Manjari Mishra
California,USA
Summary
Post-Doctoral Scientist specializing in Cell Biology, Molecular Biology, Proteomics, Membrane Dynamics, and Nano Drug Delivery. Extensive 10 years expertise in developing and applying advanced techniques to investigate cellular processes, drug delivery systems, and disease mechanisms. Over a decade of experience with In-vitro/Ex-vivo and In-vivo models, membrane biophysics, and high-throughput proteomic approaches. Proven skills in project management, team leadership, and cross-functional collaborations in experimental research.
Overview
12
12
years of professional experience
1
1
Certification
Work History
Post-Doctoral Scientist
Stanford School of Medicine
11.2022 - Current
- Designed and conducted studies to elucidate the molecular mechanism of RMC-113, a broad-spectrum antiviral agent, focusing on its role in modulating autophagy pathways in SARS-CoV-2-infected cells
- Established and validated advanced cell-based assays, including FRET/NanoBRET, FRAP, ELISA, and click-chemistry-based chemoproteomics, to evaluate therapeutic efficacy and target engagement
- Optimized nano-drug delivery platforms for peptide therapeutics, enabling efficient payload delivery in vitro and in vivo
- Performed proteomic profiling using photoaffinity labeling and activity-based protein profiling to identify drug targets and validate them through genetic approaches
- Conducted viral propagation studies and generated knockout (KO) cell lines for SARS-CoV-2 and Dengue virus to investigate host-pathogen interactions
- Collaborated across disciplines to integrate medicinal chemistry, molecular biology, and pharmacology for therapeutic development
- Developed advanced imaging methods (two-photon microscopy, lattice light-sheet microscopy) to study autophagy and viral membrane interactions in real-time
- Conducted large-scale pharmacological screens to identify synergistic effects with RMC-113 on viral suppression
Research Scholar (Ph.D.)
IIT Bombay
01.2017 - 01.2022
- Characterized and elucidated the distinct biophysical and biomechanical properties of mycobacterial lipids, linking their structural features to pathogenic outcomes
- Identified and validated molecular targets of mycobacterial lipids using chemoproteomics, lipidomics, and cell-based assays
- Investigated the modulation of host signaling pathways, including phosphoinositol and actin dynamics, by mycobacterial lipids, employing advanced microscopy techniques such as confocal, super-resolution, and electron microscopy
- Engineered lipid-based nanocarriers derived from bacterial lipids for targeted drug delivery applications, demonstrating their efficacy in combination with anti-TB drugs
- Developed high-throughput methods to analyze host-pathogen interactions, integrating molecular cloning, gene editing, and advanced imaging techniques
- Pioneered the use of extracted bacterial lipids for the synthesis of liposomal formulations with immunostimulatory properties, achieving sustained drug release
- Published findings in high-impact journals, showcasing the therapeutic potential of bacterial lipid analogs in treating infectious diseases
- Conducted computational modeling and biophysical analysis of lipid-membrane interactions using small-angle X-ray scattering (SAXS) and isothermal titration calorimetry (ITC)
Research Associate
IIT Bombay
01.2016 - 01.2017
- Characterized TiO2 nanoparticles and their properties, evaluating their efficacy in removing endocrine disruptors through in vitro cell-based assays
- Designed and executed high-throughput screening protocols to measure the impact of nanoparticles on environmental contaminants
Product Specialist
Optho Remedies Pvt Ltd
01.2015 - 01.2016
- Managed scientific content creation, product development, and marketing strategies for ophthalmic solutions
- Enhanced product visibility through workshops, scientific discussions, and participation in corporate conferences, boosting sales and customer engagement
Master's Thesis
Rajendra Memorial Research Institute of Medical Sciences
01.2013 - 01.2015
- Investigated the modulation of PI3K/AKT signaling by miltefosine-resistant clinical isolates of L
- Donovani, uncovering key mechanisms of drug resistance
- Demonstrated the role of host signaling pathways in parasite survival, highlighting potential therapeutic targets to overcome resistance
- Utilized molecular biology techniques, including gene cloning, Western blotting, and flow cytometry, to elucidate host-pathogen interactions
Education
Ph.D. - Biophysics and Chemical Biology
IIT Bombay
IndiaMaster of Science - Pharmaceutical Biotechnology
NIPER
Bachelor of Science - Pharmacy
Uttar Pradesh Technical University
IndiaSkills
- Advanced Microscopy: Instrumentation: Confocal Microscopy, Super-Resolution Microscopy, Lattice sheet, Spinning Disk Confocal Microscopy, Multi-Photon Microscopy, Electron microscopy (SEM), Methods: Live-cell imaging, Immunostaining, Immunohistology, EM sample preparation
- FACS: Fluorescence-activated cell sorting
- Mass Spectrometry: Instrumentation: HPLC, HR-MS, GC-MS, Orbitrap, TLC Methods: Proteomics, Chemoproteomics, Lipidomics, ITRAQ, Targeted pull-down assays(GST/GFP/HA/Flag-tag), Magnetic azide beads
- Molecular Biology: DNA, RNA, protein isolation PCR, RT-PCR, Gene cloning, Site-directed mutagenesis, Protein overexpression, Protein purification, CRISPR-based gene editing, Transfection, Transformation, Lentiviral transduction, Western Blot, Dot Blot,
- Nano Drug Delivery: Liposomes, Giant Unilamellar Vesicles, Ligand-targeted nanoparticles, Stability evaluation, Encapsulation efficiency, Release kinetics
- Cell Culture: Mammalian cell line, Bacterial culture, Parasitic culture, Viral Propagation
- Cellular Assays: FRET, FRAP, NanoBRET, Calcium sensor assays, pH sensor assays, High-throughput drug screening (In-Vitro/Ex-Vivo/In-Vivo), ELISA, autophagy assays (GFP-RFPLC-3 sensor)
- Mouse Models: Handling mouse, Macrophage isolation, In-vivo efficacy studies
- Biophysical Techniques: Small-angle X-ray scattering (SAXS), Dynamic light scattering (DLS), Isothermal titration calorimetry (ITC), Differential scanning calorimetry (DSC), Anisotropy, Generalized Polarization imaging, Atomic Force Microscopy
- Software Skills: MetaboAnalyst, LipidSearch, BYONIC, Proteome discoverer, Compound Discoverer, Panther, Circos, MATLAB, Xcalibur, Skyline, Fiji, IMARIS, Cell profiler, MS Office
- Spectroscopy: UV-Visible, Fluorescence, Infrared Spectroscopy
- Bimolecular Characterization, Data Visualization, Data interpretation, Advanced Data Analysis
- Critical thinking,
- Innovative thinking
- Team Player
- Mentorship
- Research Ethics
Awards
- 2024, Pandemic Preparedness Hub Fellowship, Stanford University
- 2022, Best Ph.D. Thesis Award, IIT Bombay.
- 2020, Biophysical Society Membership and Travel Award for EMBO Conference.
- 2016, Junior Research Fellowship (CSIR, DST).
- 2015, Industrial internship Fellowship (BCIL, DBT, INDIA).
- 2013, National-level fellowships (GPAT/GATE, NIPER-JEE).
Publications
- PIP4K2C inhibition reverses autophagic flux impairment induced by SARS-CoV-2. bioRxiv. 2024 Apr 17:2024.04.15.589676. In Review Nature Communications, 2024
- Quantitative profiling of host proteome reveals a differential influence of structurally diverse mycobacterial lipids on host cytoskeleton machinery/responses. In Review Journal of Proteome Reserach, 2024
- Mycobacterium lipids mediated and PIP2-driven alter in host actin dynamics change the interactome with Nwasp, cofilin, and Arp2/3 complex proteins. In Review Small Molecule, 2024
- Mycobacterial lipid-derived drug-liposome conjugate eradicates endosome-localized mycobacteria., Journal of Controlled Release, 2023
- Monoethanolamine-induced glucose deprivation promotes apoptosis through metabolic rewiring in prostate cancer, Theranostics, 2021
- Remodeling of host cell plasma membrane by mycobacterial lipids., Scientific Reports, 2020
- Mycobacterial lipids impact host cell membrane mechanics., ACS Infectious Biology, 2020
- Biophysical characterization of mycobacterial membranes., Biochimica et Biophysica Acta, 2019
- Switching between blebbing and lamellipodia., Journal of Cell Science, 2021
- Design and expeditious synthesis of quinoline-pyrene based ratiometric fluorescent probes for targeting lysosomal pH; Chembiochem,2020
- Dynamic Remodeling of the Host Cell Membrane by Virulent Mycobacterial Sulfoglycolipid-1 Scientific Reports.2019
- Biophysical characterization of mycobacterial model membranes and their interaction with Rifabutin: Towards lipid-guided drug screening in tuberculosis”; Biochimica et Biophysica Acta (BBA) - Biomembranes,2019
- Assessment of Endocrine Disruption Potential of Selected Pharmaceuticals Using an In-vitro Assay. Journal of Indian Chemical Society, 2018
Certification
- BSL-3, Stanford University
- Confocal Microscopy, IIT Bombay, India
- Orbitrap Mass spectrometry, IIT Bombay, India
Timeline
Post-Doctoral Scientist
Stanford School of Medicine
11.2022 - Current
Research Scholar (Ph.D.)
IIT Bombay
01.2017 - 01.2022
Research Associate
IIT Bombay
01.2016 - 01.2017
Product Specialist
Optho Remedies Pvt Ltd
01.2015 - 01.2016
Master's Thesis
Rajendra Memorial Research Institute of Medical Sciences
01.2013 - 01.2015
Ph.D. - Biophysics and Chemical Biology
IIT Bombay
Master of Science - Pharmaceutical Biotechnology
NIPER
Bachelor of Science - Pharmacy
Uttar Pradesh Technical University
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