Robert Amongre
Davis,CA
Summary
Experienced professional scientist with a strong background in analytical chemistry, organic chemistry, and protein science. Skilled in method development, data interpretation, and instrument maintenance. Proven track record of driving research projects forward with precision and innovation, while fostering a collaborative team environment. Recognized for adaptability, problem-solving skills, and consistently delivering impactful results. Proficient in chromatography, spectroscopy, and various analytical techniques.
Overview
7
7
years of professional experience
Work History
Analytical Research Chemist
Nivagen Pharmaceuticals
01.2025 - Current
- Performed extraction, analysis, and characterization of extractable and leachable from container closure systems of pharmaceutical drug products as per PQRI, ICH and other regulatory guidelines
- Provided guidance during regulatory inspections, effectively addressing questions related to method validation and data quality control procedures.
- Developed and validated HPLC, LC-MS, GC-MS and ICP-MS methods for the characterization of finished product, raw material
- Prepared and reviewed analytical methods, method validation and related study reports for ANDA/ NDA submissions and support, document preparations for ANDA/ NDA submissions
- Maintained, Operated and troubleshot LC-MS and ICP-MS Systems
Research Associate, Protein Science
Genentech
10.2023 - 12.2024
- Troubleshot and optimized protein purification methods to improve the quality and yields of proteins including virus-like particles (VLPs) and protein vaults for multiple projects
- Designed and executed binding methods to effectively characterize the kinetic parameters of VLPs to their target receptors
- Collaborated with cross-functional teams on maintenance of instrumentation and design and execution of experiments
- Ensured that ELNs are updated with completed and new experiment for proper records and easy reference
- Designed, optimized, and applied various chemistries to effectively achieve targeted conjugation of different target specific peptide moieties to viral-like particles (VLPs) in support of capsid engineering objectives
Research Associate, Protein – Protein Interactions
University of California
07.2020 - 09.2023
- Independently designed and optimized in vitro biochemical functional assays using Electro-spray UPLC to profile small molecule ATP competitive inhibitors for the D1 domain of p97/VCP
- Designed novel synthetic routes to synthesize and biochemically evaluate ATP inhibitors as chemical probes for PPIs associated with p97
- Optimized multi-route purification steps resulting in scale up yield of radio-tracer 18F-TRX by 5-fold
- Troubleshot and executed protocols to synthesize various recombinant protein from E Coli from cloning, initial expression, scaled up purification, and characterization to support multiple projects
- Designed and optimized binding assays to achieve the kinetic and affinity properties of PPIs with VCP
- Ensured that ELN is updated with completed and new experiment for proper records and easy reference (Signal Notebook)
Graduate Research, Bioelectrochemistry
San Francisco State University
08.2018 - 06.2020
- Designed, developed and validated a novel spectro-electrochemical system to evaluate the electroenzymatic epoxidation of styrene that resulted in the identification of metallic copper working electrode which exponentially improved Flavin adenine dinucleotide (FAD) reduction and styrene epoxidation efficiency
- Designed and optimized relevant experimental methods to characterize the yields of electroenzymatic FAD reduction and styrene epoxidation
- Optimized protocol to increase the yield of purified styrene monooxygenase for project needs
- Led and supervised undergraduate students through seminar discussion, lab work, and office hours
Education
MSc. - Chemistry/Biochemistry
San Francisco State University
San Francisco, CA06.2020
BSc. - Chemistry
Kwame Nkrumah University of Science And Technology
Ashanti Region, Ghana06.2010
Skills
- Collaborative teamwork
- Method development
- Analytical problem solving
- Quality control procedures
Accomplishments
- Achieved high quality proteins by optimizing methods for purification.
- Used google spreadsheet to develop inventory tracking systems for multiple project deliveries.
- Collaborated with external companies to streamline the purification system for AAVs and VLPs.
Relevant Technical Skills
- Biochemical Assays - BCA protein assay, Bradford assay, Biomol green assay, protein unfoldase assay, FI, FP, Immunoprecipitation, co-immunoprecipitation, SPR, ITC, BLI, DSF, DLS, MST
- Protein Purification and Characterization - Gel electrophoresis, SEC, IMAC, IEC, SDS – Page, PCR, Flow cytometry, Cell culture, HPLC, LC-MS, AKTA system, MS
- Analysis and Quantification - GraphPad Prism, Microsoft excel, R programming
- Structure Visualizations – Pymol, Chimera, Genius prime, ChimeraX
- Cloning (DNA/Plasmid Designs) – PCR, Benchling
- Purification (Flash Chromatography, TLC, Prep HPLC, Distillation, Crystallization)
- Characterization (GC/MS, LC/MS, MS, Analytical HPLC, NMR, FT/IR)
- Literature Tools (Scifinder, Reaxys, BioPharm, ChemPlanner, SYNTHIATM, Retrosyntheis)
- Computational (Schrödinger, Virtual Screen, Docking, Cytoscape)
- Data Storage and Presentation (Jupiter notebook, MS PowerPoint, signal notebook)
- Structure & Others (Quartzy, ChemDraw, Data warrior, Mybuy, smartbuy)
Publications
Amongre, Robert & Gassner, George, 2021, Regenerable copper anode for the Cu (I)-mediated reduction of FAD in the electroenzymatic styrene epoxidation reaction, Bioelectrochemistry, 137, 107679, 10.1016/j.bioelechem.2020.107679
Presentations
- 10/2019, Electrochemical Reduction of FAD for NSMOA‐Catalyzed Epoxidation Reactions, Honolulu, Poster
- 11/2019, Electrochemical Reduction of FAD for NSMOA‐Catalyzed Epoxidation Reactions, Anaheim, Poster
- 04/2020, Regenerable copper anode for the Cu (I)-mediated reduction of FAD in the Electroenzymatic styrene epoxidation reaction, Virtual, Poster
- 11/2022, Probing the Effects of Conformational dynamics on the protein – protein interactions of Valosin containing protein (VCP)/p97, Santa Cruz, Poster
Languages
English
Full Professional
French
Elementary
Timeline
Analytical Research Chemist
Nivagen Pharmaceuticals
01.2025 - Current
Research Associate, Protein Science
Genentech
10.2023 - 12.2024
Research Associate, Protein – Protein Interactions
University of California
07.2020 - 09.2023
Graduate Research, Bioelectrochemistry
San Francisco State University
08.2018 - 06.2020
MSc. - Chemistry/Biochemistry
San Francisco State University
BSc. - Chemistry
Kwame Nkrumah University of Science And Technology
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