Rafaela Sartore

Stem cell scientist with more than 15 years of laboratory experience in human pluripotent stem cells and stem cell-based models for studying neural development, cell fate specification, and disease modeling. Actively leads the development and execution of new experimental approaches to advance stem cell models in Neuroscience, including 3D organoid cultures. Extensive experience in working independently, managing, overseeing, and executing cutting-edge research projects that somehow require creative solutions. Collaborative work builder with intern and extern partners resulting in 18 peer-reviewed scientific publications, among other projects. A well-organized, detail-oriented, and team-focused individual with proven success in effectively working within multifunctional and multidisciplinary teams in a diverse, complex, and fast-paced scientific environment. Highly motivated and enthusiastic about continuous learning, with a demonstrated willingness to adapt to emerging scientific advancements.

• Best Doctoral Thesis Award of Morphological Sciences Program, Federal University of Rio de Janeiro, 2016
• Juarez Aranha Ricardo Award, Brazilian Society of Neurosciences and Behavior, 2011
• Young Investigator Award, Brazilian Society of Cell Biology, 2010
• Honorable Mention of the Science and Technology Incentive Award, Ministry of Health, 2009
• Honorable Mention Prize Ricardo Ribeiro dos Santos, III International Symposium of Cell Therapy, 2008
• Honorable Mention, XXII Annual Meeting of the Federation of Societies of Experimental Biology, 2007

Master in Applied Sciences to the Musculoskeletal System, National Institute of Traumatology and Orthopaedics, Rio de Janeiro, Brazil

Rhayra Braga Dias, Isolation and expansion of human bone marrow stromal cells under GMP conditions following Brazilian Regulatory Agency norms to be used in clinical protocols, 2017

Gustavo Borges de Azevedo, Evaluation of the osteogenic potential of human bone marrow stromal cells isolated from patients with congenital and idiopathic scoliosis, 2018

Breno Scorza, Establishment and characterization of the Masquelet induced membrane technique in a rat femur critical-sized defect model, 2018

• Cell-type specific defects in PTEN-mutant cortical organoids converge on abnormal circuit activity, Pigoni M, Uzquiano A, Paulsen B, Kedaigle A, Yang SM, Symvoulidis P, Adiconis X, Velasco S, Sartore R, Kim K, Tucewicz A, Tsafou K, Jin X, Barrett L, Chen F, Boyden E, Regev A, Levin JZ, Arlotta P., Hum Mol Genet, 10.1093/hmg/ddad107, 2023
• Autism genes converge on asynchronous development of shared neuron classes, Paulsen B, Velasco S, Kedaigle A, Pigoni M, Quadrato G, Deo A, Adiconis X, Uzquiano A, Sartore R, Yang SM, Simmons S, Symvoulidis P, Kim, K Tsafou K, Podury A, Abbate C, Tucewicz A, Smith S, Albanese A, Barrett L, Sanjana NE, Shi X, Chung K, Lage K, Boyden E, Regev A, Levin JZ, Arlotta P., Nature, 602, 7896, 268-273, 10.1038/s41586-021-04358-6, 2022
• PTH1-34 improves devitalized allogenic bone graft healing in a murine femoral critical size defect, Rocha T, Cavalcanti AS, Leal AC, Dias RB, Sartore RC, Ribeiro GO, Guimarães JAM, Duarte MEL., Injury, 10.1016/j.injury.2021.03.063, 2021
• Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities, Nascimento JM, Saia-Cereda VM, Sartore RC, da Costa RM, Schitine C, Freitas HR, Murgu M, de Melo Reis RA, Rehen SK, Martins-de-Souza D., Front Cell Dev Biol, 7, 303, 10.3389/fcell.2019.00303, 2019
• The Manufacture of GMP-Grade Bone Marrow Stromal Cells with Validated In Vivo Bone-Forming Potential in an Orthopedic Clinical Center in Brazil, Dias RB, Guimarães JM, Cury MB, Rocha LR, da Costa ES, Nogueira LP, Hochman-Mendez C, Fortuna-Costa A, Silva AKF, Cunha KS, de Souza SAL, Duarte MEL, Sartore RC, Bonfim DC., Stem Cells International, 2019, 2608482, 2019
• Osteoarthritic Synovial Fluid Modulates Cell Phenotype and Metabolic Behavior In Vitro, de Sousa EB, Dos Santos Junior GC, Aguiar RP, da Costa Sartore R, de Oliveira ACL, Almeida FCL, Neto VM, Aguiar DP., Stem Cells International, 2019, 8169172, 2019
• Atrophic nonunion stromal cells form bone and recreate the bone marrow environment in vivo, Vallim F, Guimaraes JM, Dias RB, Sartore RC, Cavalcanti AS, Oliveira ACL, Duarte MEL, Bonfim DC., OTA International, OTA Int, 1(3): e008, 2018
• Short term changes in the proteome of human cerebral organoids induced by 5-methoxy-N,N-dimethyltryptamine, Dakic V, Nascimento JM, Sartore RC, Maciel RM, de Araujo DB, Ribeiro S, Martins-de-Souza D, Rehen SK., Sci Rep, 7, 12863, 2017
• Derivation of functional human astrocytes from cerebral organoids, Dezonne RS, Sartore RC, Minardi JN, Saia-Cereda VM, Romão LF, Alves-Leon SV, Marcondes de Souza J, Martins-de-Souza D, Rehen SK, Gomes FCA., Sci Rep, 7, 45091, 2017
• Trace elements during primordial plexiform network formation in human cerebral organoids, Sartore RC, Cardoso SC, Lages YM, Paraguassu JM, Stelling MP, Madeiro da Costa R, Guimarães MZP, Pérez CA, Rehen SK., PeerJ, 5, e2927, 2017
• Bone intramedullary reaming grafts the fracture site with CD146 + skeletal progenitors and downmodulates the inflammatory environment, Rocha LR, Sartore RC, Leal AC, Dias RB, Duarte MEL, Guimarães JAM, Bonfim DC., Injury, 48, S41-S49, 2017
• Vertical femoral neck fractures in young adults: a closed fixation strategy using a transverse cancellous lag screw, Guimarães JAM, Rocha LR, Noronha Rocha TH, Bonfim DC, Sartore RC, Dos Santos Cavalcanti A, Roesler CR, Perini JA, Aguiar DP, Duarte MEL., Injury, 48, S10-S16, 2017
• Cycle arrest and aneuploidy induced by zidovudine in murine embryonic stem cells, Campos PB, Sartore RC, Ramalho BL, Costa ES, Rehen SK., Mutagenesis, 27, 431-436, 2012
• Implications of aneuploidy for stem cell biology and brain therapeutics, Devalle S, Sartore RC, Paulsen BS, Borges HL, Martins RAP, Rehen, S.K., Frontiers in Cellular Neuroscience, 6, 1-2, 2012
• Retinoic acid-treated pluripotent stem cells undergoing neurogenesis present increased aneuploidy and micronuclei formation, Sartore RC, Campos, PB, Trujillo, CA, Ramalho BL, Negraes PD, Paulsen, BS, Meletti T, Costa ES, Chicaybam L, Bonamino MH, Ulrich H, Rehen SK., Plos One, 6, e20667, 2011
• Cannabinoids modulate cell survival in embryoid bodies, Nones J, Spohr TCLS, Furtado DR, Sartore RC, Paulsen BS, Guimarães MZP, Rehen SK., Cell Biology International, 34, 399-408, 2010
• Chromosomal spread preparation of human embryonic stem cells for karyotyping, Campos Priscila B, Sartore RC, Abdalla SN, Rehen SK., J Vis Exp, pii: 1512, 2009
• Successful scale-up of human embryonic stem cell production in a stirred microcarrier culture system, Fernandes AM, Marinho PAN, Sartore RC, Paulsen BS, Mariante RM, Castilho LR, Rehen SK., Braz J Med and Biol Res, 42, 515-522, 2009

Aneuploidy in Embryonic Stem Cells, Stem cells: From tools for studying mechanism of neuronal differentiation towards therapy, NY : Springer, 2009