Mark Moore
Denver,CO
Overview
33
33
years of professional experience
Work History
Professional Research Assistant
National Jewish Health CGEH Genomics Core Lab
01.2018 - 01.2025
Professional Research Assistant
Pulmonary and Critical Care Division UCHSC Genomics and Microarray Core Lab
01.2016 - 01.2018
Professional Research Assistant
University of Colorado Health Sciences Center
01.1998 - 01.2010
Professional Research Assistant
Denver Veteran’s Affairs Hospital
01.1992 - 01.1998
Education
BA - Molecular, Cellular, and Developmental Biology
University of Colorado
BA - Biochemistry
University of Colorado
Masters in Secondary Education - Science Certification
University of Phoenix
Skills
- Data collection
- Qualitative research
- Research design
Accomplishments
- Educated [Number] undergraduate students on clinical good practices and laboratory techniques.
- Designed new protocols and processes, streamlining research [Number]%.
- Collaborated with team of [Number] in the development of [Project name].
Languages
English
Timeline
Professional Research Assistant
National Jewish Health CGEH Genomics Core Lab
01.2018 - 01.2025
Professional Research Assistant
Pulmonary and Critical Care Division UCHSC Genomics and Microarray Core Lab
01.2016 - 01.2018
Professional Research Assistant
University of Colorado Health Sciences Center
01.1998 - 01.2010
Professional Research Assistant
Denver Veteran’s Affairs Hospital
01.1992 - 01.1998
BA - Biochemistry
University of Colorado
Masters in Secondary Education - Science Certification
University of Phoenix
BA - Molecular, Cellular, and Developmental Biology
University of Colorado
Publications
- Prostacyclin prevents pulmonary endothelial cell apoptosis induced by cigarette smoke.
- Nana-Sinkam SP, Lee JD, Sotto-Santiago S, Stearman RS, Keith RL, Choudhury Q, Cool C, Parr J, Moore MD, Bull TM, Voelkel NF, Geraci MW.
- Am J Respir Crit Care Med. 2007 Apr 1;175(7):676-85. Epub 2007 Jan 25. PMID:17255567[PubMed - indexed for MEDLINE]
- Human lung project: evaluating variance of gene expression in the human lung.
- Gruber MP, Coldren CD, Woolum MD, Cosgrove GP, Zeng C, Barón AE, Moore MD, Cool CD, Worthen GS, Brown KK, Geraci MW.
- Am J Respir Cell Mol Biol. 2006 Jul;35(1):65-71. Epub 2006 Feb 23. PMID:16498083[PubMed - indexed for MEDLINE]
- Prostacyclin synthase promoter regulation and familial pulmonary arterial hypertension.
- Nana-Sinkam P, Oyer RJ, Stearman RS, Sotto-Santiago S, Moore MD, Bull TM, Grady MC, Choudhury Q, Nemenoff RA, Lane K, Loyd JE, Geraci MW.
- Chest. 2005 Dec;128(6 Suppl):612S. No abstract available. PMID:16373865[PubMed - indexed for MEDLINE]
- Emphysema lung tissue gene expression profiling.
- Golpon HA, Coldren CD, Zamora MR, Cosgrove GP, Moore MD, Tuder RM, Geraci MW, Voelkel NF.
- Am J Respir Cell Mol Biol. 2004 Dec;31(6):595-600. Epub 2004 Jul 29. PMID:15284076[PubMed - indexed for MEDLINE]
- Prostacyclin in human non-small cell lung cancers.
- Nana-Sinkam P, Golpon H, Keith RL, Oyer RJ, Sotto-Santiago S, Moore MD, Franklin W, Nemenoff RA, Geraci MW.
- Chest. 2004 May;125(5 Suppl):141S. No abstract available. PMID:15136467[PubMed - indexed for MEDLINE]
- Hypoxia induces different genes in the lungs of rats compared with mice.
- Hoshikawa Y, Nana-Sinkam P, Moore MD, Sotto-Santiago S, Phang T, Keith RL, Morris KG, Kondo T, Tuder RM, Voelkel NF, Geraci MW.
- Physiol Genomics. 2003 Feb 6;12(3):209-19. Erratum in: Physiol Genomics. 2003 Mar 18;13(1):79. PMID:12464684[PubMed - indexed for MEDLINE]
- Manipulation of pulmonary prostacyclin synthase expression prevents murine lung cancer.
- Keith RL, Miller YE, Hoshikawa Y, Moore MD, Gesell TL, Gao B, Malkinson AM, Golpon HA, Nemenoff RA, Geraci MW.
- Cancer Res. 2002 Feb 1;62(3):734-40. PMID:11830527[PubMed - indexed for MEDLINE]
Experience
- A Lot. Over the course of 34 years, I have been involved in a large array of activities, including basic scientific research through working in Core labs delivering results to researchers. Our lab was one of the first labs in the country to do micro-arrays and we answered many of the questions about what makes a good micro-array (or sequencing) experiment. The last eight years I have been doing Next Generation sequencing, on a number of platforms, with a large array of library builds.
- Management duties:
- Managing a molecular biology lab of up to 12 people, including Ph.Ds., professional research assistants, and students.
- Overseeing compliance with State and Federal safety standards for biohazards, chemical hazards and lab maintenance.
- Taking excellent care of the lab; the research, the people, the equipment, and the budget. Includes ordering, billing, scheduling, and communicating.
- Planning ahead to ensure that everyone has what they need, when they need it; so that everything runs smoothly and efficiently.
- Research duties:
- Planning, troubleshooting, documenting, and analyzing data for experiments using a large variety of molecular biology techniques.
- Preparing Next Generation Sequencing Libraries including; 10X Single Cell, 10X Visium, mRNA Seq, Total RNA Seq, Whole Genome Seq, Whole Exome Seq, ATAC Seq, CHiP Seq, various panels including PCR based and probe based libraries.
- Next Generation Sequencing on several Platforms including; MiSeq, Ion Torrent, NovaSeq, and NextSeq 2000.
- Running Microarrays. This involved isolating Total RNA from tissue and blood, reverse transcribing the message (mRNA), amplifying and labeling the cDNA for hybridization on micro-arrays, then hybridizing, washing, staining, scanning, and QC-ing the microarrays.
- Collaborating with other labs and researchers, including teaching people from other labs how to work with DNA and RNA, and prepare their own samples for sequencing experiments. Also worked with core lab clients on experimental design.
- Using RT PCR (reverse transcription from mRNA and subsequent PCR amplification of a specific message) for a variety of applications, including;library build, cloning, sizing, sequencing, verifying the presence and quantity of transcripts of specific genes, and comparing gene expression between disease and normal samples.
- Isolating DNA and RNA from a large variety of tissues and organisms for PCR, RT PCR, Southern and Northern blotting, cloning, VNTR and SNP analysis, and sequencing.
- Managing Tissue Culture lines including; changing media, splitting, and harvesting samples for RNA, DNA, and Protein. Also participated in transfection and knock out experiments on tissue culture lines.
- Using ELISA, ELA, and Western Blotting to determine the presence and quantity of specific proteins in tissue samples. Also using Immunohistochemistry to determine expression, location, and quantification of specific proteins in fixed histology (microscope slide) samples.
- Using a wide selection of lab equipment including; centrifuges, microfuges, PCR machines, Micro Array hybridization stations, Micro-Array scanners, ELISA readers, spectrophotometers, tissue culture hoods, radiation hoods, electrophoresis equipment (PAGE, SDS PAGE, Bioanalyzer, Tapestation, and agarose gel electrophoresis), and histology, staining, and immunohistochemistry equipment.
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