Summary
Overview
Work History
Education
Skills
Accomplishments
References
Timeline
Generic

Ruby Maharjan

Malden,MA

Summary

Experienced researcher with extensive expertise in advanced cell culture, including CRISPR techniques, assay development, flowcytometry, imaging analysis and immunohistochemistry. Proficient in animal handling and in vivo models, with a strong background in molecular biology, immunology, and tumor microenvironment. Experienced in formulation of nanoparticles and ADC including study of intracellular trafficking with background in targeted drug design and delivery. Skilled in a range of biopsy, tissue processing, and protein/DNA/RNA analysis techniques. Highly adept in various analytical software tools, ensuring precise data analysis and effective scientific communication. Published number of first author and co-author original research paper in peer reviewed journals. Additionally, 4 years of professional experience in pharmaceutical industry.

Overview

15
15
years of professional experience

Work History

Postdoctoral Fellow

Mass General Hospital
Boston, MA
02.2023 - Current
  • Leading two projects on study of TNBC tumor environment correlating with racial disparity and study of TP53 promoter region mutation with aim of genetic and epigenetic profiling.
  • Developed protocols for DNA/RNA extraction from patient biopsy, cell isolation for scRNA sequencing and 10x scRNA library preparation.
  • Trained and mentored undergraduate students on various molecular biological techniques and projects.

Postdoctoral Scholar

University of Kentucky
Lexington, KY
05.2022 - 01.2023
  • Worked on modulation of immune system using nanoparticles in cancer and spinal cord injury.
  • Developed mouse model for spinal cord injury and developed protocol for cell isolation from spinal cord for flowcytometry.
  • Trained and mentored graduate and undergraduate students on mouse handling, various molecular biological techniques, and projects.

PhD Scholar

Seoul National University
Seoul, South Korea
09.2016 - 07.2021
  • Discovered anti-angiogenesis effect of metronomic chemotherapy in cancer model.
  • Discovered modulation of immune system by metronomic chemotherapy and immunotherapy by inducing immunogenic cell death.
  • Developed and characterized nanoparticles for oral administration with pharmacokinetics and efficacy study.
  • Discovered a new biomarker for cancer and developed antibody-drug conjugate along with screening of antibodies, studying intracellular trafficking.
  • Developed a liver metastasis model for colorectal cancer and found the efficacy of metronomic drug delivery for the inhibition of metastasis.
  • Trained and mentored graduate and undergraduate students on various molecular biological techniques, mouse handling, and projects.

Quality Control Incharge

QMED Formulation Pvt. Ltd
Bhaktapur, Nepal
07.2013 - 08.2015
  • Acceptance or rejection of products, raw materials, and packaging materials
  • documentation of Standard Operating Procedures, Standard Testing Protocols, and other protocols according to WHO-GMP
  • method development and validation of pharmaceutical products; analysis using HPLC, IR, and UV; and microbiological assay

Quality Control Pharmacist

Simca Laboratories Pvt. Ltd.
Bhaktapur, Nepal
06.2010 - 01.2012
  • Analysis of raw materials, packaging materials and finished products
  • Line clearance, Dispensing, and Documenting
  • Analysis using HPLC and UV

Education

Ph.D. - Pharmacy

Seoul National University
Seoul, South Korea
02-2021

Master of Science - Pharmaceutical Sciences

Kathmandu University
Kathmandu, Nepal
12-2013

Bachelor of Science - Pharmacy

Purbanchal University
Lalitpur, Nepal
06-2010

Skills

  • Molecular techniques
  • Biochemistry techniques
  • DNA/RNA extraction/assay
  • qPCR/ddPCR
  • Cell isolation from tissue
  • Immunohistochemistry
  • Flow cytometry
  • Animal model development
  • In vivo imaging
  • Pharmacokinetics
  • Nano-formulation
  • Project management
  • Research design

Accomplishments

  • Korean Government Scholarship for PhD, 2015-2018
  • Nepal Student Bidhya Medal from President of Nepal, 2010 (Best student award)
  • Scholarship for Higher Education from Embassy of India in Nepal, 2002-2004

References

References available upon request.

Timeline

Postdoctoral Fellow

Mass General Hospital
02.2023 - Current

Postdoctoral Scholar

University of Kentucky
05.2022 - 01.2023

PhD Scholar

Seoul National University
09.2016 - 07.2021

Quality Control Incharge

QMED Formulation Pvt. Ltd
07.2013 - 08.2015

Quality Control Pharmacist

Simca Laboratories Pvt. Ltd.
06.2010 - 01.2012

Ph.D. - Pharmacy

Seoul National University

Master of Science - Pharmaceutical Sciences

Kathmandu University

Bachelor of Science - Pharmacy

Purbanchal University

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Ruby Maharjan