Nicolas Simonet

Highly skilled Postdoctoral Researcher with comprehensive experience in biomedical research. Distinguished for innovative approaches, analytical problem-solving skills and deep understanding of scientific methodologies. Contributed significantly to groundbreaking studies in the field. Demonstrated strong communication skills and collaborative spirit working effectively in diverse teams.

• EMBO workshop, Travel grant to attend as a selected oral presentation to the workshop of Histone variants, Planegg-Martinsried, Germany, 01/01/11, 01/01/11
• Short-term visiting research fellowship, FPI-MINECO (Spain), hosted at Rutgers University, NJ, USA, 01/01/15, 12/31/15
• Pre-doctoral FPI research fellowship, Sponsored by MINECO, Spain, 01/01/13, 12/31/16

• Simonet NG, Thackray JK, Vazquez BN, Ianni A, Espinosa-Alcantud M, Morales-Sanfrutos J, Hurtado-Bagès S, Sabidó E, Buschbeck M, Tischfield J, de la Torre C, Esteller M, Braun T, Olivella M, Serrano L, Vaquero A, SIRT7 auto-ADP-ribosylation regulates glucose starvation response through mH2A1, Sci Adv, 2020, 6, 30, 1-17
• Vazquez BN, Thackray JK, Simonet NG, Chahar S, Kane-Goldsmith N, Newkirk SJ, Lee S, Xing J, Verzi MP, An W, Vaquero A, Tischfield JA, Serrano L, SIRT7 mediates L1 elements transcriptional repression and their association with the nuclear lamina, Nucleic Acids Res, 2019, 47, 15, 7870-7885
• Rasti G, Simonet NG, Vaquero A, Niacin, Princ Nutr Nutr Fundam Individ Nutr, 2019, 287-293
• Simonet NG, Vaquero A, Raising the list of SIRT7 targets to a new level, Proteomics, 2017, 17, 13-14, 13-14
• Vazquez BN, Thackray JK, Simonet NG, Kane-Goldsmith N, Martinez- Redondo P, Nguyen T, Bunting S, Vaquero A, Tischfield JA, Serrano L, SIRT 7 promotes genome integrity and modulates non-homologous end joining DNA repair, EMBO J, 2016, 35, 14, 1488-1503
• Simonet NG, Rasti G, Vaquero A, The Histone Code and Disease: Posttranslational Modifications as Potential Prognostic Factors for Clinical Diagnosis, Elsevier Inc, 2015
• Simonet NG, Reyes M, Nardocci G, Molina A, Alvarez M, Epigenetic regulation of the ribosomal cistron seasonally modulates enrichment of H2A.Z and H2A.Zub in response to different environmental inputs in carp (Cyprinus carpio), Epigenetics and Chromatin, 2013, 6, 1, 1

Mass General Hospital, Dr. Jeannie Lee’s Lab (2021- Present)

· Epigenetic regulation of dosage compensation by SIRT7

· Regulatory role of hematopoietic Xist and mouse survival after X-rays

· Functional link between Xist RNA and ADP-ribosylation by PARP1 upon stress

· Sex-biased gene expression regulation in mouse tissues

Josep Carreras Leukaemia Research Institute, Dr. Alejandro Vaquero’s Lab (2018-2020)

· Mechanistic insights into SIRT7‑mediated X‑chromosome inactivation in females

· Finding potential new targets for SIRT7-mediated ADP-ribosylation upon stress

· The role of SIRT7 as a mediator of anti-aging effects in calorie-restricted mice

Bellvitge Biomedical Research Institute (IDIBELL),Dr. Alejandro Vaquero’s Lab (2013-2018)

· Characterization of SIRT7 auto‑ADP‑Ribosylation and PRC2

· Unravelling the enzymatic duality in Sirtuins

· SIRT7 in promoting genome integrity through NHEJ

· Identification of SIRT7 as a negative regulator of SirT1 during adipogenesis

· Mechanisms of SIRT7‑dependent p53 regulation in genotoxic stress

· Interplay between SIRT7 and nuclear lamina to control LINE-1 repression

Andres Bello University, Dr. Marco Alvarez’s Lab (2007-2012)

· Epigenetic regulation of rRNA and histone variant H2A.Z in carp fish

· Seasonal acclimatization and muscle plasticity through rRNA expression

· Generation of polyclonal anti-H2A.Z antibodies in rabbit (Carp antigen)

• Mentoring, Jose Manuel Castello, Ph.D. student, Josep Carreras Leukaemia Research Institute, Badalona, Spain, 01/01/22, 12/31/24
• Lecturer, Enological Biochemistry (Undergraduate course), Universidad Iberoamericana, Santiago, Chile, 01/01/12, 12/31/12
• Mentoring, Catalina Fernandez, undergraduate student, Andres Bello University, Santiago, Chile, 01/01/11, 12/31/12
• Mentoring, Paulina Rudolffi, undergraduate student, Andres Bello University, Viña del Mar, Chile, 01/01/11, 12/31/12
• Laboratory Instructor, Genetics and Molecular Biology (Undergraduate course), Andres Bello University, Santiago, Chile, 01/01/09, 12/31/11

• N. G. Simonet, J. K. Thackray, J. T. Lee, A. Vaquero, SIRT7-mediated chromatin changes on the X chromosome, FASEB: NAD + Metabolism and Signaling, 06/01/22
• N. G. Simonet, J. K. Thackray, B. N. Vazquez, A. Ianni, M. E. Alcantud, C. de la Torre, S. Hurtado, M. Olivella, M. Buschbeck, E. Bober, L. Serrano, A. Vaquero, SIRT7 is an auto-ADP-ribosyltransferase that regulates histone variant macroH2A1.1 function upon glucose starvation, Cell Symposia: Aging and Metabolism, 09/01/18
• N. G. Simonet, J. K. Thackray, B. N. Vazquez, A. Ianni, M. E. Alcantud, C. de la Torre, SS Hurtado, M. Olivella, M. Buschbeck, E. Bober, L. Serrano, A. Vaquero, SIRT7 is an auto-ADP-ribosyltransferase that regulates Polycomb function upon stress through histone variant macroH2A1.1, EMBO workshop. Histone variants: Molecular functions in health and disease, 09/01/17
• N. G. Simonet, J. K. Thackray, B. N. Vazquez, C. de la Torre, M. Olivella, E. Bober, L. Serrano, A. Vaquero, Discovering the new roles of SIRT7: the emerging Sirtuin, 3rd German-Catalan Workshop on Epigenetics, 10/01/16