Michael Dorle
St. Paul,MN
Summary
Spent over 3 years during my bachelor’s degree performing novel microbiology research and spent 5 years in industry refining my skills during which time I published a coauthored scientific paper in Frontiers in Microbiology in 2020 based partly on the work I performed during my bachelor’s degree.
Overview
9
9
years of professional experience
Work History
AIS Watercraft Inspector
Crow Wing County Land Services
05.2023 - Current
- Effectively inspected watercraft for ecologically harmful invasive species and aquatic vegetation
- Decontaminated watercraft that have been identified as high risk following DNR protocols and specifications while adhering to all safety protocols
- Communicated the issues of invasive species with the public
- Took samples for further analysis
DoorDash driver
Doordash
08.2022 - Current
- Completed over 500 deliveries with an average customer rating of 4.92/5.
Lab Operator O3
Beckman Coulter
12.2022 - 01.2023
- Formulated, filled, test, and packaged chemical and biological reagent products of varied complexity according to operational plans and customer demand
- Prepared documentation for analysis and testing of samples.
Laboratory Technician II
Valley Medical and Wellness
03.2022 - 07.2022
- Performed data entry, specimen processing, and analysis of clinical samples for Flu and COVID-19 using PCR
- Prepared documentation for lab reports and patient samples.
Scientific Technician
Beckman Coulter
02.2021 - 07.2021
- Performed simple to moderately complex experiments analyzed results per written procedure or protocol in relation to the development of Immunoassays
- Provided support for teams performing verification experiments: performed immunoassay instrument maintenance duties and executed experiments per written procedure with supervision by scientific staff
- Manipulated patient samples via dilution, spiking, mixing, and/or aliquoting to achieve desired analyte concentrations or volumes
- Screened patient samples with automated immunoassays to characterize various analyte concentrations.
Microbiologist III
Perrigo
09.2020 - 12.2020
- Responsibilities included performing water and chemical sampling and environmental monitoring on equipment, performing review of data collected under approved methods, calibrating pipettes, performing gram stains, maintaining media and reagents as needed for daily microbiological testing, and performing cGMP tasks such as laboratory housekeeping, auditing documentation, and maintenance of microbiology laboratory supplies.
Microbiology Lab Technician II
Steris AST
10.2019 - 05.2020
- Was responsible for preparing, executing, and analyzing the results of assigned microbiological or chemistry testing according to ISO and FDA regulations
- Performed testing using Biological Indicators (BIs), Self-Contained Biological Indicators (SCBIs), Native Product Sterility (NPS), bioburden, Environmental Monitoring, MALDI-TOF mass spectrometry, and bacteriostasis/fungistasis.
Lab Associate
Takeda Pharmaceuticals
05.2019 - 07.2019
- Was responsible for tasks associated with the processing of validation data and documentation of conductivity, bioburden, endotoxin (LAL), and TOC tests in validation systems with minimal supervision
- Provided assistance in formatting data sheets and document binders and coordinating the routing of data from Quality Control to Quality Assurance
- Coordinated equipment release with Manufacturing following cleaning events.
Associate Microbiologist (Intern)
Accuratus Lab Services
05.2018 - 08.2018
- Performed spectroscopy enumeration R&D projects for antimicrobial testing while maintaining lab equipment and organization
- Conducted AOAC Use Dilution tests for liquid disinfectants with UV-Vis spectrophotometry measurements and cell counts, including a large variety of indicator organisms to determine an acceptable level of antimicrobial activity and concentration.
Microbiology Research Assistant
Montana State University-Department of Chemistry and Biochemistry
05.2017 - 12.2017
- Continued study that was started at the Center for Biofilm Engineering while additionally collaborating with another lab, using the metabolomics techniques of NMR and Mass Spectrometry, as well as adding multicellular magnetotactic bacteria to the study
- During this portion of the study, new funding was awarded towards its continuation by the Undergraduate Scholars Program.
Microbiology Research Assistant
Montana State University- Center for Biofilm Engineering
09.2016 - 05.2017
- Played a vital role in establishing protocols for the extraction, isolation, and observation of environmental microbes as the first member of the Hatzenpichler lab and the first undergraduate to develop BONCAT(Bioorthoganol Noncanonical Amino Acid Tagging)
- Studied the physiology of thermophilic archaea and bacteria in alkaline Yellowstone hot springs, including the use of multiple different types of incubations and assays
- The study was done mainly using the novel technique of BONCAT along with FISH and Epifluorescent microscopy
- This included the use of UV-Vis Spectrophotometry daily.
Undergraduate Researcher
Montana State University- Department of Microbiology and Immunology
09.2015 - 05.2017
- Developed a selective medium for ice-nucleating bacteria Pantoea Agglomerans
- Developed a Fusarium fungus which over produced isoleucine for control of invasive water hyacinth
- Performed daily lab functions such as sterilizing equipment, washing dishes, making media, lab note taking, and organizing supplies.
Education
Bachelor of Science in Microbiology -
Montana State University
12.2018
Skills
- Laboratory experience
- Collecting and preparing chemical and microbiological specimens
- Sterilization of laboratory equipment and medical devices
- Immunoassays
- Laboratory documentation, review of SOPs, and creation of protocols
- Isolation and testing of bacterial cultures
- Microscopy
- Production of media, buffers, and chemical reagents
- UV-Vis Spectrophotometry
- PCR
- Gram Stain
- Environmental monitoring
- MALDI-TOF
- Basic chemistry techniques
- Certified in bsl-1/bsl-2 biosafety procedures, GMP, GDP, and GLP
- Excellent with all Microsoft office programs
- Experienced with ISO and FDA regulations
Peer reviewed publications
Steward KF, Eilers B, Tripet B, Fuchs A, Dorle M, Rawle R, Soriano B, Balasubramanian N, Copié V, Bothner B and Hatzenpichler R (2020) Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis. Front. Microbiol. 11:197. doi: 10.3389/fmicb.2020.001
Achievementsawards
- Montana State University Undergraduate Scholars Program funding, 2017
- MSU Chemistry and Biochemistry Undergraduate Research Symposium 2nd place prize, 2016
- Awarded scientific funding from the Keck foundation
- Montana State University Scholarship of Academic Achievement, 2013
- Eagle Scout, 2012
Timeline
AIS Watercraft Inspector
Crow Wing County Land Services
05.2023 - Current
Lab Operator O3
Beckman Coulter
12.2022 - 01.2023
DoorDash driver
Doordash
08.2022 - Current
Laboratory Technician II
Valley Medical and Wellness
03.2022 - 07.2022
Scientific Technician
Beckman Coulter
02.2021 - 07.2021
Microbiologist III
Perrigo
09.2020 - 12.2020
Microbiology Lab Technician II
Steris AST
10.2019 - 05.2020
Lab Associate
Takeda Pharmaceuticals
05.2019 - 07.2019
Associate Microbiologist (Intern)
Accuratus Lab Services
05.2018 - 08.2018
Microbiology Research Assistant
Montana State University-Department of Chemistry and Biochemistry
05.2017 - 12.2017
Microbiology Research Assistant
Montana State University- Center for Biofilm Engineering
09.2016 - 05.2017
Undergraduate Researcher
Montana State University- Department of Microbiology and Immunology
09.2015 - 05.2017
Bachelor of Science in Microbiology -
Montana State University