Sara Welch
Bethel,OH
Summary
Versatile Molecular technologist bringing advanced understanding of clinical laboratory developed over 28 year career in field. Excellent critical thinking, complex problem-solving and decision-making abilities with good grasp of business requirements and industry trends. Ready to apply knowledge and abilities to a public service position.
Overview
37
37
years of professional experience
Work History
Tech III- Genetics
Genetics and Genomics Diagnostic Laboratory
Cincinnati
09.2011 - Current
- Clinical laboratory performing molecular diagnostic testing
- Tests performed include STR (bone marrow engraftment), BCR-ABL Quantitative analysis, real-time PCR allelic discrimination, Fragile X, Sanger Sequencing, sample extraction, and Next Generation panel analysis.
Pathology department at CCHMC
Cincinnati
06.2020 - 09.2020
- Helped with Simplexa COVID-19 testing for patients and employees
- PCR using DiaSorin Direct assay kits and equipment.
Research Assistant
Dr. Nancy Ratner
Cincinnati
12.2005 - 09.2011
- Research on project A of the NF1 Center Consortium
- Study of TC21 Rras 2 pathways and phenotype in Neurofibromatosis type 1 mouse models
- Project required extensive mouse characterization, tissue culture, biochemical analysis, and adenoviral stock preparation
- Generation of Schwann cells and neural spheres from E12.2 mice
- Updates and renewal of IBC protocols.
Research Assistant
Dr. Sandra Degen
Cincinnati
06.2003 - 12.2005
- Research project in the study of Ron receptor and one of the ligands HGFL
- Project required work with mouse model characterization and tissue culture
- Duties also included radiation safety.
Research Assistant
Dr. Mark Sussman
Cincinnati
09.1995 - 06.2003
- Research projects characterizing Tropomodulin in heart disease in overexpressing transgenic mouse models
- Generation of transgenic mouse models, characterization and analysis of hearts using tissue culture, rat cardiomyocyte isolation and adenoviral infection for in vitro experiments
- Responsible for lab set up, ordering and all radiation and biosafety protocols.
Lab and Facility Coordinator
Institute for Genetic Medicine
University of Southern California
06.1990 - 08.1995
- Coordinator for newly formed Institute for Genetic Medicine
- Attended architect and layout planning meetings for the laboratories
- Input into equipment and laboratory supply and storage of consumables
- Worked with Dr
- Mark Sussman on the initial Tropomodulin protein isolation in a fusion protein system
- Western, Southern blotting, HPLC and other methods used to purify an antibody to the Tropomodulin protein.
Research Assistant
Dr. Chia Y. Lee
Kansas University Medical Center
01.1987 - 05.1990
- Characterization of 2 staphylococcal bacteriophages L54a and phi 11 in Staphylococcus aureus
- Sanger Sequencing, microbiology, and biochemical techniques.
Education
Bachelor of Science in Microbiology -
South Dakota State University
Brookings, SD05.1986
Publications
- In vivo regulation of TGF-beta by R-Ras2 revealed through loss of the RasGAP protein NF1. Cancer Res 2012 Oct15;72(20): 5317-27. Patmore DM, Welch S, Fulkerson PC, Wu J, Choi K, Eaves D, Kordich JJ, Collins MH, Cripe TP, Ratner N.
- Nuclear targeting of Akt enhances kinase activity and survival of cardiomyocytes. Circ. Res. 2004 Apr 16;94(7): 856-9. Shiraishi I, Melendez, J, Ahn Y, Skavdahl M, Mruphy E, Welch S, Schafer E, Walsh K, Rosenzweig A Torella D, Nurzynska D, Kajstura J, Leri, A, Anversa P, Sussman MA.
- Activation of pyk2/related focal adhesion tyrosine kinase and focal adhesion kinase in cardiac remodeling. J Biol Chem. 2002 Nov 22;277(47): 45203-10. Melendez J, Welch S, Schaefer E, Moravec CS, Avraham S, Sussman MA.
- Cardiac-specific IGF-1 expression attenuates dilated cardiomyopathy in tropomodulin overexpressing transgenic mice. Circ Res 2002 apr 5; 90(6):641-6. Welch S, Plank D, Witt S, Glascock B, Schaefer E, Chimenti S, Andreoli AM, Limana F, Leri,A, Kajstura J, Anversa P, Sussman MA.
- Myocardial Akt activation and gender: increased nuclear activity in females versus males. Circ Res 2001 May 25;88(10):975-7. Camper-Kirby D, Welch S, Walker A, Shirasishi I, Setchell KD, Schaefer J, Anversa P, Sussman MA.
- Altered focal adhesion regulation correlates with cardiomyopathy in mice expressing constitutively active Rac 1. J Clin Invest. 2000 apr;105(7);875-86. Sussman MA, Welch S, Walker A, Klevitsky R, Hewett TE, Price RL, Schaefer E, Yager K.
- Hypertrophic defect unmasked by calcineurin expression in asymptomatic tropomodulin overexpressing transgenic mice. Cardiovasc Res. 2000 Apr;46(1);17-9. Sussman MA, Welch S, Walker A, Klevitsky R, Hewett TE, Will SA, Kimball TR, Price R, Lim HW, Molkentin JD.
- Pathogenesis of dilated cardiomyopathy: molecular, structural, and population analyses in tropomodulin-overexpressing transgenic mice. Am J Pthol. 1999 Dec; 155(6); 2101-13. Sussman MA, Welch S, Gude N, Khoury PR, Daniels SR, Krikpatrick D, Walsh RA, Price RL, Lim HW, Molkentin JD.
- Myofibril degeneration caused by tropomodulin overexpression leads to dilated cardiomyopathy in juvenile mice. J Clin Invest. 1998 Jan1;(1);51-61. Sussman MA, Welch S, Cambon N, Klevitsky R, Hewett TE, Price R, Witt SA, Kimball TR.
- Involvement of phosphorylation in doxorubicin-mediated myfibril degeneration. An immunofluorescence microscopy analysis. Circ Res 1997 Jan; 80(1) 52-61. Sussman MA, Hamm-Alvaraz SF, Vilalta PM, Welch S, Kedes L.
- Chicken skeletal muscle tropomodulin; novel localization and characterization. Cell Tissue Res. 1996 Aug; 285(2); 287-96. Sussman MA, Ito M, Daniels MP, Flucher B, Buranen S, Kedes L.
- Construction of single-copy integration vectors for Staphylococcus aureus. Gene 1991 July 15;103(1) 101-5. Lee CY, Buranen SL, Ye ZH.
- Sequence analysis and comparison of int and xis genes from staphylococcal bacteriophages L54a and phi 11. J Bacteriol. 1990 May; 172(5); 2568-75. Ye ZH, Buranen SL, Lee CY.
- Extent of the DNA sequence required in integration of staphylococcal bacteriophage L54a. J Bacteriol. 1989 Mar;17 (3); 1652-7. Lee CY, Buranen SL.
Timeline
Pathology department at CCHMC
06.2020 - 09.2020
Tech III- Genetics
Genetics and Genomics Diagnostic Laboratory
09.2011 - Current
Research Assistant
Dr. Nancy Ratner
12.2005 - 09.2011
Research Assistant
Dr. Sandra Degen
06.2003 - 12.2005
Research Assistant
Dr. Mark Sussman
09.1995 - 06.2003
Lab and Facility Coordinator
Institute for Genetic Medicine
06.1990 - 08.1995
Research Assistant
Dr. Chia Y. Lee
01.1987 - 05.1990
Bachelor of Science in Microbiology -
South Dakota State University
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