Miao-Der "Sophie" Chen

• Increased client satisfaction by efficiently managing case files and promptly addressing inquiries.
• Facilitated timely resolution of legal matters by preparing and organizing essential documents.
• Organized documents to manage paper and electronic filing systems of clients.
• Filed court documents and legal pleadings with court clerk on behalf of attorneys.
• Enhanced office productivity by implementing efficient document management systems for easy retrieval and storage.
• Improved communication between attorneys and clients by scheduling meetings, providing status updates, and relaying critical information promptly.

• Analytical validation and troubleshooting of the Oncomine Dx Express Test on the Genexus NGS platform towards FDA approval of cancer liquid biopsy diagnostic technology
• Cell culture maintenance of multiple cancer cell lines using sterile technique
• Extraction of cell free total nucleic acid (cfTNA) from cancer cells culture media for feasibility testing and analytical validation studies for IVD companion diagnostic technology
• DNA and RNA extractions from FFPE tissue and cfTNA extractions from liquid biopsy specimens using the Kingfisher Flex and Genexus Purification Systems
• Perform QC validation assays and troubleshooting of Genexus Integrated Sequencer reagents
• Create contrived variant positive samples for limit of detection and reproducibility studies
• Analyze data and present results to cross functional teams during lab meetings
• Maintain detailed laboratory records on Benchling (electronic notebook) software and study worksheets

• Led, designed, troubleshot and executed several projects in fast-paced environment to produce rigorous research in mechanism study of cancer and molecular biology
• Managed and organized laboratory and transgenic animal colonies for different research projects
• Leveraged interpersonal/communication skills to supervise and mentor students and research assistants
• Cultured and managed numerous mammalian primary cells and various cancer cell lines for multiple projects
• Collaborated with protein array core team to facilitate case flow and project progress
• Studied heat shock-induced lysosomal injuries and cell death independent of mitochondrial signaling pathway
• Analyzed intra cellular lysosomal trafficking via confocal microscope
• Utilized CRISPR/Cas9 techniques in various cancer and MEF cell lines
• Developed and optimized recombinant protein expression methods in E
• Coli and sf9 Drosophila cells
• Explored post-translational modification of DIAP1 and Strica in drosophila S2 cells
• Optimized protein purification methods: Ni-NTA, anion exchange and size exclusion chromatography
• Studied tumorigenic and transgenic animal models in non-cleavable caspase-9 and Bcl-2 family members
• Mouse colony maintenance/breeding/sample harvest/ general animal surgery and embryonic experiments and analysis for 15 mouse lines

Manuscripts Reviewer
• Reviewed scientific manuscripts (unpaid work) for the scientific journals PLOS ONE and The Nucleus.

• Created and organized case profiles and paperwork for individual clients
• Managed discovery update into each case and drafted paperwork and e-filed into different court offices

• Led, managed and designed various research projects in a team-oriented environment
• Studied the mechanism of Heat Shock induced cell death, TRAIL induced cell death in prostate cancer cell lines and performed animal experiments in Caspase-9 knock-in transgenic mice
• Designed, optimized and troubleshot several molecular assays
• Prepared material for reports, presentations and submissions to peer-reviewed journal publications
• Used confocal microscopy, post-image analysis tool (ImageJ), protein purification, CRISPR Cas9 knockout cell lines for various projects

• Served as teaching assistant for undergrad Pharmacology & Toxicology lab and Human Physiology courses.
• Developed strong teaching and communication skill with undergrad students
• Checked assignments, proctored tests, and provided grades according to university standards
• Documented attendance and completed assignments to maintain full class and student records
• Mentored students through office hours and one-on-one communication
• Maintained atmosphere of academic learning to facilitate development of critical thinking skills
• Served as research assistant to manage research projects to full fill research progress in lab
• Helped postdocs and graduate students collect and analyze data to advance various research projects.

• Teaching assistant for undergrad Pharmacology & Toxicology lab and Human Physiology courses.
• Developed strong teaching and communication skill with undergrad students.
• Checked assignments, proctored tests, and provided grades according to university standards.
• Documented attendance and completed assignments to maintain full class and student records.
• Mentored students through office hours and one-on-one communication.
• Maintained atmosphere of academic learning to facilitate development of critical thinking skills.
• Served as research assistant to manage research projects to full fill research progress in lab.
• Helped postdocs and graduate students collect and analyze data to advance various research projects

• Trained in non-human primate and human embryonic stem cell culture and chimeric embryo micro-injection techniques
• Maintained multiple mouse embryonic stem cell lines and non-human primate embryonic stem cell lines
• Managed several mouse colonies and isolated embryos to perform micro-injection experiments
• Assisted in meeting regulatory requirements by coordinating documentation and filings

• Established vitrificated human embryonic stem cell line
• Performed research projects in mouse embryonic stem cell differentiation
• Collected and recorded data at various field sites for later assessment and data analysis

• Developed techniques in culturing mouse embryonic cell lines and developmental embryology experiments
• Optimized technique of vitrificated embryonic cryopreservation and derive embryonic stem cell line from frozen embryos
• Studied Methylglyoxal (MG) and Retinoic Acid (RA)-induced mouse embryonic stem cell differentiation
• Collected data and took active role in lab meeting to make progress for projects.