Michelle Vilt

I initiate the DNA extraction of patient samples and then organize all samples with their associated molecular test, respectively. Currently, the main diagnostic test methods are Next Generation Sequencing (NGS) and Known Familial Variant testing (KFV). I assist with developing thorough QC measures and ensure adherence to their standards. My main focus is wet lab preparation, execution, and analysis (and problem solving if needed) within my professional scope. I develop and organize procedures that are efficiently integrated into our mainstream workflow.

• Design and order PCR primers for the desired region(s) for KFV testing
• Knowledgeable with considerable experience with: NovaSeq 6000, Agilent 2100 BioAnalyzer, Covaris E220evolution ultrasonicator, E-gel Power Snap Electrophoresis system, and regulatory maintenance on all the instruments

I initiated DNA extraction from Peripheral blood, Bone marrow, and paraffin embedded tissue; and RNA isolation from peripheral blood or bone marrow. I planned and organized workflow for efficiency, which would then dictate which PCR test I would set up and when. I performed several different PCR tests with the main focus being fluorescent and allele specific polymerase chain reactions (PCR) assays, as well as multiplex and real time PCR.

• Biosystems 3500(Capillary electrophoresis instrument) - Loading and analyzing
• DNA fragment analysis
• QuantStudio 7 Real-Time PCR System
• Loading and analysis of pharmacogenomics assay which utilizes real-time
  analysis
• Considerable experience with Qiagen instrumentation(Qiacube, QiaSymphony, EZ1 robots, and Qiacube Connect)
• Maintenance and routine quality control on all instruments
• Mass spectrometry for RNA quantification

My workflow was centered around growing, isolating, and identifying bacteria or yeast. After the identification, I would use "Vitek 2 System" or E-tests and disk diffusion to determine drug sensitivities and, if necessary, troubleshoot contradictory results.

• Operation and maintenance of BD BACTEC FX System
• Operation of BioFire, FilmArray panels: (Blood culture ID, Viral Respiratory ID, Meningitis/Encephalitis ID)
• Rapid immunoassay tests
• Blood culture bench
• Urine culture bench
• Proficient in making and reading Gram stains
• Trained new employees and provided instruction on procedures and techniques
• Specimen processing and specimen plating
• Reading cultured plates
• Operation of Cepheid GeneXpert and the detection of MRSA/MSSA