Paromita Das-Earl
Laboratory Manager & Research Associate
Fort Worth,TX
Summary
Highly-qualified Laboratory Manager with top-notch planning and problem-solving abilities. Open and clear communicator with disciplined and thorough approach.
Overview
13
13
years of professional experience
10
10
years of post-secondary education
Work History
Laboratory Manager
University Of North Texas HSC
Fort Worth , TX
06.2018 - Current
- Purchased laboratory equipment, chemicals and other supplies using corporate purchase card assigned
- Prepared requisition orders for research animals
- Reconciled purchase card charges and prepared expense reports monthly
- Kept equipment maintained, laboratory clean and organized, and inventory stocked to maximize performance
- Maintained fridge and freezer inventories
- Maintained animal colonies, diet schedules, drug dosing using various routes of administration
- Contacted vendors for quotes, arranged equipment repairs and payment
- Kept laboratory in compliance with applicable guidelines and laws
- Ensured adherence to good laboratory practices by lab members
- Trained new undergraduate and graduate students
- Designed and conducted experiments using various techniques like immunohistochemistry, Western Blotting, ELISA, blood pressure and glucose telemetry, behavioral assays in Zucker rat model of metabolic syndrome
- Used data analysis software such as Excel, Sigma Plot, Sigma Stat, GraphPad PrismTM, Origin, Neurolucida to analyze data generated
- Prepared graphs, statistical reports of data generated
- Written abstracts and manuscripts for submission to peer-reviewed journals
- Attended national conference and presented original research
Visiting Assistant Professor
University of Toledo
Toledo , OH
08.2010 - 05.2013
- Taught undergraduate biology courses including Diversity of Life, Evolution and Adaptation, Cell Biology and Principles of inheritance, Developmental Biology and other courses assigned by the Chair of department
- Taught undergraduate science laboratory courses, specimen preparation and dissection, use of microscopy and molecular genetic techniques
- Prepared and presented PowerPoint lectures in class sizes ranging from 100-300 students each semester
- Authored exam questions, online quizzes, and homework assignments
- Encouraged student engagement using technology such as radio frequency clickers for assessing understanding of course material
- Advised and mentored students during and outside of office hours
- Evaluated student progress, maintained detailed student records, grades and attendance
Postdoctoral Research Associate
University of Toledo Medical Center
Toledo , OH
08.2005 - 08.2010
- Designed and conducted experiments using whole-cell patch clamp, electron microscopy, molecular biology and biochemical techniques in hippocampal CA1 neurons and brain slices of a rat model of benzodiazepine withdrawal
- Maintained accurate records of research findings and provided statistical analysis of data results
- Wrote and published peer-reviewed articles
- Presented original research in national and international conferences
- Trained and managed graduate and undergraduate students in the laboratory
Postdoctoral Research Associate
Florida State University
Tallahassee , FL
03.2004 - 07.2005
- Designed and executed experiments using whole-cell patch clamp, SDS-PAGE, immunoprecipitation & PCR techniques in cultured olfactory bulb neurons from Kv1.3 knockout mice
- Analyzed data and maintained detailed scientific records
- Wrote and published manuscripts
- Presented original research in regional and national conferences.
Education
Ph.D. - Pharmacology
University of North Texas Health Science Center At Fort Worth
Fort Worth, TX 08.1998 - 12.2003
M.Sc. - Microbiology
University of Bombay
08.1995 - 07.1997
B.Sc. - Microbiology
University of Bombay
08.1992 - 07.1995
Skills
Research
Accomplishments
- Morris water maze test set up, data analysis and interpretation using lean & obese Zucker rats
- Hole-board test set up, data analysis and interpretation using lean & obese Zucker rats
- C-Fos staining, mapping and counting in fixed free floating sections from brainstem
- PhosphoTau staining in sections from brainstem and hippocampus
- Estrogen receptor alpha and G-Protein Estrogen Receptor (GPER) staining in forebrain
- Whole-cell patch clamp in transfected human embryonic kidney (HEK-293T) cells
- Whole-cell patch clamp in primary hippocampal neuronal culture and acutely isolated hippocampal CA1 neurons
- Cell-attached GABAR single-channel recordings from acutely isolated hippocampal neurons
- Electron Microscopy
- Transcardial perfusion, rat brain harvest and sectioning
- Ultramicrotomy
- Pre and post embedding immunogold labeling
- Analyzing samples under standard transmission electron microscope
- Site-directed mutagenesis
- Recombinant DNA techniques- developed stable cell lines expressing mouse 5-HT3A receptor
- Sub cloning
- Agarose gel electrophoresis
- PCR
- DNA and RNA isolation
- SDS-PAGE, immunoblotting, quantitative densitometry
- ELISA
- Immunoprecipitation
- Cell Culture
- Primary hippocampal and olfactory bulb cell culture
- HEK-293T culture and maintenance
- Transfection using Ca-phosphate precipitation and LipofectamineTM
- Microbiology
- Culturing and aseptic techniques, microbiological media preparation
- Animal Handling
- Rat and mouse colony maintenance, intraperitoneal injections, tail glucose sampling, dosing with various drugs
Publications
1. Chaudhary P., Das-Earl P, and Schreihofer AM. Preserved glycemic control and baroreflex efficacy in young adult hypertensive female obese Zucker rats. Am J Physiol Regul Integr Comp Physiol 2021 May 12. . doi: 10.1152/ajpregu.00341.2020.
2. Earl DE, Das P, Gunning III WT, and Tietz EI. . Regulation of Ca2+/calmodulin-dependent protein kinase II signaling within hippocampal glutamatergic postsynapses during flurazepam withdrawal. Maladaptive Plasticity in Neurological Diseases. Special Issue of Neural Plasticity, 2012:405926. doi: 10.1155/2012/405926. Epub 2012
3. Das P., Zerda R., Alvarez F.J. and Tietz E.I. Immunogold electron microscopic evidence of differential regulation of GluN1, GluN2A and GluN2B, NMDA-type glutamate receptor subunits in rat hippocampal synapses during benzodiazepine withdrawal. J. Comp. Neurol., 518(21):4311-4328, 2010
4. Lu S., Das P., Fadool D.A., and Kaczmarek L.K. The slack sodium-activated potassium channel provides a major outward current in olfactory neurons of Kv1.3-/- super-smeller mice. J. Neurophysiol., 103: 3311-3319, 2010.
5. Shen G., Mohammed M.S., Das P., and Tietz E.I. Positive allosteric activation of GABAA receptors bi-directionally modulates hippocampal glutamate plasticity and behaviour. Biochem Soc Trans 37 (Pt 6):1394-1398, 2009.
6. Das P., Lilly S.M., Zerda R, Gunning III W.T., Alvarez F.J. and Tietz E.I. Increased AMPA receptor GluR1 subunit incorporation in rat hippocampal CA1 synapses during benzodiazepine withdrawal. J. Comp. Neurol., 511, 832-846, 2008.
7. Das P., Parson A.D., Scarborough J., Hoffman J., Wilson J., Thompson R.N., Overton J.M., and Fadool D.A. Electrophysiological and behavioral phenotype of insulin receptor defective mice. Physiology and Behavior, 86, 287-296, 2005.
8. Das P., and Dillon G.H. Molecular determinants of picrotoxin inhibition of 5-Hydroxytryptamine type 3 receptor. J. Pharmacol. And Exp Thera. 314 (1), 320-328, 2005.
9. Das P., Bell-Horner C.L., Huang R.Q., Raut A., Gonzales E.B., Covey D.F., and Dillon G.H. Inhibition of recombinant GABAA receptors by L-type calcium channel blockers. Neurosci., 124(1), 195-206, 2004.
10. Das P., and Dillon G.H. The 5-HT3B subunit reduces picrotoxin sensitivity when co- expressed with the 5-HT3A receptor. Mol. Brain. Res., 119 (2), 207-212, 2003.
11. Das P., Bell-Horner C.L., Machu T.K., and Dillon G.H. The GABAA receptor antagonist picrotoxin inhibits 5-Hydroxytryptamine type 3A receptors. Neuropharmacol., 44, 431-438, 2003.
Timeline
Laboratory Manager
University Of North Texas HSC
06.2018 - Current
Visiting Assistant Professor
University of Toledo
08.2010 - 05.2013
Postdoctoral Research Associate
University of Toledo Medical Center
08.2005 - 08.2010
Postdoctoral Research Associate
Florida State University
03.2004 - 07.2005
Ph.D. - Pharmacology
University of North Texas Health Science Center At Fort Worth
08.1998 - 12.2003
M.Sc. - Microbiology
University of Bombay
08.1995 - 07.1997
B.Sc. - Microbiology
University of Bombay
08.1992 - 07.1995
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