Sasha Chang
Portland,OR
Summary
A recent Reed College biology graduate with wet lab and animal husbandry knowledge and experience. Detail-oriented and eager to become a helpful team member. Takes initiative and enjoys problem-solving.
Overview
4
4
years of professional experience
Work History
Developmental Biology Researcher (PT)
Kara Cerveny
01.2023 - 05.2023
- Created a written protocol for the project below.
- Created a step-by-step video of the transplant surgery.
Developmental Biology Researcher (FT)
Kara Cerveny
05.2022 - 09.2022
- Investigated vasculogenesis and retinogenesis in the developing zebrafish eye.
- Conducted microinjections of mRNA and microsurgeries to transplant zebrafish embryo optic vesicles into the tail of developing embryos.
- Used confocal microscopy and light-sheet microscopy to visualize and analyze spatial and temporal expression of kdrl.
- Used software such as Imaris 3D imaging software and Nikon Microscopy software (NIS-Elements).
Research Lab Manager (PT)
Kara Cerveny
02.2021 - 08.2022
- Kept laboratory and zebrafish colony in compliance with applicable guidelines and laws.
- Trained staff and assigned tasks weekly.
- Conducted regular maintenance of Aquaneering™ filtration system which keeps ideal water parameters for the zebrafish.
- Genotyped (PCR- and HRMA-analyzed), transgenetically screened, and sexed fish stocks to maintain appropriate fish stock populations.
Developmental Biology Researcher (FT)
Kara Cerveny
06.2021 - 08.2021
- Investigated how retinoic acid activates tshz1 and nr6a1b in the developing zebrafish retina
- Created a transgenic line of fish expressing retinoic acid response elements.
- Conducted in situ hybridizations, microinjections, drug-treatments, and confocal microscopy.
- Used software such as Imaris 3D imaging software and Nikon Microscopy software (NIS-Elements).
Animal (Xenopus) Care Technician (PT)
Erik Zornik
01.2021 - 08.2021
- Performed daily tasks such as feeding xenopus (African clawed frogs) and logging water parameters.
- Performed regular maintenance of Aquaneering™ filtration system.
- Updated care practices for the colony as needed to maintain a higher standard of care.
Animal (Zebrafish) Care Technician (PT)
Kara Cerveny
01.2019 - 02.2021
- Performed daily maintenance and feeding of zebrafish colony (~2,000 fish).
- Prepared and maintained live food colonies (brine shrimp and rotifers).
- Cleaned and organized fish colony equipment and tanks.
- Recorded colony and water parameters.
Vet Parasitology Laboratory Technician (FT/PT)
IDEXX Laboratories
06.2020 - 12.2020
- Full-time July 2020 - September 2020 then part-time September 2020 - December 2020. Graveyard shift.
- Processed patient (animal) stool samples through fecal flotation and mounted them on microscope slides.
- Prepped patient stool samples prior to processing them through the Hamilton ELISA machine.
Animal (Xenopus) Care Technician
Erik Zornik
01.2019 - 03.2020
Developmental Biology Researcher (FT)
Kara Cerveny
05.2019 - 08.2019
- Investigated how the fibroblast growth factor pathway controls the pattern of neurogenesis in the zebrafish retina.
- Conducted fish pairings, drug-treatments, immunohistochemistry, genotyping, and confocal microscopy.
- Used software such as Imaris 3D imaging software, Nikon Microscopy software (NIS-Elements), R-studio, and ImageJ.
Education
Bachelor of Arts - Biology
Reed College
Portland, OR05.2023
Skills
- Laboratory skills and practices
- Animal husbandry
- Microscopy
- Microsurgery
- Problem-solving
- Animal handling
- Creativity
- Management
- Adaptability
- Decision-making skills
Timeline
Developmental Biology Researcher (PT)
Kara Cerveny
01.2023 - 05.2023
Developmental Biology Researcher (FT)
Kara Cerveny
05.2022 - 09.2022
Developmental Biology Researcher (FT)
Kara Cerveny
06.2021 - 08.2021
Research Lab Manager (PT)
Kara Cerveny
02.2021 - 08.2022
Animal (Xenopus) Care Technician (PT)
Erik Zornik
01.2021 - 08.2021
Vet Parasitology Laboratory Technician (FT/PT)
IDEXX Laboratories
06.2020 - 12.2020
Developmental Biology Researcher (FT)
Kara Cerveny
05.2019 - 08.2019
Animal (Zebrafish) Care Technician (PT)
Kara Cerveny
01.2019 - 02.2021
Animal (Xenopus) Care Technician
Erik Zornik
01.2019 - 03.2020
Bachelor of Arts - Biology
Reed College
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