinetics in vivo with both xenograft and syngeneic mice models in various mice strains
• Evaluating the efficacy of CAR binders of GPC3 or c-MET to various Human or Murine tumor cells in vivo
• Duties:
• Various human and murine tumor cell line generation, selection, verification, expansion, cryopreservation, validation in vitro, in vivo and ex vivo
• Xenograft and Syngeneic mice models development/generation, observation, monitoring by measuring mice healthy conditions and body weight, subcutaneous tumor burden measurement, live bioluminescent imaging, treatment, takedown mice and various tissues harvest and procession, ex vivo tumor cell validation
• Tissue preparation for FFPE blocker, manage outsourcing services for tissue FFPE blocker preparation and IHC staining
• In vitro, in vivo and ex vivo data collection, IHC image analysis with Halo, 3DHISTEC-SlideViewer for IHC image analysis, data analysis with GraphPad Prism software, summarization and presentation
• Cryovials generation and management (banking and recording in Benchling)
• Contribute in vivo procedure protocol generation and review for GPC3 project IND application as well as research lab safety inspections
• Collaborating with other teams for taking down mice and tissue harvesting/processing
• Collaborating with in vitro teams for LNP handling, registration in Benchling, and banking
• Author and review technical documents for preparing IND submission.

Projects:

• The role of Tim3 and CEACAM1 in anti-tumor function of human affector T cells
• Extending chimeric antigen (CAR) T cell therapy to thoracic cancers
• Predictors of anti-PD1 response and relapse in front line lung cancer patients using fine needle aspirates- FNA-Merck clinical research project
• Penn-Incyte adenosine project alliance - Role of Inflammasome in Human Lung Cancer
• Kitov Pharma project - Evaluate anti-Ceacam1 antibody in CAR T cell killing to tumor cells in vitro
• Duties:
• Designing research method and generating or modifying experiment protocols based on the hypothesis and aim for each of research projects
• Executing experiments for generating the different CAR T cells in large scale and the targeted tumor cell lines by retrovirus and lentivirus transduction
• Performing CAR T cell killing assays in vitro, and ex vivo with or without the inhibition of bispecific blocking antibodies, and in vivo experiments of CAR T cell cancer immunotherapy in solid tumor NSG mice models by subcutaneously injected lung cancer cells with or without CAR T cell treatment via tail vein injection and harvesting/phenotyping tumor infiltrated T cells (TILs) from tumor nodules
• Discovering the potential mechanism by performing immunocytochemical staining for live imaging for killing, flow cytometer, immunofluorescence confocal image, scanning electron microscope (SEM) image in activated CAR T cell post interaction with tumor cells in vitro, and immunohistochemistologic assay for phenotyping TILs in the tumor nodules harvested from tumor mice models
• Handling multiple research projects under the guidance of Principal Investigator by establishing experimental plan, executing experiments, analyzing and summarizing data for research projects progress report
• Responsible for managing teamwork to performing large scale experiments and accomplishing assay data analysis and report
• Taking responsibility for collaborating tasks, coordinating with clinical research team on time and performing, producing patient oriented clinical research experiments and data report accordingly
• Engaging in data progress report and manuscript preparation as well as updating project progress report
• Managing all research lab related tasks including lab safety, ordering and managing lab supplies (e.g stocks’ in both physical location and in database), lab instruments management as well as create research material e-shipping
• Capable on teaching and training junior research staffs and biomedical students.

Projects: Discovery of LAM disease development and potential therapy on bench and clinical research

• Adhering to adherence to IRB-approved human research protocol and executing primary human LAM cell isolation from patients’ lung tissue and LAM cell culture and banking
• Designing and execute cell based assays to characterize human LAM cell phenotype as well as the mechanism on LAM cell development
• Developing and performing assays in vitro and in vivo (with potential LAM mouse model) to discover the therapies for delaying LAM disease progress
• Performing research data analysis/presentation in ATCC Annual Meeting/manuscript preparation and publication
• Engaging in lab projects progress management, collaboration, human specimen shipping/handling as well as ordering/managing lab supplies
• Playing the role as lab manager.

Projects:

The role of platelet lipids plays in hemostasis/thrombosis as well as metastasis

Duties:

• Generating/renewing animal research protocols for IACUC approval and supervising/executing lab knockout(ko) mice models generation, ko mice colonies maintaining, genotyping and phenotyping
• Establishing human/mouse primary cell lines as well as characterizing primary cells
• Providing and executing cell-based/animal based assays and bioassays (including lipids based assays)
• Responsible on producing/analyzing/presenting research data in-group meeting/regional meeting/ASH annual meeting as well as writing manuscript for publication
• Managing /executing collaborated projects and being capable training junior staffs and biomedical students.