Paulinus Orenie Nwabia
Newark,DE
Summary
Detail-oriented Research Assistant with hands-on experience performing clerical and laboratory work to advance research goals. Drives team success with expertise in data review, reporting and specimen handling. Specializes in research and exploration of mycobacterial stress responses pathways.
Skilled in collaborating effectively with colleagues and principal investigators to improve experimental procedures. Demonstrated ability to troubleshoot and test to enhance data analysis accuracy.
Overview
9
9
years of professional experience
Work History
Research Assistant (Graduate Level)
University of Delaware
06.2022 - 05.2025
- Designed and optimized stress-response molecular assays using both quantitative PCR (qPCR) and fluorescent reporters in Mycobacterium smegmatis, achieving greater than 30% improved sensitivity.
- Conducted RNA-seq experiments to map transcriptional changes under antibiotic pressure and performed differential expression analysis using DESeq2.
- Led cross-functional coordination to ensure data integrity, reproducibility, and standardized reporting.
- Maintained full compliance with BSL-2 regulations and supported lab GLP-style documentation.
Graduate Teaching Assistant
University of Delaware
02.2022 - 02.2025
- Supervised undergraduate labs (50+ students), emphasizing aseptic technique, molecular protocols, lab safety, and accurate data recording.
- Developed training materials and checklists to ensure compliance with lab SOPs and quality standards.
On-field Research Officer
Rivers State, Nigeria
09.2016 - 07.2018
- Led on-site implementation of poultry genetic improvement research across 12 research stations, covering 420 smallholder farm families, increasing enrollment by 25%.
- Supervised field trials evaluating genetic gains in seven poultry strains, ensuring adherence to experimental protocols.
- Collected, validated, and analyzed large-scale phenotypic data to assess breeding performance and inform strain selection strategies. Achieved above 98% clean data before analysis with a data collection efficiency of 87%.
- Facilitated stakeholder engagement through regular data review sessions and innovation meetings, promoting collaborative decision-making and continuous improvement in breeding programs, improving stakeholders' and value chain actors' confidence by 25% in 4 months.
Education
M.S. - Molecular Biology and Genetics
University of Delaware
Newark, DE05.2025
B.S. - Animal Sciences
University of Port Harcourt
Choba, RV05.2015
Skills
- RNA-seq data analysis
- Alignment tools
- Basic DESeq2
- QPCR assay design
- Validation
- Troubleshooting
- Protein purification
- Western blotting
- BSL-2 level aseptic procedures
- BSL-2 level safety procedures
- Sample tracking
- Laboratory ELN documentation
- CLIA compliance
- GLP compliance
- Quality control
Timeline
Research Assistant (Graduate Level)
University of Delaware
06.2022 - 05.2025
Graduate Teaching Assistant
University of Delaware
02.2022 - 02.2025
On-field Research Officer
Rivers State, Nigeria
09.2016 - 07.2018
M.S. - Molecular Biology and Genetics
University of Delaware
B.S. - Animal Sciences
University of Port Harcourt
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