Muhasin Koyiloth
Cincinnati,USA
Summary
Analytical and detail-oriented researcher with a strong foundation in basic biosciences and a keen interest in translational research.
Experienced with designing and executing complex research experiments, ensuring rigorous scientific standards. Utilizes advanced analytical techniques and methodologies to derive meaningful conclusions. Strong understanding of collaborative teamwork and scientific communication, driving impactful research outcomes.
Overview
13
13
years of professional experience
1
1
Certification
Work History
Postdoctoral Research Fellow
University of Cincinnati
08.2022 - Current
- Designed and executed multiple experimental projects, managing timelines and priorities to meet research objectives and contribute to publications in peer-reviewed journals.
- Cultured and maintained diverse cancerous and non-cancerous cell lines for molecular and cellular analyses, ensuring aseptic techniques, reproducibility and data integrity.
- Developed and optimized 3D cell culture models using Matrigel to investigate cell signaling dynamics, signal diffusion, and cell matrix interactions under physiologically relevant conditions.
- Performed a wide range of cell-based assays, including reporter assays, flow cytometry, ELISA, qPCR, cell viability and invitro cell diffusion assays, and protein localization studies.
- Applied advanced imaging techniques, including light, phase contrast, fluorescence, wide-field, and confocal microscopy for cellular and subcellular visualization.
- Proficient in electrophoretic and immunoassay techniques, including gel electrophoresis, native PAGE, ELISA, and western blotting.
- Executed molecular biology procedures such as DNA/RNA isolation, cloning, mutagenesis, PCR, sequencing, and quality control for construct validation.
- Effectively communicated research findings through regular team meetings, conferences, and manuscript preparation for peer reviewed publication.
- Collaborated within a multidisciplinary research team, actively contributing to problem-solving discussions and ensuring timely, high quality project completion.
- Maintained rigorous quality control of experimental reagents and constructs to ensure data reliability, reproducibility, and compliance with research standards.
- Managed experimental records and databases, ensuring data accuracy, traceability, security, and accessibility in accordance with institutional and regulatory requirements.
- Regulatory knowledge: Strong understanding of clinical trial phases, ethical standards, and the roles of key regulatory bodies, including Institutional Review Boards (IRBs) and the FDA.
Cell Biologist
ISMO Biophotonics Pvt Ltd, IITM Research Park
12.2021 - 08.2022
- Collaborated with multidisciplinary teams to support the design and development of a microfluidic bioreactor prototype for advanced cell culture applications.
- Designed, optimized, and evaluated innovative protocols for brain organoid development, enhancing reproducibility and model fidelity.
- Engaged effectively with external collaborators and clients, facilitating research partnerships and technology exchange.
- Contributed actively for the preparation of grant application and meeting with funding bodies, group meetings, project reviews, supporting strategic planning and research advancement.
PhD Research
Indian Institute of Technology Madras
01.2016 - 03.2022
- Project: Study of cholesterol – phospholipid scramblase interaction using artificial membranes and cell lines (CSIR-UGC and DST Funded)
- Team: Sathyanarayana N Gummadi (PI & Advisor, IITM), A Gopala Krishna (Co-PI), and others
- Designed experiments to test cholesterol-phospholipid scramblase interaction through CRAC (Cholesterol Recognition Amino acids Consensus) motif Cholesterol-SCRM-1 | hPLSCR1
- Identified role of CRAC motif of human phospholipid scramblase 1 (hPLSCR1) in subcellular localization and nuclear export | International Journal of Biol Macromolecules/2022
- Identified human phospholipid scramblase ortholog SCRM1 in Caenorhabditis elegans | SCRM-1
- Demonstrated efficiency in experimental design and set up, critical evaluation and validation
- Regularly reviewing significant advancement in the filed for the project upgradation
- Publish research work in journals and present in the conferences
Teaching Assistant
Indian Institute of Technology Madras
08.2016 - 12.2021
- Teaching Assistant (BTech Biological Engineering/BT2011-Microbiology & Biochemistry Laboratory) & (MTech Bioprocess Engineering/BT5111 and BT5121-Bioprocess Engineering Lab 1 & 2)
- Explained experimental principles and their real-world applications.
- Demonstrated experimental setups and delivered instructional lectures.
- Conducted regular laboratory sessions, developed assessment materials, and evaluated student performance.
Assistant Professor (Contract)
Shivaji College, University of Delhi
01.2014 - 12.2015
- Delivered engaging lectures in Animal Science, Developmental Biology, Bioinformatics and Environmental Biology, improving student understanding and performance.
- Designed and administered internal assessment; maintained detailed academic and performance records for student progress tracking.
- Supervised Molecular Biology and Biotechnology laboratory courses, emphasizing experimental design, data analysis, and research methodology.
- Coordinated and evaluated university examinations, ensuring fairness and compliance with academic standards.
- Organized and led field trips to reinforce classroom learning through real-world biological observations.
Lecturer (Contract)
Government College Kasaragod, Kannur University
08.2012 - 12.2013
- Taught undergraduate courses in Biochemistry, Molecular Biology, Developmental Biology, and Applied Zoology, incorporating current research perspectives into instruction.
- Conducted regular internal assessments and maintained comprehensive academic records to monitor student performance.
- Administered and evaluated university examinations in accordance with institutional and academic standards.
- Mentored and trained students for national competitive examinations, fostering critical thinking and research aptitude.
Education
PhD Candidate - Biological Chemistry
Indian Institute of Technology Madras
Chennai, India03.2022
Master of Science - Zoology
Aligarh Muslim University
India05.2010
Bachelor of Science - Zoology
University of Calicut
India05.2007
Skills
- Computational: Certified in Molecular docking
- Illustration: Certified in Adobe Illustrator, proficient in pymol and GraphPad Prism
- Leadership: Lab Coordination, guiding junior researchers, lab purchase management
- Language: Proficient in English (Oral and Written)
- Personal: Capable of working under limited supervision, quick learner & motivated individual
- Volunteering: Reviewer for undergraduate scholarly showcase (2023 and 2024), Poster judge for graduate student research forum, expert panelist for internsacpe
- Laboratory techniques
- Experimental work plans
- Interdisciplinary coordination
- Advanced data analysis
- Scientific writing and presentation
- Computational skills
- Innovation and creativity
- Project management
- Grant writing
- Bioinformatics analysis
- Manuscript authoring
Accomplishments
- Collaborated with team of scientist and microscopy expert in the development of in-vitro cell diffusion assay.
- Supervised number of project students and summer interns.
- Achieved project sucess by completing tasks with accuracy and efficiency.
Affiliations
American Association for Cancer Research
Certification
Molecular Docking from SIAS Research Forum
Molecular Phylogeny and Molecular Docking from Mizoram University, India
Certified on development of signaling pathway networks from Rajiv Gandhi Center for Biotechnology
National Eligibility Test/Junior Research Fellowship from UGC-CSIR
Languages
English
Full Professional
Arabic
Limited Working
Hindi
Professional Working
Interests
- Participating in cooking challenges to showcase culinary skills and creativity
- Gym Workouts
- Exploring various forms of art, such as movies, music, and performances, to nurture creativity and personal growth
- Adventure Travel, road trips and food tourism
- Community Cleanup
- Volunteer Work
Timeline
Postdoctoral Research Fellow
University of Cincinnati
08.2022 - Current
Cell Biologist
ISMO Biophotonics Pvt Ltd, IITM Research Park
12.2021 - 08.2022
Teaching Assistant
Indian Institute of Technology Madras
08.2016 - 12.2021
PhD Research
Indian Institute of Technology Madras
01.2016 - 03.2022
Assistant Professor (Contract)
Shivaji College, University of Delhi
01.2014 - 12.2015
Lecturer (Contract)
Government College Kasaragod, Kannur University
08.2012 - 12.2013
Master of Science - Zoology
Aligarh Muslim University
Bachelor of Science - Zoology
University of Calicut
PhD Candidate - Biological Chemistry
Indian Institute of Technology Madras
PUBLICATIONS
- Journals:
- KA Vestal, C Kattamuri, M Koyiloth, L Ongaro, JA Howard, AM Deaton., Activin E is a transforming growth factor β ligand that signals specifically through activin receptor-like kinase 7. Biochemical Journal 481 (7), 547-564. https://doi.org/10.1042/BCJ20230404
- Koyiloth, M., Gummadi, S.N., Interaction of Human Phospholipid Scramblase 1-Cholesterol via CRAC Motif is Essential for Functional Regulation and Subcellular Localization. Inter. Jour of Biol. Macromolecules. https://doi.org/10.1016/j.ijbiomac.2022.04.087. 06/2022
- Koyiloth, M., Gummadi, S.N., Regulation and function of membrane lipids: Insights from Caenorhabditis elegans. BBA Advances. https://doi.org/10.1016/j.bbadv.2022.100043 01/2022
- Koyiloth, M., Gummadi, S.N., Cholesterol interaction attenuates scramblase activity of SCRM-1 in the artificial membrane. BBA Biomembranes.;1863(9):183548, https://doi.org/10.1016/j.bbamem.2020.183548. 2021
- Koyiloth, M., Gummadi, S.N., Molecular cloning and biochemical characterization of the phospholipid scramblase SCRM-1 from Caenorhabditis elegans. Eur Biophys J. 2020 49(2):163-173. https://doi.org/10.1007/s00249-020-01423-2. 2020
Conferences/Seminars
- Koyiloth M, Jonathan Feds, James A Howard, Thomas B. Thompson., Rudney Award Talk on “Prodomain Cross-Compatibility of Activin A Ligand on October 7, 2025 at University of Cincinnati
- Koyiloth M, Jonathan Feds, James A Howard, Thomas B. Thompson., A novel approach to study mechanism of heterodimerization of TGFβ ligands”. In TGFβ meeting at Niagara Falls Convention Center, from July 21, 2024 - July 25, 2024
- Koyiloth M, Gummadi, S.N., “SCRM1 of Caenorhabditis elegans is a human phospholipid scramblase ortholog”. In EMBO-FEBS Lecture Course on “Lipids, Proteins and their Interactions in Organelle Biology” from 29th May, 2022, to June 4th, 2022 at Spetses, Greece.
Awards
Rudney Departmental postdoc award for the year 2025, Travel award worth $400 from TGFβ meeting 2024, IUPAB travel bursary award worth 1500€ to attend EMBO-FEBS Summer School, Research Fellowship (SRF) of University Grants Commission (UGC), Government of INDIA fellowship from Jan 2016 to Dec 2021