Nandhini Raman
Menlo Park,USA
Summary
Accomplished scientist with extensive experience at 10x Genomics, specializing in multiparameter flow cytometry and assay development. Proven ability to lead cross-functional teams, optimize complex workflows, and deliver high-quality datasets. Adept at scientific collaboration and problem-solving, contributing to impactful research in immune profiling and translational studies.
Overview
13
13
years of professional experience
1
1
Certification
Work History
Senior Advisor (Part-Time, Contract)
LevitasBio
Menlo Park, USA
11.2025 - Current
- Provide scientific and workflow insights supporting flow cytometry and cell-based assay programs
- Collaborate with leadership, product, and R&D teams to evaluate assay needs and workflow requirements
- Enhanced collaboration through scientific discussions on sample preparation, assay workflows, and cytometry-based characterization
- Contribute to experimental strategy, assay evaluation, and data quality expectations
- Deliver technical presentations and workflow summaries
Scientist II — Flow Cytometry & Cell Culture Core Lead
10x Genomics
Pleasanton, USA
04.2023 - 09.2025
- Led Flow Cytometry and Cell Culture Cores supporting multiple cross-functional product development teams
- Designed and optimized multiparameter flow cytometry assays for translational research programs
- Developed fixation-compatible high-parameter spectral cytometry panels for immune and tissue profiling
- Executed advanced cell sorting workflows for single-cell and multiomic assays
- Supported cross-platform validation with imaging, single-cell, and spatial biology programs
- Created SOPs, technical documentation, and training materials
Associate Flow Cytometry Core Director
Gladstone Institutes
San Francisco, USA
01.2021 - 12.2021
- Designed and executed complex single-cell sorting workflows and high-parameter phenotyping experiments
- Led user training and mentored junior staff
- Developed and optimized SOPs for emerging cytometry platforms
- Provided real-time troubleshooting and ensured continuous uptime for shared instrumentation
- Collaborated on experimental design, assay development, and data analysis
- Contributed to peer-reviewed studies on HIV entry and immune remodeling using high-parameter flow and mass cytometry, ex vivo fusion assays, and immune profiling of tissue and clinical samples (Cell Reports, PLOS One, Contraception)
Research Assistant II
Baylor Institute for Immunology Research
Dallas, USA
06.2013 - 05.2016
- Characterized differentially expressed genes in psoriasis subsets using laser capture microdissection and immunofluorescence microscopy
- Developed and validated flow cytometry panels for immunophenotyping human clinical samples
- Managed biobanking workflows including Ficoll separation, cryopreservation, and long-term sample storage
- Performed maintenance, QC, sorting, and troubleshooting for BD Fortessa, Canto, and Aria II instruments
- Conducted histological analysis of skin and tonsil tissues, including sectioning, staining, and microscopy
Education
Master of Science (M.S.) - Biotechnology
University of Texas at Dallas
Richardson, TX05.2013
Bachelor of Technology (B.Tech) - Biotechnology
SRM University
Kancheepuram, Tamil Nadu05.2011
Skills
- Multiparameter flow cytometry development
- Flow cytometry techniques
- Experimental design
- Assay development
- Data analysis
- Scientific collaboration
- Cross-functional teamwork
- Quality control
- Troubleshooting techniques
- Presentation skills
- Problem solving
- Attention to detail
- High-parameter immune phenotyping
- Cell surface marker characterization
- Primary immune cell culture
- PBMC isolation and processing
- Complex sample handling
- Advanced cell sorting workflows
- Imaging assays
- Functional immune assays
- Ex vivo biological assays
- Independent experimental design
- Translational study execution
- Mechanism-focused study execution
- High-quality dataset generation
- Cross-platform validation
- Scientific data interpretation
- Technical reporting and documentation
- SOP development and workflows
Websites
Certification
- Specialist in Cytometry — SCYM(ASCP)
- International Specialist in Cytometry — SCYM(ASCPI)
- SCYM(ASCP)CM
Publications
- Walter D, Abousoud J, Drennon T, Kunihiro C, Martin K, Li Y, Raman N, Rodriguez V, Herschleb JL, Blood Collection Workflow for Interrogation of Translational Samples with Single Cell Technologies, Blood, 2023, 142, Suppl 1, 7182, 10.1182/blood-2023-190485
- Oliveira MF, Romero JP, Chung M, Williams SR, Gottscho AD, Gupta A, Pilipauskas SE, Mohabbat S, Raman N, Sukovich DJ, Patterson DM; Visium HD Development Team; Taylor SEB, High-definition spatial transcriptomic profiling of immune cell populations in colorectal cancer, Nature Genetics, 2025-06, 57, 6, 1512–1523
- Jaffe DB, Shahi P, Adams BA, Chrisman AM, Finnegan PM, Raman N, Royall AE, Tsai F, Vollbrecht T, Reyes DS, Hepler NL, Functional antibodies exhibit light chain coherence, Nature, 2022-10-26
- Seidman D, Cavrois M, Hilton JF, Roan NR, Averbach S, Takeda M, Chang E, Raman N, Greenblatt R, Shacklett BL, Smith-McCune K, Reproductive tract immune cells from pregnant women or those using depot medroxyprogesterone acetate show no excess susceptibility to HIV-1, Contraception, 2021, 103, 1, 44–47
- Cavrois M, Hilton JF, Roan NR, Takeda M, Seidman D, Averbach S, Chang E, Raman N, Greenblatt R, Shacklett BL, Smith-McCune K, Effects of contraceptives on susceptibility of immune cells to HIV-1 fusion, PLOS One, 2019, 14, 8, e0221181
- Cavrois M, Banerjee T, Mukherjee G, Raman N, Hussien R, Rodriguez BA, Vasquez J, Spitzer MH, Lazarus NH, Jones JJ, Ochsenbauer C, McCune JM, Butcher EC, Arvin AM, Sen N, Greene WC, Roan NR, Mass Cytometric Analysis of HIV Entry, Replication, and Remodeling in Tissue CD4+ T Cells, Cell Reports, 2017, 20, 4, 984–998
Timeline
Senior Advisor (Part-Time, Contract)
LevitasBio
11.2025 - Current
Scientist II — Flow Cytometry & Cell Culture Core Lead
10x Genomics
04.2023 - 09.2025
Associate Flow Cytometry Core Director
Gladstone Institutes
01.2021 - 12.2021
Research Assistant II
Baylor Institute for Immunology Research
06.2013 - 05.2016
Master of Science (M.S.) - Biotechnology
University of Texas at Dallas
Bachelor of Technology (B.Tech) - Biotechnology
SRM University