Summary
Overview
Work History
Education
Skills
Websites
Publications & Scientific Contributions
Timeline
Generic

Niloufar Ziari

San Francisco,CA

Summary

Results-driven Senior Research Associate with over 5 years of experience in assay development and executing SAR-driven cell-based and biochemical assays for small molecules and monoclonal antibodies. Expertise in generating high-quality data that informs strategic planning and innovation. Strong organizational and data collection skills enhance research processes and outcomes.

Overview

5
5
years of professional experience

Work History

Senior Research Associate

RAPT Therapeutics (a subsidiary of GSK)
South San Francisco, CA
01.2026 - Current
  • Ran cell-based and biochemical assays to support SAR studies, generating and analyzing dose-response curves (EC50/EC80) using plate-based readouts (e.g., luminescence and absorbance).
  • Executed high-throughput SAR and dose-response assays using ECHO and D300 dispensing systems.
  • Designed, executed, and analyzed flow cytometry–based assays for characterization of therapeutic antibodies in clinical-stage studies.
  • Maintained and cultured diverse immune and hematopoietic cell lines, including KU812, RBL-2H3, CCRF-CEM, HEL, and SET-2 cells for experimental workflows.
  • Applied GLP principles in nonclinical experiments, including clear protocol use, accurate record-keeping, and organized data storage.
  • Research Associate II | Aug 2022 – Jan 2026
  • Research Associate I (Contract) | May 2022 – Aug 2022

Research Associate

Prosetta Biosciences
San Francisco, CA
06.2021 - 05.2022
  • Supported small-molecule discovery program investigating novel protein assembly modulators.
  • Performed SDS-PAGE and Western blotting to analyze protein expression and assess target engagement.
  • Conducted co-immunoprecipitation (Co-IP) and affinity chromatography experiments to study protein–protein interactions.
  • Prepared buffers and reagents for biochemical assays and protein purification workflows.
  • Research Intern | Sep 2020 - Jun 2021

Education

Certificate - Stem Cell & Regenerative Medicine

City College of San Francisco
San Francisco, CA
08-2020

Bachelor of Science (B.S.) - Cellular and Molecular Biology

Islamic Azad University, Science and Research Branch
Tehran, Iran
06-2013

Skills

  • Assay design and optimization
  • In vitro cell assays
  • Biochemical assays
  • Execution of chemotaxis assays
  • Assay performance optimization
  • High-throughput screening
  • Support for SAR assays
  • Quantitative dose-response assessment
  • Techniques in cell biology
  • Proficient in mammalian cell line maintenance
  • Basophil isolation techniques
  • Techniques for protein assessment
  • Western blot (SDS-PAGE)
  • Co-immunoprecipitation (Co-IP)
  • Affinity chromatography
  • Protein–protein interaction analysis
  • Experience with flow cytometry and acoustic liquid handling
  • Experience with plate-based readers
  • Analytical software skills
  • Expertise in FlowJo analysis
  • Experienced with GraphPad Prism software
  • Experience with Dotmatics platform
  • Skilled in Microsoft Office tools

Websites

Publications & Scientific Contributions

  • Lingappa AF†, Akintunde O†, Samueli E, Ewald C, Michon M, Ziari N, et al. Small molecule protein assembly modulators with pan-cancer therapeutic efficacy. Open Biology. 2024;14:240210. https://doi.org/10.1098/rsob.240210
  • Lingappa_et_al-2024-Open_Biolog...
  • Robles O
  • Brockstedt DG
  • Aponte-Guzman J, Chian D, Grandcolas M, Hu DX, Jackson JJ, Jacobson S, Karbarz E, Kassner PD, Katibah GE, Ketcham JM, Marshall L, McKinnell J, Meleza C, Milestone H, Okano A, Qiu J, Reilly MK, Riegler E, Shunatona HP, Talay O, Younai A, Ziari N, Zibinsky M, Wustrow DJ.
  • Discovery of Tivumecirnon (FLX475), a potent and selective CCR4 antagonist that modulates regulatory T-cell migration to restore antitumor immunity. Manuscript submitted for peer review.
  • Ames RY, Milestone H, Zhou F, Ziari N, Ling J, Liu P, Yu H, Guo TB, Brockstedt DG, Kassner PD. Mechanism of action of ozureprubart, a next-generation anti-IgE for the treatment of allergic diseases. Poster presented at the American Academy of Allergy, Asthma & Immunology (AAAAI) Annual Meeting

Timeline

Senior Research Associate

RAPT Therapeutics (a subsidiary of GSK)
01.2026 - Current

Research Associate

Prosetta Biosciences
06.2021 - 05.2022

Bachelor of Science (B.S.) - Cellular and Molecular Biology

Islamic Azad University, Science and Research Branch

Certificate - Stem Cell & Regenerative Medicine

City College of San Francisco

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