Maria-Rita Pastore
Davie, FL, US,FL
Summary
Research Scientist with a Ph.D. degree and more than 5 years of experience in the biochemistry, cell biology, molecular biology, and immunology research fields. Proficient mechanism-of-action of drugs, discover biomarkers for preventing or curing disease.
Overview
27
27
years of professional experience
Work History
Research Scientist
Nova Southeastern University
07.2021 - Current
- Conducted basic and applied research on understanding the mechanism of tyrosine kinase inhibitor, discovering novel drug and overcoming drug resistance for human deseases espectially cancer. The current project is 'Proteome Wide Network Analysis Reveals the EML4-ALK Inhibitor Drug Resistance in Non Small Cell Lung Cancer', which belongs to Moffitt lung cancer SPORE grant and is closed to the end, the manuscript is under the writing.
- Proposed research plan, design experiments, excuted the plan, analyzed and interpreted research findings and summarized data into reports, meetings and manuscripts.
- Developed new methods, shared and tought created tools to colleage and other collaborators: tandem affinity purifaction (TAP) liquid chromatography protocol for profilng protein complex in large scale; Biostatistics pipeline for phosphoproteomics; Real time cell analysis(xCELLigence system) for function validation of target proteins; Bioinformatics strategy for networks using various 'omics' data including genomics and proteomics; siRNA library screen techniques for proteomics results validation.
- Collaborated with colleagues for several other projects, which led to two publishcations, also one manuscript submitted, one manuscript submitting, and one manuscript under the preparing.
- Organized a proteomics research group (called pY group) including experts : Proteomicists, biologists, Physicians, Bioinformaticists, Biostatisticians, Chemists, and other staff and students.
- Menter potential Ph.D students.
Postdoctoral Fellow
Hampton University
10.2007 - 04.2011
- Conducted basic and applied research on phosphoproteomics and cancer biology.
- Well trained Liquid chromatography mass spectrometry technologies and strategies frome proteomics core and all other opportunities for instances trained in top level lab in MIT, and workshops provided by US HUPO conferences et al..
- Successfully mapped the epidermal growth factor receptor phosphorylation related to oncogenic mutations and tyrosine kinase inhibitor sensitivity, which has been published in Journal Proteome Research.
- Initiated and organized the Moffitt Proteomics Research Group.
- Established the pipeline for various proteomics analysis.
- Scheduled and trained technical staff members and potential Ph.D. students.
Visiting Scientist
University Of Utah
07.2009 - 08.2009
- Obtained systemically training in cutting-edge phosphoproteomics technique, concept and strategy from Dr. Forest White's lab.
- Can make the IMAC, preanalytical columns of liqid chromatography for phosphoproteomics analysis.
Research Associate
Davenport
11.2006 - 01.2007
- Designed and optimized assays for Influenza and HIV vaccine
Teacher
Kids Peace Mesabi Academies
09.1997 - 08.2000
- Teaching the theoretical and experimental courses of Medical biologoly and Medical cell biology.
Education
Ph.D. - Biochemistry
Xiamen University
Xiamen, China2006
Bachelor of Science - Bioengineer
Nanchang University
Nanchang, Jiangxi, China1997
Skills
- Proficient in cell biology: using human and murine cells for evaluating new biomarkers and drugs on inflammation and bone resorption/remodeling
- Proficient in molecular biology: RNA DNA extraction from cell culture and tissues Real-Time PCR and RT-qPCR, CRISP/CAS9, and siRNA KO transfection with Lipofectamine and Lonza Nucleofector 4D (electroporation)
- Proficient in Biochemical analysis: Protein extraction and quantification using BCA Protein assay kit Western Blot, SDS-PAGE, 2-D Electrophoresis; Gel Imaging
- Proficient in immunological analysis: ELISA, Immunofluorescence, Immunohistochemistry, Radioimmunoassay (RIA)
- Proficient in microscopy: Identification of blood cells, histopathological analysis of organs and tissues and identification of gonadal development, confocal microscope for NF-kB nuclear localization and inflammasome assembling, EVOS microscopy
- In vitro and in vivo test
- Familiarity with flow cytometry, Agilent Seahorse assay, HPLC
Accomplishments
- Published in several journals:Cancer Research, Journal Proteome Research, Biochem Pharmacology,Proteomics Clin Appl.,The International Journal of Biochemistry & Cell Biology, Cell biology international,Bulletin of biology et al.
- One manuscript was submitted, one manuscript is submitting, one is under writting and another manuscript is preparing.
- Developed phosphoproteomics and protein complex data analysis pipeline.
- Developed network analysis strategy and pipeline for drug discovery.
- Severed as reviewer for journals:ONCOGENE, CANCER, JOURNAL OF PROTEOME RESEARCH, HUMAN GENOMICS AND PROTEOMICS and JOURNAL OF PROTEOMICS.
- Orginazed a proteomics research team: Haura pY group.
- Awarded with 2011 Moffitt Scientific Symposium Oral Presentation Award and The 6th United State Human Proteome Organisation (US HUPO) Annual Conference Student Stipend Award.
Timeline
Research Scientist
Nova Southeastern University
07.2021 - Current
Visiting Scientist
University Of Utah
07.2009 - 08.2009
Postdoctoral Fellow
Hampton University
10.2007 - 04.2011
Research Associate
Davenport
11.2006 - 01.2007
Teacher
Kids Peace Mesabi Academies
09.1997 - 08.2000
Ph.D. - Biochemistry
Xiamen University
Bachelor of Science - Bioengineer
Nanchang University
Similar Profiles
Aleena ThomasAleena Thomas
Organic Chemistry Student Research at Nova Southeastern UniversityOrganic Chemistry Student Research at Nova Southeastern University
Reshma MangalReshma Mangal
Graduate Student Research Scientist at Nova Southeastern University College of PharmacyGraduate Student Research Scientist at Nova Southeastern University College of Pharmacy
Allison BuiAllison Bui
Student Success Mentor at Emilio Lorenzo - Nova Southeastern UniversityStudent Success Mentor at Emilio Lorenzo - Nova Southeastern University
Tamara FergusonTamara Ferguson
Financial Aid Officer at Nova Southeastern UniversityFinancial Aid Officer at Nova Southeastern University
Tami McCuenTami McCuen
Lead RN Weight Loss/ Hormonal Therapy Consultant at Vivo Health Solutions- Virtual Medical ClinicLead RN Weight Loss/ Hormonal Therapy Consultant at Vivo Health Solutions- Virtual Medical Clinic