Summary
Overview
Work History
Education
Skills
References
Leadership Experience
Timeline
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Ndidiamaka Obi

Brighton,MA

Summary

Research scientist specializing in protein engineering, molecular cloning, and biophysical characterization. Expertise in translating scientific literature into effective research strategies. Demonstrated success in cross-functional collaboration, workflow troubleshooting, and innovative problem-solving in academic and translational research environments.

Overview

14
14
years of professional experience

Work History

Ph.D. Candidate

Boston University
Boston, MA
09.2017 - 08.2025
  • Practiced statistical modelling and hypothesis testing in R during self-directed study.
  • Applied R to clean, transform, and analyze experimental data a part of a research report.
  • Cloned and optimized expression vectors using molecular biology techniques (PCR, restriction digestion, ligation, plasmid prep).
  • Preformed transient transfections in mammalian cells (e.g., HEK293, CHO) to express and analyze protein variants.
  • Cloned and verified constructs via restriction digestion and Sanger sequencing, optimized expression in suspension cultures.
  • Engineered the pBabe-bleo vector for transient mammalian expression of FLAG-tagged human PPAR𝜸 in HEK293F cells.
  • Purified recombinant PPAR𝜸 using anti-FLAG affinity chromatography and validated purity via SDS-PAGE.
  • Characterized protein-ligand interactions using SPR and ligand binding assays; assessed secondary structure using circular dichroism (CD) spectroscopy.
  • Engineered the pET-15b. vector to express His-tagged FABP4 in E.coli BL21(DE3) cells for bacterial expression.
  • Purified recombinant FABP4 using Ni-NTA affinity chromatography and validated protein quality using SDS-PAGE.
  • Collaborated with team members to assess protein expression, solubility and activity in support of downstream functional assays.
  • Maintained detailed experimental records, contributed to protocol optimization and presented findings at group meetings.

Research Associate

Elanco
Gaithersburg, MD
10.2013 - 08.2015
  • Maintained and updated quality control macro workbooks on expressed and purified recombinant protein produced by synthesis team.
  • Familiarized business team on improvements made to protein structure and production protocol to provide higher and better-quality yield of protein to be sold as supplement for agriculture feed.
  • Presented data in liaison meetings highlighting the effects modification to protein structure and changes to protein purification protocol improved production time of high-quality protein. Used as a selling point by our business team.

Microbiology Laboratory Technician II

Eurofins
Hanover, MD
05.2011 - 10.2013
  • Labeled submitted food samples with unique barcode to allow for completed microbial tests to be tracked in ELIMS system.
  • Tested food samples for pathogens such E.coli and L. monocytogenes using RTPCR assay method.
  • Confirmed positive results using enzyme based colorimetric assay.
  • Reported all tests results of microbial levels detected in food samples via their uniquely generated barcode in ELIMS.
  • Educated new technicians on GCP and SOPs needed to complete microbial testing of food samples quickly and efficiently.

Education

Doctor of Philosophy (Ph.D.) - Molecular Biology, Cell Biology and Biochemistry

Boston University
Boston, MA
08.2025

Master of Arts - Molecular Biology, Cell Biology and Biochemistry

Boston University
Boston, MA
10-2022

Bachelor of Science (B.S) - Biochemistry

University of Maryland Baltimore Country
Baltimore, MD
06.2009

Skills

  • SPR
  • SDS-PAGE
  • Western Blotting
  • DLS
  • Protein Purification
  • Plate-Based Spectroscopy (UV/VIS)
  • AKTA
  • ELISA
  • Project management
  • Problem solving
  • Technical writing and communication
  • Independent and teamwork ability
  • Attention to detail
  • Python
  • R studio
  • Pymol
  • Alpha Fold
  • MATLAB
  • Prism

References

Provide Upon Request

Leadership Experience

Boston University Women in Chemistry, Boston University, 05/16, 05/17, Arranged classroom visits whereby PhD students conduct experiments and explain science to middle school and high school students, Organized 'Chemistry Day' at Boston University where several local high schools were invited to shadow various chemistry labs for a day and get a feel for what chemists do

Timeline

Ph.D. Candidate

Boston University
09.2017 - 08.2025

Research Associate

Elanco
10.2013 - 08.2015

Microbiology Laboratory Technician II

Eurofins
05.2011 - 10.2013

Doctor of Philosophy (Ph.D.) - Molecular Biology, Cell Biology and Biochemistry

Boston University

Master of Arts - Molecular Biology, Cell Biology and Biochemistry

Boston University

Bachelor of Science (B.S) - Biochemistry

University of Maryland Baltimore Country

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Ndidiamaka Obi