Nirmala Sakthivel
Canoga Park,CA
Summary
Experienced with complex scientific methodologies and research techniques. Utilizes analytical skills to drive meaningful insights and advancements. Strong understanding of experimental design and data analysis, ensuring accurate and impactful results.
Overview
5
5
years of professional experience
1
1
Certification
Work History
Clinical Genetic Molecular Biology Scientist
Quest Diagnostics
West Hills, California
08.2024 - Current
- Operated advanced laboratory equipment, including the Panther system, ensuring high precision in test execution. COBAS 8800(HIV, HCV, HPV AND COVID AND FLU)
- Verify and Validated new reagent kits work on our 13 Panthers
- Worked on Panther Link Project
- Skilled in performing CTGC/MGEN/BV/CVTV
- Performed a comprehensive range of laboratory procedures in strict adherence to established safety guidelines.
- Prepared samples and reagents for testing, meticulously following standard operating Proceduress to ensure consistency and accuracy in test results.
- Implemented quality control measures to ensure the accuracy and reliability of experimental results.
CGMBS Trainee
Kaiser Permanente
08.2023 - 08.2024
- Trained in DNA and RNA Extraction techniques using Qiagen Qiasymphony and Qiagen Qiacube systems
- Trained in utilizing Thermofisher Presto and Hamilton Microlab Star instruments for efficient extraction processes
- Trained in Next-Generation Sequencing (NGS): Specialized in NGS applications such as non-invasive prenatal testing (NIPT) and targeted gene panels for Myeloid disorders
- Trained in 3730xl DNA Analyzer for various genetic analysis including detection of Maternal cell contamination (MCC), fragile-X analysis, FLT3, PGx, HLA, JAK2 analysis for genetic variants, SMA (Spinal Muscular Atrophy), Multiplex Ligation-dependent Probe Amplification (MLPA) for copy number variation analysis, Sanger sequencing
- Genetic Testing: Skilled in performing genetic tests for TPMT, UGT1A1, Coagulation factors II and V, and MTHFR mutations
- Trained in utilizing Taqman Open Array and Cepheid BCR-ABL p210 and p190 assays for genetic analysis
- Trained in CF (Cystic Fibrosis) analysis, CMA (Chromosomal Microarray analysis)
- Trained in performing the QuantStudio 12k Flex real-time PCR for gene expression analysis
Lab Technician IV
Siemens Healthineers
Los Angeles, California
04.2022 - 07.2023
- Conducted protein purification manufacturing processes in accordance with GMP guidelines
- Optimizes manufacturing processes, work instructions, and SOPs
- Performed various protein/antibody/antigen purification techniques, including immunoaffinity, affinity, and adsorption, caprylic acid treatment
- Performed protein detection using Dot Blot and Western blot methods
- Mastered equipment such as UV detector, optical unit, spectrophotometer, centrifuges (small and large), pH meter, vacuum pump, HPLC, FPLC, gel electrophoresis, and SDS PAGE
- Utilizes AKTA-based purification methods, including affinity chromatography
- Reviews batch records and documents to ensure compliance with GDP and GMP
- Maintains laboratory documentation, logbooks, and notebooks in line with GDP standards
Lab Technician
PacGenomics Clinical Laboratory
Agoura Hills, California
08.2021 - 04.2022
- Performed COVID-19 PCR Testing: Conducted high-throughput real-time PCR tests for the detection of SARS-CoV-2, adhering to CDC and WHO guidelines
- Conducted ELISA Tests for COVID-19: Performed enzyme-linked immunosorbent assays (ELISA) to detect antibodies and antigens related to SARS-CoV-2, ensuring high accuracy and reliability
- Sample Processing: Efficiently handled and processed nasopharyngeal and oropharyngeal swab samples, ensuring accurate labeling and tracking through laboratory information management systems (LIMS)
- Data Analysis: Analyzed PCR results using specialized software, prepared detailed reports, and communicated findings to supervisors
- Troubleshooting: Identified and resolved issues related to assay performance, equipment malfunctions, and sample quality
- Participated colleagues to independently perform assays and conduct preliminary analyses
Medical Lab Technologist
Atlas Quality Assurance Laboratories
05.2021 - 07.2021
- Contributed to the development of Standard Operating Procedures (SOPs) for COVID laboratory operations and cannabis testing protocols
- Assisted in preparing documentation for submission to CLIA compliance
RNA Extraction Lab Technician
PerkinElmer
10.2020 - 03.2021
- Conducted clinical testing for SARS-CoV-2 in adherence to standard operating procedures
- Operated and troubleshooted automated microfluid systems (JANUS and CHEMAGIC) for SARS-CoV-2 clinical testing
- Executed and troubleshooted RNA extraction procedures following SOPs for SARS-CoV-2 clinical testing
- Coordinated large batches with sample accessioning and PCR analysis team departments
- Participated in training to new employees as requested
- Ensured all job duties were performed in compliance with laboratory, CLIA, and local regulations
Education
Post Baccalaureate Program - Clinical Genetic Molecular Biologist Scientist
Cal State University
Los Angeles, CA08-2024
Master of Philosophy (Research Based MS) - Biotechnology
Bharathidasan University
India08-2010
Master of Science - Biotechnology
SRM Institute of Science And Technology
India09-2006
Bachelor of Science - Microbiology
Cauvery College For Women
India06-2003
Skills
- Molecular Diagnostic Testing Techniques
- DNA/RNA Extraction
- Gel Electrophoresis
- DNA Sequencing
- Quality Control
- Data Analysis
- Laboratory Information Systems (LIS)
- Laboratory Information Management Systems (LIMS)
- Panther Link Project
Certification
- Certified Molecular Biology Technologist (MB) | American Society for Clinical
Pathology (ASCP) - CGMBS License MTP-02435438
- American Heart Association Basic Life Support (CPR and AED)
Timeline
Clinical Genetic Molecular Biology Scientist
Quest Diagnostics
08.2024 - Current
CGMBS Trainee
Kaiser Permanente
08.2023 - 08.2024
Lab Technician IV
Siemens Healthineers
04.2022 - 07.2023
Lab Technician
PacGenomics Clinical Laboratory
08.2021 - 04.2022
Medical Lab Technologist
Atlas Quality Assurance Laboratories
05.2021 - 07.2021
RNA Extraction Lab Technician
PerkinElmer
10.2020 - 03.2021
Post Baccalaureate Program - Clinical Genetic Molecular Biologist Scientist
Cal State University
Master of Philosophy (Research Based MS) - Biotechnology
Bharathidasan University
Master of Science - Biotechnology
SRM Institute of Science And Technology
Bachelor of Science - Microbiology
Cauvery College For Women
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