Liaqat Ali

Molecular microbiology Research Fellow with nine years in designing, conducting and publishing results in international journals. Partnered with faculty and students to achieve excellence in preparation and performance of research related tasks, including different molecular biology techniques.

• Teaching, 3 years, COMSATS University Islamabad
• Postdoctoral Scientist, 3 years, USA
• Postdoctoral Scientist, The University of Texas at Tyler, Mechanistic Insights into VraS Mutations Linked to Bacterial Resistance
• Postdoctoral Scientist, Division of Biology, Kansas State University

• PCRBIO Research Grant 2024
• Identification of antibiotic-resistant bacteria in drinking water in Islamabad area, 2018

• BSL-2 (7 years) and BSL-3 (4 months) Lab
• Mice model
• Extraction of Plasmid and Genomic DNA from Prokaryotes and Eukaryotes
• CRISPR/Cas9
• Cloning via Lambda Red Recombineering
• Allelic Exchange Mutagenesis
• Phage Transduction
• ELISA
• Exome sequencing
• Gel electrophoresis
• Restriction enzymes analysis
• DNA sequencing analysis of mutated genes involved in hereditary disorders
• Handling of all type of PCR systems
• Statistical analysis in R
• High-throughput sequencing

• The impact of Staphylococcus aureus VraS histidine kinase mutations on antibiotic resistance, ASM Microbe, June 15-19, 2023, Houston, Texas, USA
• A benzoxazolylurea inhibits VraS and enhances antimicrobials against vancomycin intermediate-resistant Staphylococcus aureus, July 9-16, 2023, UNMC, Omaha, Nebraska, USA
• Host and aquatic environment dependent cost and benefits of the Shigella flexneri virulence plasmid and Shiga toxin production, 19th Annual Great Plains Infectious Disease Conference, November 5-6, 2021, University of Missouri, Columbia, USA
• Design and characterization of electrospun nanofiber membrane for antibacterial drug delivery, 38th Southern Biomedical Engineering Conference, August 25-28, 2022, New Orleans, USA

• Rajaa Fatima, Association of Kelch Like Family Member 10 (KLHL10) Polymorphism rs116420871(A/G) with Male Infertility
• Ayesha Faryal, Role of Crystallin Gene CRYAA in Age-Related Cataract
• Wajiha Amin, Role of EPHA2 in Causing Senile Cataract in Pakistani Population
• Ferzean Malik, Exclusion Mapping of RPE65 and LRAT in Pakistani Families with Ophthalmic Diseases
• Nizar Ali, Detection of Microbes in Drinking Water Using Heterotrophic Plate Count and Total Coliform
• Fawad Ahmad, In-silico Analysis of Canonical Splice Site Variations in USH2A Gene

• June 2022-Present, The Ben and Maytee Fisch College of Pharmacy, the University of Texas at Tyler, USA
• March 2021-2022, Division of Biology, Kansas State University, Manhattan KS, USA
• Jan 2018-2021, Department of Biosciences, COMSATS University Islamabad, Pakistan
• Sep 2014-2017, Fujian Agriculture and Forestry University, Fuzhou China
• Sep 2011-2014, Research associate at Shifa College of Medicine/Shifa International Hospital, Pakistan
• Aug 2010-2011, National Agriculture Research Centre Islamabad, Pakistan (Reproduction lab) under the project title "cryopreservation of semen"

• Molecular Mechanism of Quorum-Sensing in Enterococcus faecalis: Its Role in Virulence and Therapeutic Approaches, Liaqat Ali, Mohsan Ullah Goraya, Yasir Arafat, Muhammad Ajmal, Ji-Long Chen, Daojin Yu, Int. J. Mol. Sci., 18, 2017, 960, 10.3390/ijms18050960, 4.687, first author
• Nutrient-induced antibiotic resistance in Enterococcus faecalis in the eutrophic environment, Liaqat Ali, Yan-Qiu Wang, Jie Zhanga, Muhammad Ajmal, Zhengrun Xiao, Jiarong Wu, Ji-Long Chen, Daojin Yu, J. Glob. Antimicrob. Resist., 7, 2016, 78-83, 10.1016/j.jgar.2016.07.014, 3.022, first author
• Responses of Enterococcus faecalis resistance and cytolysin up-regulation to nutrients in constructed mesocosms, Liaqat Ali, Muhammad Mustafa, Zheng Run Xiao, Waqar Islam, Ulfat Ara, Muhammad Ajmal, Khalid Ali Khan, Hamed A Ghramh, Daojin Yu, J. King Saud Univ. Sci., 101680, 2022, 4.01, first author
• Biofilm formation plays a role in the formation of multidrug-resistant Escherichia coli toward nutrients in microcosm experiments, Xiu Ping Chen, Liaqat Ali, Li-Yun Wu, Liu Can, Chen Xiao Gang, Jing Hua Ruan, Qi Fa Huang, Song Ying Bao, Yun Ping Rao, DaoJin Yu, Frontiers in microbiology, 9, 367, 5.64, first author
• Freezability of water buffalo spermatozoa is improved with the addition of catalase in cryodiluent, Liaqat Ali, S. Murtaza Hassan Andrabi, Hussain Ahmed and S. Aftab Hussain Shah, Cryo letters, 38, 2, 145-154, 0.8, first author
• A benzoxazolylurea inhibits VraS and enhances antimicrobials against vancomycin intermediate-resistant Staphylococcus aureus, Sarkar, Aurijit, May H. Abdelaziz, Emerson P. Heckler, Liaqat Ali, Brittnee Cagle-White, Shrijan Bhattarai, Nicklaus Smith, and Robert A. Coover, bioRxiv, 2023, 2023-08, co-author
• NH125 Sensitizes Staphylococcus aureus to Cell Wall-Targeting Antibiotics through the Inhibition of the VraS Sensor Histidine Kinase, Bhattarai, Shrijan, Lane Marsh, Kelsey Knight, Liaqat Ali, Antonio Gomez, Allison Sunderhaus, and May H. Abdel Aziz, Microbiology Spectrum, 2023, e04861-22, co-author
• Role of 19 SNPs in 10 genes with type 2 diabetes in the Pakistani population, Khan, Netasha, Andrew D. Paterson, Delnaz Roshandel, Saadia Maqbool, Nosheen Fazal, Liaqat Ali, Raja Khurram et al., Gene, 848, 2023, 146899, co-author
• The SARS-CoV-2 B. 1.618 variant slightly alters the spike RBD–ACE2 binding affinity and is an antibody escaping variant: a computational structural perspective, Khan, A., Gui, J., Ahmad, W., Haq, I., Shahid, M., Khan, A.A., Shah, A., Khan, A., Ali, L., Anwar, Z. and Safdar, M., RSC Advances, 11, 48, 30132-30147, co-author
• Health and environmental effects of Heavy metals, Zaynab, Madiha, Rashid Al-Yahyai, Ayesha Ameen, Yasir Sharif, Liaqat Ali, Mahpara Fatima, Khalid Ali Khan, and Shuangfei Li, Journal of King Saud University-Science, 101653, 2021, co-author
• Evaluation of the Whole Proteome of Achromobacter xylosoxidans to Identify Vaccine Targets for mRNA and Peptides-Based Vaccine Designing Against the Emerging Respiratory and Lung Cancer-Causing Bacteria, Khan, Taimoor, Muhammad Abdullah, Tayyba Fatima Toor, Fahad N. Almajhdi, Muhammad Suleman, Arshad Iqbal, Liaqat Ali, Abbas Khan, Yasir Waheed, and Dong-Qing Wei, Frontiers in Medicine, 8, co-author
• Heterologous Expression and Characterization of Glycoside Hydrolase with its Potential Applications in Hyperthermic Environment, Mustafa, Muhammad, Liaqat Ali, Waqar Islam, Ali Noman, Chengzeng Zhou, Linsong Shen, Taoting Zhu et al., Saudi Journal of Biological Sciences, 2021, co-author
• Taurine treatment of retinal degeneration and cardiomyopathy in a consanguineous family with SLC6A6 taurine transporter deficiency, Muhammad Ansar, Emmanuelle Ranza, Madhur Shetty, Sohail A Paracha, Maleeha Azam, Ilse Kern, Justyna Iwaszkiewicz, Omer Farooq, Constantin J Pournaras, Ariane Malcles, Mateusz Kecik, Carlo Rivolta, Waqar Muzaffar, Aziz Qurban, Liaqat Ali, Yacine Aggoun, Federico A Santoni, Periklis Makrythanasis, Jawad Ahmed, Raheel Qamar, Muhammad T Sarwar, L Keith Henry, Stylianos E Antonarakis, Human Molecular Genetics, 29, 4, 618-623, https://doi.org/10.1093/hmg/ddz303, co-author
• A study of ACE, eNOS and MTHFR association with psoriasis in Pakistani population, Zehra Agha, Pakeeza Arzoo Shaiq, Shakeel Ahmed, Liaqat Ali, Maleeha Azam, Syeda Hafiza Benish Ali, Ghazala Kaukab, Raheel Qamar, Meta Gene, 15, 65-69, co-author
• Homozygosity mapping and targeted sanger sequencing reveal genetic defects underlying inherited retinal disease in families from pakistan, Maria, Maleeha, Muhammad Ajmal, Maleeha Azam, Nadia Khalida Waheed, Sorath Noorani Siddiqui, Bilal Mustafa, Humaira Ayub, Liaqat Ali, Sakeel Ahmad, et al., PloS one, 10, 3, e0119806, co-author
• Restoration of long-term monoculture degraded tea orchard by green and goat manures applications system, Yuhang Jiang, Yasir Arafat, Puleng Letuma, Liaqat Ali, Muhammad Tayyab, Muhammad Waqas, Weiwei Lin, Yanchun Li, Sheng Lin, Wenxiong Lin, Sustainability, 11, 4, 1011, co-author
• Innate immune responses against avian respiratory viruses, Mohsan Ullah Goraya, Liaqat Ali, Iqra Younis, Hosts and Viruses, 2017, 4, 78-87, co-author
• Effect of bovine serum albumin in extender on post-thaw quality and in vivo fertility of buffalo bull semen, Zia ur Rahman, Muhammad Anwar, Sayed Murtaza Hassan Andrabi, Abid Mehmood, Liaqat Ali and Hussain Ahmad, Buffalo Bulletin, 34, 4, co-author
• Glucose-induced resistance to ciprofloxacin and erythromycin in Enterococci, Xiao, Z., Yu, D., Liaqat Ali., Lin, J., Wang, Y., and Zhang, J., Enliven: Journal of Anesthesiology and Critical Care Medicine, 2, 8, co-author
• Assessment of Buffalo Bull Semen Quality Based on Sperm Motility Parameters, Motion Characteristics, and In Vitro Fertilization Rate, A. Sohail, S. M. H. Andrabi, M. Anwar, Liaqat Ali and A. Mehmood, Pak Vet J, 33, 1, 53-56, co-author

• Essentials of Staphylococcal Genetics and Metabolism Workshop, July 9-16, 2023, Center for Staphylococcal Research, University of Nebraska Medical Center, Omaha, Nebraska, USA
• Micro-Teaching: Become a Reflective Teacher, Faculty Development Academy, Islamabad, Pakistan, 2019