Sai Lakshmi Prasanna Seshabhattaru
Los Angeles,CA
Summary
Master’s graduate in Biotechnology and Bioinformatics with hands-on experience in immunology, molecular biology, and cell culture techniques. Skilled in DNA/RNA extraction, PCR/qPCR, Western blotting, ELISA, flow cytometry, and immunocytochemistry. Conducted a genetic study on FOXP3 polymorphism which is a master regulator of T cells, analyzing their role in immune regulation, which provides a strong foundation for T cell immunology research. Experienced in mammalian cell culture (primary and immortalized lines) and in vitro modeling. IACUC-certified in mouse handling with practical skills in genotyping, tagging, euthanasia, and organ, blood, and urine collection. Adept at maintaining accurate experimental documentation, adhering to GMP/GDP standards, and collaborating with interdisciplinary teams to ensure high-quality research outcomes. Motivated to expand expertise in small animal models and contribute to research on T cell function in acute kidney injury.
Overview
4
4
years of professional experience
Work History
CIRM Bridges Intern
Children’s Hospital Los Angeles
09.2024 - 08.2025
- Studied protective effects of the Fasting Mimicking Diet on podocyte function in Alport Syndrome using Western blotting and Glomerulus-on-a-Chip.
- Applied immunocytochemistry, ELISA, and flow cytometry to characterize kidney cell populations and immune markers.
- Performed mouse genotyping, tagging, euthanasia, and organ/blood/urine collection in compliance with IACUC standards.
- Cultured and maintained glomerular endothelial cells, tubular cells, and primary podocytes isolated via AutoMACS and FACS.
- Maintained precise experimental records, ensuring reproducibility and regulatory compliance.
API and Chemicals Sales and Logistics Assistant Coordinator
MM Kemicals
06.2022 - 07.2023
- Managed client acquisition, contract negotiations, and maintained strong customer relationships to drive sales growth.
- Oversaw compliant, timely shipment of products while streamlining sales and logistics operations for efficiency.Worked with internal teams and external stakeholders to align sales strategies with logistics capabilities.
Scientist I
VIMTA Labs
07.2021 - 03.2023
- Performed Sucralfate and Bleomycin assays to determine drug efficacy and analyzed free iron concentration in Iron Sucrose injections for leading pharmaceutical companies.
- Utilized UV Spectroscopy, Atomic Absorption Spectroscopy (AAS), and Capillary Electrophoresis for precise analytical testing and validation.
- Ensured compliance with Good Manufacturing Practices (GMP), Good Documentation Practices (GDP), Out-of Specification (OOS), and Corrective and Preventive Actions (CAPA) to maintain high-quality standards.
- Collaborated with project management teams to align testing procedures with client requirements and regulatory expectations.
- Prepared critical data for FDA audits, ensuring full compliance with industry regulations and Quality Assurance protocols.
- Maintained exceptional documentation practices, supporting accuracy, traceability, and regulatory readiness.
Education
MS. - Biotechnology and Bioinformatics
California State University Channel Islands
08.2025
Bachelor of Technology - Genetic Engineering
Bharath Institute of Higher Education and Research
05.2021
Skills
- T cell immunology, ELISA, Western blotting, ARMS-PCR, PCR, DNA/RNA extraction, immunocytochemistry, protein quantification/extraction
- Flow cytometry (FACS, BD FACSDiva) and analysis of immune cell populations
- Organ-on-a-chip experiments for kidney models
- Mouse genotyping, tagging, euthanasia
- Organ, blood, and urine collection for downstream analysis
- Basic tissue processing (paraffinizing, deparaffinizing, digestion)
- Primary cell isolation and culture (podocytes, glomerular endothelial cells, tubular cells)
- Mammalian cell culture (CHO-K, HEK293T, stem cells, progenitor cells)
- hiPSC colony identification/picking, NPC derivation, fibroblast isolation/reprogramming via electroporation
- Cell characterization (colony-forming unit assays, alkaline phosphatase staining, immunocytochemistry for markers such as Oct-4, GCTM-2)
Immunology & Molecular Biology
Animal Handling (IACUC-Certified)
Cell Culture
- UV Spectroscopy, Atomic Absorption Spectroscopy (AAS), Capillary Electrophoresis
- Zeiss & Leica fluorescence/confocal microscopy
- AutoMACS cell separation
- Protein docking and molecular modeling using bioinformatics tools
- GMP, GDP, SOP writing, laboratory documentation, FDA audit preparation
- Out-of-Specification (OOS) investigations, Corrective and Preventive Actions (CAPA)
- Regulatory submissions and quality assurance
- Microsoft Word, Excel, PowerPoint
- GraphPad Prism, LIMS, Image J, ANOVA statistical analysis
Techniques & Equipment
Compliance & Quality Systems
Data Analysis & Software
Timeline
CIRM Bridges Intern
Children’s Hospital Los Angeles
09.2024 - 08.2025
API and Chemicals Sales and Logistics Assistant Coordinator
MM Kemicals
06.2022 - 07.2023
Scientist I
VIMTA Labs
07.2021 - 03.2023
Bachelor of Technology - Genetic Engineering
Bharath Institute of Higher Education and Research
MS. - Biotechnology and Bioinformatics
California State University Channel Islands
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