Maria Elena Rieger
Cutler Bay,FL
Summary
A highly talented Sr. Clinical Laboratory Technologist with experience in performing complex molecular and immunologic tests. Working within the scope of Laboratory policies and procedures. Contributed to positive outcomes and cultural expectations of organizations by exhibiting professional behavior and laboratory expertise.
Overview
35
35
years of professional experience
1
1
Certification
Work History
Sr. Clinical Laboratory Technologist
UNIVERSITY OF MIAMI- MILLER SCHOOL OF MEDICINE
01.2016 - Current
- Performed complex molecular and immunologic tests within the scope of Laboratory policies and procedures
- Provided technical guidance and training to laboratory staff.
- Performed high complexity testing by preparing specimens for analysis and maintaining assay quality.
- Prepared and validated buffers and in-house reagents, calibrators, and controls in advance of laboratory testing and analysis orders.
Stem Cell Processing Medical Technologist
UNIVERSITY OF MIAMI – HOSPITAL AND CLINICS
11.2013 - 07.2016
- Performs technical processing procedures and associated quality functions related to autologous and allogeneic hematopoietic progenitor cells (HPC) and therapeutic cellular therapy products.
- Identifies and recommends improvements to standard operating procedures.
- Ensures all testing and processing performed adheres to departmental guidelines, safety/regulatory requirements, and quality control standards
Sr. Medical Technologist
UNIVERSITY OF MIAMI- MILLER SCHOOL OF MEDICINE
01.2011 - 01.2013
- Worked in the Molecular Typing department, performing DNA isolation methods and HLA typing using SSP and SSOP.
Sr. Medical Technologist - Tissue Typing Lab
UNIVERSITY OF MIAMI – MILLER SCHOOL OF MEDICINE
01.2009 - 01.2011
- Worked in the Tissue Typing laboratory, performing lymphocyte isolation, crossmatch testing, and histocompatibility testing.
- Followed laboratory protocols for specimen processing, analysis, and results reporting.
- Analyzed laboratory findings to check accuracy of results.
- Operated, calibrated, and maintained sophisticated instruments and equipment to facilitate quality laboratory and patient care operations.
Technologist
NATIONAL LABORATORY
01.2008 - 01.2009
- Worked in the Hematology, Serology, and Chemistry analysis department.
Sr. Medical Technologist
HOSPITAL NACIONAL
01.1989 - 01.2000
- Worked in the Blood Bank department, specifically in the Serology Area as a Pathologist.
- Responsibilities included transfusion, blood quality control, cross matching, and working with donors.
Education
Doctor in Biochemistry -
UNIVERSIDAD NACIONAL DE ASUNCION
01.1988
Certification
- American Medical Technologist (AMT) – Sr. Medical Technologist (MT), 2008
- Florida, TN 42260, 2009-03-13
Clinical Laboratory Skills
- In-depth ability to perform HLA and virology molecular procedures; analyze and interpret test and quality control results.
- Analyze test results and biochemical reactions; identify microorganisms; interpret tests and quality control results.
- Special ability to pay close attention to detail and written SOPs.
- Perform HLA typing using different methods based on Sequence-specific oligonucleotide probes (SSOP), sequence – specific primers (SSP) and Next generation sequencing (NGS)
- Perform PCR/STR Testing and DNA analysis for Chimerism determination in transplantation
- Perform molecular testing procedures for the identification and quantification of viral load disease agents, e.g. HIV, HCV, HBV, MPX2, West Nile, Zika Virus and SARS-CoV-2.
- Perform ABO Typing manual methods for deceased donors.
- Prepare samples (blood, body fluids, swabs, and FFPE Biopsy) and reagents for DNA, RNA according to laboratory policies.
- Prepare standard reagents for calibration testing.
- Participate in proficiency tests of unknown specimens and internal and external continuing education programs.
- Assist in validation of new testing procedures, e.g. STR test in SeqStudio.
- Provide training to the new staff on equipment and techniques.
- Responsible for the accuracy, interpretation and reporting of all test results according to laboratory policies.
- Perform routine maintenance on equipment, calibration and quality control for all reagents.
- Keep accurate records of QC, maintenance and workload data according to the laboratory policy and in compliance with CLIA, OSHA and other safety and risk-management guidelines.
- Demonstrates the ability to communicate technical and complex information effectively.
Us Evaluation
JSA – Josef SillnyAssociates, INC., 305-273-1616, Miami, Florida - USA, Equivalent of a U.S. degree of Bachelor of Science in Biochemistry from an accredited college or university in the United States., Gian M. Inchauspe, GMI:028, 2009-02-09
Equipment And Technology
- Maxwell MDx
- Roche Cobas Ampliprep
- Luminex MAGIX
- Luminex FlexMAP 3D
- Ingenius Elitech
- QuanStudio DX Real-Time PCR
- Lab Xpress
- Ion Torrent Sequencer
- Robosep
- Quantus Fluorometer
- Qubit 4 Fluorometer
- Nanodrop 2000
- Agilent Technologies 2200 – Tape Station
- ABI PRISM 3130 Genetic Analyzer
- SeqStudio Genetic Analyzer
Licenses
Florida, TN 42260, 2009-03-13
References
Larisa Riveron, UM Transplant, 305-498-1793 Gloria Barrueco, UMHC, 305-546-1670
Timeline
Sr. Clinical Laboratory Technologist
UNIVERSITY OF MIAMI- MILLER SCHOOL OF MEDICINE
01.2016 - Current
Stem Cell Processing Medical Technologist
UNIVERSITY OF MIAMI – HOSPITAL AND CLINICS
11.2013 - 07.2016
Sr. Medical Technologist
UNIVERSITY OF MIAMI- MILLER SCHOOL OF MEDICINE
01.2011 - 01.2013
Sr. Medical Technologist - Tissue Typing Lab
UNIVERSITY OF MIAMI – MILLER SCHOOL OF MEDICINE
01.2009 - 01.2011
Technologist
NATIONAL LABORATORY
01.2008 - 01.2009
Sr. Medical Technologist
HOSPITAL NACIONAL
01.1989 - 01.2000
Doctor in Biochemistry -
UNIVERSIDAD NACIONAL DE ASUNCION
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