Lenka Hromadkova

Detail-oriented team player with strong organizational skills. Ability to handle multiple projects simultaneously with a high degree of accuracy.

• CV/Resume Writing Workshop (CWRU) - 2023
• Course: Biological Mechanisms of Brain Disorders (CWRU) - 2022
• Course: Advanced Professional Development for University Teaching (CWRU) - 2022
• Processing and Analysis of Microscopic Images in Biomedicine (BIOCEV, Prague) - 2017
• Certificate of Professional Competence to Design Experiments and Experimental under Section 15d (3) of Act No 246/1992 Coll., on the Protection of Animals against Cruelty, as amended, Czech Republic (Charles University, Prague) - 2015
• School of Molecular Biotechnology - Medical Nanotechnologies: Regulated and Functional Peptides and Proteins for Tissue Engineering (ContiPro, Dolni Dobrouc, CZ) - 2014
• Course of Statistical Methods - software STATISTICA (Charles University, Prague, CZ) - 2013
• Course of Cell and Molecular Immunology (University of Pardubice, CZ) - 2013

2018: AVASTipendium for human brain from Alzheimer foundation fund: fellowship at Karolinska Institutet (Stockholm, Sweden, 6 months).

2016: Boehringer Ingelheim Fonds: fellowship at Karolinska Institutet (Stockholm, Sweden, 6 months).

2015:  EFIS Short term fellowship at Karolinska Institutet (Stockholm, Sweden, 3 months).

2014: Project DataLab fellowship at Karolinska Institutet (Stockholm, Sweden, 1 month).

2010: Fellowship for School of Molecular Biotechnology Profession, theoretical part (5 days) and Practical part (20 days) hold by CONTIPRO Holding, Dolni Dobrouc (CZ).

Honors:

2022: Trainee Oral Presentation Award – oral presentation: Modeling Alzheimer’s disease Tau protein pathogenesis in Mature Neurons: The 8th Annual Department of Pathology Clinical and Experimental Division Training Programs (11/19/2022, CWRU, Cleveland, Ohio)

2012: The Award of Dean of Faculty of Chemical Technology for the Diploma thesis “Effect of Separation Methods on the Functional Properties of Peripheral Blood Granulocytes”

Laboratory skills:

• Biochemistry techniques (immunoblotting techniques, immunoasssays, enzyme activity assays);
• Biofunctionalization of magnetic particles for biomedical applications;
• Cultures of cell lines, primary neurons and astrocytes, iPSC-derived neurons, differentiation of neuroblastoma SH-SY5Y cells;
• Tissue homogenization, cell lyses;
• Cytotoxicty and viability tests;
• Immunocytochemistry;
• Advanced fluorescence microscopic techniques, FACS;;
• Live-cell imaging;
• Mice animal research;
• BSL-2 laboratory environment

1. Hromadkova, L., Kim, C., Haldiman, T., Peng, L., Zhu, X., Cohen, M., de Silva, R., Safar, J.   G. (2023). Evolving prion-like tau conformers differentially alter postsynaptic proteins in neurons inoculated with distinct isolates of   Alzheimer’s disease tau. Cell & Bioscience. 18;13(1):174.

2. Hromadkova, L., Siddiqi, M. K., Liu, H., & Safar, J. G. (2023). Molecular Mechanisms Encoding Strains of Prions and Prion-Like Misfolded Proteins. In Prions and   Diseases (pp. 123-145). Cham: Springer International Publishing.

3. Hromadkova, L., Siddiqi, M. K., Liu, H., & Safar, J. G. (2022). Populations of Tau Conformers Drive Prion-like Strain Effects in Alzheimer’s Disease and Related   Dementias. Cells, 11(19), 2997.

4. Kim, C., Haldiman, T., Kang, S. G., Hromadkova, L., Han, Z. Z., Chen, W.,   Lissemore, F., Lerner, A., de Silva, R., Cohen, M.L., Westaway, D. & Safar, J. G. (2022). Distinct   populations of highly potent TAU seed conformers in rapidly progressing   Alzheimer’s disease. Science Translational Medicine, 14(626), eabg0253.

5. Kang, S. G., Eskandari-Sedighi, G., Hromadkova, L., Safar, J. G., &   Westaway, D. (2020). Cellular Biology of Tau Diversity and Pathogenic   Conformers. Frontiers in Neurology, 11.

6. Hromadkova, L., Bezdekova, D., Pala, J., Schedin-Weiss, S., Tjernberg, L. O., Hoschl, C., & Ovsepian, S. V. (2020). Brain-derived neurotropic factor (BDNF) promotes molecular polarization and differentiation of immature neuroblastoma cells into definitive neurons. Biochimica et Biophysica Acta (BBA)-Molecular   Cell Research, 118737.

7. Kristofikova, Z., Springer, T., Gedeonova, E., Hofmannova, A., Ricny, J., Hromadkova, L., Vyhnalek, M., Laczo, J., Nikolai, T., Hort, J. and Petrasek, T. (2020).   Interactions of 17β-Hydroxysteroid Dehydrogenase Type 10 and Cyclophilin D in   Alzheimer's Disease. Neurochemical Research, pp.1-13.

8. Hromadkova, L. and   Ovsepian, S.V. (2019). Tau-Reactive Endogenous Antibodies: Origin,   Functionality, and Implications for the Pathophysiology of Alzheimer’s   Disease. Journal of immunology research, 2019.

9. Hromadkova, L.,  Kupcik, R., Vajrychova, M., Prikryl, P., Charvatova, A., Jankovicova, B., Ripova, D., Bilkova, Z., Slovakova, M. (2018). Kinase-loaded magnetic beads for sequential in vitro phosphorylation of peptides and proteins. Analyst,   143(2), 466-474.

10. Schedin-Weiss, S., Inoue, M., Hromadkova, L., Teranishi, Y., Yamamoto, N. G.,   Wiehager, B., Bogdanovic, N., Winblad, B., Sandebring-Matton, A., Frykman,   S., Tjernberg, L. O. (2017). Monoamine oxidase B is elevated in Alzheimer disease neurons, is associated with γ-secretase and regulates neuronal amyloid β-peptide levels. Alzheimer's Research & Therapy, 9(1), 57.

11. Krestova, M., Hromadkova, L.,  Ricny, J. (2017). Purification of Natural Antibodies against Tau Protein by   Affinity Chromatography. Natural Antibodies: Methods and Protocols, 33-44. (chapter in book)

12. Hromadkova, L.,  Kupcik, R., Jankovicova, B., Rousar, T., Ripova, D., Bilkova, Z. (2016).   Difficulties associated with the structural analysis of proteins susceptible to form aggregates: The case of Tau protein as a biomarker of Alzheimer's disease. Journal of Separation Science, 39(4), 799-807.

13. Krestova, M., Hromadkova, L., Bilkova, Z., Bartos, A., Ricny, J. (2017). Characterization of isolated tau-reactive antibodies from the IVIG product,   plasma of patients with Alzheimer's disease and cognitively normal individuals. Journal of Neuroimmunology, 313, 16-24.

14. Hromadkova, L., Kolarova, M., Jankovicova, B., Bartos, A., Ricny, J., Bilkova, Z., Ripova, D. (2015). Identification and characterization of natural antibodies against tau protein in an intravenous immunoglobulin product. Journal of Neuroimmunology, 289,  121-129.

15. Jankovicova, B., Svobodova, Z., Hromadkova,   L., Kupcik, R., Ripova, D., Bilkova, Z. (2015). Benefits of Immunomagnetic Separation for Epitope Identification in Clinically Important   Protein Antigens: A Case Study Using Ovalbumin, Carbonic Anhydrase I and Tau Protein. Universal Journal of Biomedical Engineering, 3, 1-8.

16. Jankovičová, B., Hromádková, L.,  Kupčík, R., Kašparová, J., Řípová, D., Bílková, Z. (2014). Quality evaluation of monoclonal antibodies suitable for immunomagnetic purification of native   tau protein. Scientific Papers of the University of Pardubice S. A, Faculty of Chemical Technology, 20, 147-163.

17. Hromadkova, L., Zarybnicka, L., Vavrova, J., Bilkova, Z., Sinkorova, Z.  (2014). The impact of pre-analytical treatment and sorting on human neutrophil function. Veterinární Medicína, 59(11).

• Principal investigator: AARF-22-918090 (Alzheimer’s Association): 03/01/2022 – 02/28/2025