

Accomplished Biological Sciences Ph.D. with over 8 years of research experience spanning molecular biology, cell biology, microbiology, biochemistry, and biotechnology. Expertise in designing high-throughput screens and developing assays in collaboration with computational biologists.
Project: Elucidating the role of local gene neighborhood accessory proteins in mediating prokaryotic argonaute DNA cleavage. To identify and control the factors that mediate DNA interference by prokaryotic Argonaute proteins (pAgos) to accelerate pAgo development for biotechnology. pAgos recognize and cleave DNA in concert with accessory proteins such as helicases that melt duplex DNA to facilitate single-stranded cleavage. Many pAgos exist in operons or proximity to genes that encode unknown or untested function. Here we are investigating whether these host proteins enhance pAgo activity and elucidate the role they play in cleavage. Elucidating these factors that enable robust pAgo performance in diverse hosts would facilitate the development of innovative DNA manipulation tools.