Summary
Overview
Work History
Education
Skills
Affiliations
EXTRA CURRICULAR ACTIVITIES
Languages
Interests
Timeline
Poster Presentations/Meetings
Selected Publications/Interview/Patent
Generic
Pharvendra Kumar

Pharvendra Kumar

Worcester,USA

Summary

Post-Doctoral Associate with expertise in DNA sequencing microbial assays and Anaerobic culture at UMass Chan Medical School. Leading a project's aim to reduce health outcomes of Klebsiella pneumoniae infection in mice models by testing innovative therapeutic interventions like synthetic microbial consortium.

Former doctoral Researcher at Regional Centre for Biotechnology India, experienced in host-pathogen interaction research, methods in molecular biology, cellular immunology, microbiology and computational biology and mice models. Core competencies include creative thinking, collaborative teamwork, and strategic planning. Career goal: advancing biologics solutions through cutting-edge research methodologies. Detail-oriented Research Fellow with more than 8th years of substantial experience in sophisticated research techniques and technologies in Biotechnology field. Organised and able to take charge.


Overview

14
14
years of professional experience

Work History

Post-Doctoral Associate

UMass Chan Medical School
01.2024 - Current
  • In Vanni Bucci lab I am investigating the detailed potential role of modulated gut microbiota as therapy in different pathogen infections and inflammatory colitis disease.
  • Lead independent research project, I established klebsiella colonization mice model to reduce klebsiella mediated inflammation and abundance/ decolonization in the gut to lower risk outcomes for attain short and long-term objectives.
  • Expert in Colony Forming Unit from different organs, stool, bacterial culture and cell lines.
  • Designed research projects and alternative approaches and discussed results with professor.
  • Collaborated with multidisciplinary team members to accomplish their research goals.
  • Maintained accurate records of research findings and provided statistical analysis of data results.
  • Contributed to and actively participated in research conception, design and execution to address defined problems.
  • Reviewed manuscripts for esteemed scientific journals, contributing to the advancement of knowledge within the field.
  • Experienced in DNA sequencing, ELISA and Anaerobic culture.
  • Expert in mice handling, Breeding, Genotyping, Genetic (TNF-a) and induced Inflammatory (Klebsiella, C.diff) mice model, Mice dosage (IP, Gavage), Blood and Stool collection.

Senior Research Fellow (ICMR)

Regional Centre for Biotechnology
03.2021 - 12.2023
  • During my PhD I also worked independently as Senior Research Fellow in Prof. C V Srikanth lab. I was Investigating of the regulatory mechanisms controlling SUMO pathway genes during Salmonella infection and inflammation using molecular, cellular and biochemical approaches.
  • Mentored junior researchers, providing guidance on study design and data analysis techniques for successful project outcomes.
  • Won research fellowship from Indian Council of Medical Research (ICMR) to work as Senior Research Fellow.
  • Worked on Gene or promoter cloning, vector designing, In-Silico promoter analysis, bacterial transformation, Site directed mutagenesis
  • Experienced in Mammalian cell lines/primary cells/intestinal organoid culture.
  • Expert in PCR, qRT-PCR, western blotting, co-immunoprecipitation.
  • Experienced in Chromatin immunoprecipitation assay, Electrophoretic mobility shifty assay, Wound healing assay, protein-protein network analysis.
  • Expert in Sub-cellular fractionation in mammalian cells.

Ph.D.

Regional Centre for Biotechnology
08.2014 - 06.2023
  • I completed PhD under supervision of Prof. C V Srikanth. My role was Identified regulation of SUMO pathway genes and modulation of inflammatory signaling during Salmonella Typhimurium infection and intestinal inflammation.
  • Self-motivated, with a strong sense of personal responsibility.
  • Skilled at working independently and collaboratively in a team environment.
  • Proven ability to learn quickly and adapt to new situations.
  • A strong communicator and team leader or co-leader of collaborative projects with other labs in India.
  • Mentored students in the laboratory to support successful completion of their master’s degrees under my guidance.
  • Assisted master’s students in conducting laboratory practical and hands-on skills.
  • Published peer reviewed journals in Journal of Cell Science, Pathology & Oncology Research.
  • Experienced in Confocal and fluorescence microscopy, Flow cytometry.
  • Expert in mice handling, Salmonella induced Inflammatory mice model, Tumor isolation, Mice dosage (Gavage), Dissection and organs isolation, H&E staining.
  • Expert in Si-RNA, plasmids transfection in vitro and in vivo methods.
  • Experienced in mRNA sequencing, MTT assay, colony forming unit.

Junior Research Fellow

Institute of Pesticide Formulation Technology
09.2013 - 07.2014
  • I worked as Junior Research Fellow with Dr. Amrish Agrawal at Institute of Pesticide Formulation Technology, gurugram, India. I worked on a project "Developed user & environment friendly water dispersible granule of broad-spectrum pesticide to reduce their toxicity for continuation of use".
  • Strengthened project outcomes by employing rigorous quality control measures throughout all phases of experimentation.
  • Worked as member of integrated project team in highly collaborative work environment.
  • Project findings was patented in US Patent 1682/DEL/2014 Institute of Pesticide Formulation Technology (IPFT), Gurugram, India.

M.Sc. Dissertation Project

Institute of Genomics and Integrative Biology
01.2012 - 05.2012


  • I worked as trainee under supervision of Dr. Bhupesh Taneja. I expressed and purify Mycobacterial Topoisomerase-I (Topo-I) protein from Mycobacterium smegmatis and E.coli.

Education

Ph.D. - Biotechnology

Regional Centre For Biotechnology
01.2023

M.Sc. - Biotechnology

Jamia Millia Islamia
01.2012

B.Sc (H) - Biochemistry

Bundelkhand University
01.2009

Skills

  • Collaborative research
  • Scientific communication
  • Lab management
  • Project management
  • Research design
  • Manuscript preparation
  • Time management abilities
  • Analytical thinking
  • Organizational skills
  • Multitasking Abilities
  • Teamwork and collaboration
  • Proficient in GraphPad Prism, ImageJ, Gene Runner, Mat inspector, CiiiDER

Affiliations

  • Reviewer, Molecular Neurobiology Journal (Springer Nature)

EXTRA CURRICULAR ACTIVITIES


  • Have won One Silver medal in Badminton tournament at RCB annual sports meet (2023)
  • Attended Online course on Flow Cytometry organized by Flowcytometry solutions (p) Ltd, Jaipur, India (2022)
  • Participated in Intellectual Property Awareness / Training program under National Intellectual Property Awareness Mission (2022)
  • Have won Two silver and One Bronze medals in Badminton tournament at RCB annual sports event (2019)
  • “Certificate of Excellence” by The central railway employees co-operative credit society ltd., Byculla, Mumbai (2006)
  • Passed Certificate “A” examination under the authority of Ministry of Defense, GOI (2004)
  • Participated in Combined Annual Training Camp of National Cadet Corps (NCC) in 3 UP Air Sqn NCC Kanpur Unit (2002)

Languages

English
Professional Working
Hindi
Professional Working

Interests

Love playing Badminton, cricket and tennis during free time

I enjoy helping others and giving back to the community

Music

Volunteer Work

Timeline

Post-Doctoral Associate

UMass Chan Medical School
01.2024 - Current

Senior Research Fellow (ICMR)

Regional Centre for Biotechnology
03.2021 - 12.2023

Ph.D.

Regional Centre for Biotechnology
08.2014 - 06.2023

Junior Research Fellow

Institute of Pesticide Formulation Technology
09.2013 - 07.2014

M.Sc. Dissertation Project

Institute of Genomics and Integrative Biology
01.2012 - 05.2012

Ph.D. - Biotechnology

Regional Centre For Biotechnology

M.Sc. - Biotechnology

Jamia Millia Islamia

B.Sc (H) - Biochemistry

Bundelkhand University

Poster Presentations/Meetings

  • Poster presented at RCB programme advisory committee (PAC-2023) RCB, Faridabad titled “PTMs mediated selective regulation of target genes by Activator protein 1 (AP-1): Implication in Host-Salmonella interaction” (2023)
  • Poster presented at NCR-Cluster Young Investigator’s Symposium, RCB, Faridabad titled “Salmonella Typhimurium governs selective regulation of UBC9 and PIAS1 by Activator protein 1 (AP1)” (2019)
  • Poster presented at International Symposium on Infectious Diseases (ID-Symposium), Jamia Hamdard, Delhi NCR titled “A PTM dependent selective regulation of UBC9 and PIAS1 by Activator protein 1 (AP1) during Salmonella Typhimurium infection” (2018)
  • Poster presented at National institute of advanced industrial science and technology (AIST), Japan titled “SUMOylation dependent selective regulation of AP-1 target genes during Salmonella Typhimurium infection” (2018)
  • Poster presented at RCB programme advisory committee (PAC-2017) RCB, Faridabad titled “A novel SUMOylation dependent regulation of UBC9 and PIAS1 by AP-1 transcription factor during Salmonella infection” (2017)
  • Poster presented at RCB programme advisory committee (PAC-2016) RCB, Faridabad titled “Understanding the Regulation of SUMO pathway genes during Salmonella Typhimurium infection and intestinal inflammation” (2016)
  • Joint IUBMB-RCB Advanced School-2014 “Diabetes and Metabolic Syndrome Networks, Crosstalk and Interventions”, Manesar, Haryana, India (2014)

Selected Publications/Interview/Patent

1. Kumar, P., Soory, A., Mustfa, S. A., Sarmah, D. T., Devvanshi, H., Chatterjee, S., Bossis, G., Ratnaparkhi, G. S. and Srikanth,

C. V. Bidirectional regulation between AP-1 and SUMOylation pathway genes modulates inflammatory signaling during

Salmonella infection. J Cell Sci (2022) 135 (16): jcs260096. https://doi.org/10.1242/jcs.260096.

2. Awasthi, A., Kumar, P., Srikanth, C.V. et al. In vitro Evaluation of Torin2 and 2, 6-Dihydroxyacetophenone in Colorectal

Cancer Therapy. Pathol. Oncol. Res. 25, 301–309 (2019). https://doi.org/10.1007/s12253-017-0347-7

3. Amrish Agrawal, Dipak Kumar Hazra, Pharvendra Kumar, Phool Kumar Patanjali, S.K. Raza “Water dispersable Granule

Formulation of Insecticide TRIAZOPHOS” - US Patent 1682/DEL/2014 Institute of Pesticide Formulation Technology (IPFT),

Gurugram, India.

4. First person – Pharvendra Kumar. Bidirectional regulation between AP-1 and SUMOylation pathway genes modulates

inflammatory signaling during Salmonella infection. J Cell Sci (2022) 135 (16): jcs260486.

https://doi.org/10.1242/jcs.260486. Online Issn: 1477-9137. Print Issn: 0021-9533

5. Salman Ahmad Mustfa, Pharvendra Kumar, Dikshalee Bassi, Aamir Suhail, Neha Yadav, Chirantan Debnath, Jitendra

Yadav, Ayub Qadri, Pallavi Kshetrapal, and Chittur V. Srikanth, “PIAS1-BIRC3 axis at mucosal interface restricts Salmonella

Typhimurium colonization and migration” (Under Communication)