Leslie Douglas, PhD, HCLD(MD), ABB

As a High Complexity Laboratory Director, I possess extensive knowledge in compliance and oversight of laboratory services, operations, and staff. With a focus on molecular genetics and an ABB specialty of Molecular Diagnostics, I possess a wide range of abilities including report/scientific writing, department coordination, personnel management, research planning, experimental design, budget administration, client relations, project management, research presentation, data collection, policy implementation, and research management.

• HCLD(MD), ABB - American Board of Bioanalysis, 12/01/2022
• Florida Clinical Laboratory License, Dl 56712
• Hawaii Clinical Laboratory License, in process, 05/01/2024

HCLD: Provide oversight and management of all clinical laboratory and testing services functions, equipment, and vendor relations for the clinical laboratory and testing center. Ensure safe, timely, and appropriate care for all laboratory and testing center personnel/clinicians and patients.

· Documentation skills · Client relations · Clinical consultations

· Report writing abilities · Scientific writing · Laboratory management

· Department coordination · Research planning · Personnel management

· Budget administration · Project monitoring · Proposal management

· Research presentation · Data Collection · Quality Assurance/Control

· Policy Implementation · Project Planning · Presentation Skills

· Database Management · Supplies planning/maintenance

Molecular biology: gene and recombinant DNA cloning, genomic PCR, RT-PCR, qPCR, EPIC PCR, cloning via by PCR, subcloning to expression vectors, sequencing, PAGE, DNA sequencing analysis, site-directed mutagenesis, southern blot, northern blot, slot blot, dot blot, DNA isolation from bacteria and yeast, RNA extraction, poly A+ RNA selection, cDNA synthesis, oligo nucleotide primer design, DNA and cDNA library construction and screening, plasmid preparation ligation, restriction digest analysis, electroporation and transformation of recombination plasmid DNA, nucleic acid hybridization, basic cell culture, media, cell line maintenance, Lambda DNA extraction and library construction.

Bioinformatics: Advanced user of Microsoft Windows and Microsoft Office applications. Proficient in various biotechnology-related software for virtual gene cloning, DNA/RNA sequence analysis and comparisons, and uses of predictive databases: GenBank, BLAST, multiple sequence alignment, tree building using dnamL and PHYLIP, DNASTAR.

• Douglas, Leslie J, Blanche Grube, DMD. “Utilization of Whole, Fractioned, or Ground Teeth for DNA Microbial Testing: Is There a Difference?.” Townsend Letter, no. 450, January 2021, pp. 44-50.
• Grube, Blanche M, and Leslie J Douglas. “The Link Between Oral Health and Whole-Body Health: Is It Really, “All In Your Head”?.” Townsend Letter, no. 443, June 2020, pp. 21-28.
• Grube, Blanche M, and Leslie J Douglas. “How Finding A Lyme Spirochete In A Root-Canal Tooth Led To The Development Of A New Test Panel.” Townsend Letter, no. 408, July 2017, pp. 95-96.
• Douglas, Leslie. Development Of A PCR Assay For The Detection Of Lyme Borreliosos. Presented at 2016 ILADS Annual Scientific Conference. Philadelphia, PA.
• Molecular Sexing in the Mediterranean Fruit Fly, Ceratitis capitata. Insect Biochemistry and Molecular Biology 34 (2004), 159-165.
  Ribosomal ITS1 polymorphisms in Ceratitis capitata and Ceratitis rosa (Diptera: Tephritidae). Annals of the Entomological Society of America 94 (5) (2001), 726–731.
• Douglas, Leslie J. Molecular and Genetic Analysis of Ribosomal Genes in Tephritid Species. PhD Diss. University of Hawaii, Manoa. Honolulu. May, 2000. (Biomedical Sciences (Genetics)). Call no. UH Manoa Hawaiian Collection AC1 .H3 no.3923, Microfiche 39002. Also available online in pdf format at Bell & Howell (formerly UMI).
• Genetics and the Extinction of Species: DNA and the Conservation of Biodiversity. 1999. Edited by Laura F. Landweber & Andrew P. Dobson. Contributing Author, Chapter Six: Parasites and Conservation of Hawaiian Birds. Rebecca L. Cann and Leslie J. Douglas